Fig. 4

PGANT3 was associated with O-glycan biosynthesis. a Principal component analysis was conducted to compare the expression levels between ovaries and carcasses. b Volcano plots showed the differential expression between carcasses and ovaries. Five critical PSGs were labeled and the horizontal dashed line showed a p-value threshold of 0.05. “NB” denotes non-blood-fed mosquitoes, while “B” denotes blood-fed mosquitoes. c Venn plot indicated the common PSGs that were unregulated in the carcasses. Only genes with fold change > 2 were showed and colored in blue. d Tissue-specific expression of five PSGs using real-time PCR. Relative abundance of gene was compared with the reference gene RpS7. e Heatmap plot showed the RNAi results of differential expression genes. Total RNAs of mosquitoes after dsRNA-PGANT3 injection were performed RNA-sequencing, while wild-type ones were used as control. f KEGG analysis of down-regulated genes after RNAi. Pathways involved in O-glycan biosynthesis were colored in blue