Fig. 5

Artifact variant reduction in paired-sequencing samples. Changes of the somatic variants detected in samples prepared using the Rapid MaxDNA Lib Prep kit (sonication fragmentation) and 5 × WGS fragmentation mix kit (enzymatic fragmentation) before and after blacklist filtration. A Number of somatic variants in each of the 54 samples prepared using a Rapid MaxDNA Lib Prep kit before and after filtration with the constructed PBIVS (B). Average number and range of somatic variants in the 54 samples prepared using a Rapid MaxDNA Lib Prep kit, before and after filtration with the constructed PBIVS. C Number of somatic variants in each of the 54 samples prepared using a 5 × WGS fragmentation mix kit before and after filtration with PBPS. D Average number and range of somatic variants in the 54 samples prepared using a 5 × WGS fragmentation mix kit, before and after filtration with the constructed PBPS. E and F Comparison of somatic variants detected in samples prepared with a Rapid MaxDNA Lib Prep kit and 5 × WGS fragmentation mix kit after filtration with PBIVS and PBPS, respectively. G Venn diagram of somatic variants detected in the sonication fragmentation and enzymatic fragmentation samples