Fig. 6

Expression of ABF genes. A Phylogenetic analysis of the C. pepo and Arabidopsis ABF proteins. The yellow squares represent squash proteins, and the green circles represent Arabidopsis proteins used for comparison. The phylogenetic tree was built with Mega X using the Maximum Likelihood method and 1000 bootstrap replications. B Expression profiles of ABF genes in leaf and seed under different treatments and stages of germination. Data were normalized using log₂ FPKM and TBtools was used to draw the expression heatmap. The expression values assigned to a color gradient from low log₂ FPKM (green) to high log₂ FPKM (red) are shown on the right of figure. White asterisks indicate genes with significant differences in gene expression compared to a reference sample (red arrow) used as control (adjusted p-values < 0.05). For the leaf experiment, the reference control sample was the leaf of the untreated seedling growing under control conditions. For the seed experiment, the reference sample was the dry seed