Fig. 3

RA promotes the differentiation of sheep primary myoblasts through BHLHE40. (A) Representative images of MHC immunofluorescence staining after inhibition of BHLHE40. (B) Fusion index. (C) Relative mRNA contents of MYF5, MYOD and MYOG after inhibition of BHLHE40. (D) Protein abundance of MyoD, MyoG and MHC after inhibition of BHLHE40. (E) Representative images of MHC immunofluorescence staining after overexpression of BHLHE40. (F) Fusion index. (G) Relative mRNA contents of MYF5, MYOD and MYOG after overexpression of BHLHE40. (H) Protein abundance of MyoD, MyoG and MHC after overexpression of BHLHE40. (Mean ± SEM; n = 3 in each group, *P < 0.05, **P < 0.01. Values with different letters indicated significant differences. The blots were cut prior to hybridisation with antibodies, and images of blots for all replicates were provided in the additional file 4)