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Fig. 2 | BMC Genomics

Fig. 2

From: Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Fig. 2

RA inhibits the proliferation of sheep primary myoblasts through BHLHE40. (A) Representative images of wound healing scratch assay after inhibition of BHLHE40. (B) Representative images of EdU staining assay after inhibition of BHLHE40. (C) Protein abundance of CDK4, Cyclin D1 and PCNA after inhibition of BHLHE40. (D) Representative images of wound healing scratch assay after overexpression of BHLHE40. (E) Representative images of EdU staining assay after overexpression of BHLHE40. (F) Protein abundance of CDK4, Cyclin D1 and PCNA after overexpression of BHLHE40. (Mean ± SEM; n = 3 in each group, *P < 0.05. Values with different letters indicated significant differences. The blots were cut prior to hybridisation with antibodies, and images of blots for all replicates were provided in the additional file 4)

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BMC Genomics

ISSN: 1471-2164

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