Fig. 4

BHLHE40 targets and inhibits ID3 gene expression. (A) Relative mRNA content of ID3 in control and Vitamin A groups lamb. (B) Relative mRNA content of ID3 during differentiation of myoblasts. (C) Relative mRNA content of ID3 in control and RA groups of differentiated myoblasts. (D) Protein abundance of ID3 in control and RA groups of differentiated myoblasts. (E) Relative mRNA content of ID3 after inhibition or overexpression of BHLHE40. (F) Protein abundance of ID3 after inhibition of BHLHE40. (G) Protein abundance of ID3 after overexpression of BHLHE40. (H) Dual luciferase reporter assay. (Mean ± SEM; n = 3 in each group, *P < 0.05, **P < 0.01. The blots were cut prior to hybridisation with antibodies, and images of blots for all replicates were provided in the additional file 4)