Fig. 7

Overexpression of PheSnRK2.9 inhibited root growth and leaf starch degradation in Arabidopsis thaliana. a overexpression PheSnRK2.9 the Arabidopsis root phenotype. b Corresponding Arabidopsis root length statistics in A. Significance was assessed using Student’s t-test, one-way analysis of variance, and multiple comparisons. Column height represents the average root length (± SE, n = 3), where p < 0.05 indicates significant difference. Different lowercase letters indicate a significant difference. c Protein sequence comparison between AtSnRK2.4 and PheSnRK2.9. DNAMAN software was used to compare the protein sequences, and the underline indicated that the candidate residues binding PA in Arabidopsis thaliana were also present in Moso bamboo. d 6-week-old Arabidopsis thaliana plant phenotype overexpressing PheSnRK2.9. e 6-week Arabidopsis thaliana rosette leaves. fArabidopsis thaliana leaf starch staining after 24 h dark treatment. Col-0: Columbia wild type; 35 S::eGFP: Transgenic line containing eGFP label; 35 S::PheSnRK2.9-1, 35 S::PheSnRK2.9-2, 35 S::PheSnRK2.9-3,: Different transgenic lines. Bars = 1 cm