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Fig. 5 | BMC Genomics

Fig. 5

From: Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Fig. 5

ID3 promotes the proliferation of sheep primary myoblasts. (A) Representative images of wound healing scratch assay after inhibition of ID3. (B) Representative images of EdU staining assay after inhibition of ID3. (C) Protein abundance of CDK4, Cyclin D1 and PCNA after inhibition of ID3. (D) Representative images of wound healing scratch assay after overexpression of ID3. (E) Representative images of EdU staining assay after overexpression of ID3. (F) Protein abundance of CDK4, Cyclin D1 and PCNA after overexpression of ID3. (Mean ± SEM; n = 3 in each group, **P < 0.01. The blots were cut prior to hybridisation with antibodies, and images of blots for all replicates were provided in the additional file 4)

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BMC Genomics

ISSN: 1471-2164

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