Fig. 3

The manner in which EZH2, JMJD3 interacts with TET1 in spermatogonia. (A) The expression of EZH2 after EZH2 SiRNA in spermatogonia was detected by qRT-PCR. (B) qRT-PCR was used to detect the expression of EZH2 after co-transfection of EZH2 SiRNA and TET1 overexpression vector in spermatogonia. (C) The expression of JMJD3 in spermatogonia after JMJD3 SiRNA was detected by qRT-PCR. (D) qRT-PCR was used to detect the expression of JMJD3 after the co-transfection of JMJD3 SiRNA and TET1 overexpression vector in spermatogonia. (E) Bead plot of methylation sequencing results and methylation ratio after PCR amplification with primers designed for EZH2 and JMJD3(CpG-enriched region within the first 2000 bp of the promoter region), Filled circles represent methylated, empty circles represent unmethylated. (F) The JMJD3-TET1 protein interaction was detected by Co-IP technology. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig S3