Fig. 2

Evaluation and annotation of the newly assembled AealbF3 genome. a Protein sequences from AealbF3 was aligned with those of Ae. aegypti (Aeaeg), An. gambiae (Angam), Cu. quinquefasciatus (Cuqui), and Dr. melanogaster (Drmel) to assess the genic location of AealbF3. Syntenic blocks are linked between genomes in a circos plot. The chromosomal labels were shown around the circos. Brackets indicate the genus of each genomes. b Comparison of tandem element to confirm the genomic assembly. Similar results were observed among AealbF1, AealbF2, and AealbF3. LINE, long terminal repeat retrotransposon; SINE, short interspersed nuclear element; DNA, DNA transposon; LTR, long terminal repeat. “Raw” refers to the draft assembly; “Primary” indicates the removal of contigs lacking single-copy genes from the BUSCO dataset. c Evaluation of genome completeness using BUSCO. d Comparison of protein-coding genes and transcripts between AealbF3 and those of AealbF1 and AealbF2. e Comparison of CDS length, exon, and intron length among AealbF1, AealbF2, and AealbF3