Fig. 6

ID3 inhibits the differentiation of sheep primary myoblasts. (A) Representative images of MHC immunofluorescence staining after inhibition of ID3. (B) Fusion index. (C) Relative mRNA contents of MYF5, MYOD and MYOG after inhibition of ID3. (D) Protein abundance of MyoD, MyoG and MHC after inhibition of ID3. (E) Representative images of MHC immunofluorescence staining after overexpression of ID3. (F) Fusion index. (G) Relative mRNA contents of MYF5, MYOD and MYOG after overexpression of ID3. (H) Protein abundance of MyoD, MyoG and MHC after overexpression of ID3. (Mean ± SEM; n = 3 in each group, *P < 0.05, **P < 0.01. The blots were cut prior to hybridisation with antibodies, and images of blots for all replicates were provided in the additional file 4)