Fig. 6

One-dimensional scatter plots of three selected miRNA expression (ΔCt) in mock and virus inoculated leaves of wheat cultivars Arapahoe and Mace at 18 °C and 27 °C. A lower ΔCt value represents higher expression of a) miRNA 397-5p, b) miRNA 398, and c) miRNA 9670-3p. Abundance from normalized counts is provided below each graph to provide comparison between RNAseq data and miRNAs expression levels obtained in RT-qPCR. Each dot represents the ΔCt value of a biological replicate. P-values for statistically significant samples are depicted above each sample. T: TriMV, W: WSMV, T + W: both viruses