Fig. 10

Analysis of upstream regulatory factors of PheSnRK2.9 and verification of dual luciferase. a Yeast one-hybrid assay. Positive control is pGADT7 53 + pHIS2 53 and negative control is pHIS2 53 + pGADT7 Rec2. pro-PheSnRK2.9 represent the PheSnRK2.9 gene promoter region; b Schematic diagram of the dual luciferase vector construction. c The analysis results of relative fluorescence activity. The luminescence ratio of firefly LUC to Renilla LUC was determined according to a dual luciferase reporter system. Experiments were repeated at least three times and results are expressed as mean ± standard deviation. The differences in dual luciferase enzyme activity between each transcription factor binding to the promoter and the control reached significance, with the following respective p-values: 0.00104 (PheNAC9), 0.00006 (PheNAC10), 0.00056 (PheNAC11), 0.00273 (PheIAA1.2), 0.00919 (PheIAA4.2).