Fig. 8

Seed germination and subcellular localization under salt stress. a Overexpressed PheSnRK2.9 Arabidopsis seed germination on a medium containing 100 mM NaCl. b Seed germination rate statistics. We evaluated the significance of seed germination of different transgenic lines on each day using Student’s t-test, one-way ANOVA, and multiple comparisons. (n = 35, ± SE), p < 0.05. The germination rate of the seeds on the first and second days was 0 for both, without considering inter-group differences. Starting from the third day, the inter-group differences in germination rate of transgenic seeds were found to be significant, with the following respective p-values: 0.00059 (3d), 0.00074 (4d), 0.05656 (5d), 0.51261 (6d), 0.60815 (7d). c Subcellular localization of PheSnRK2.9 in leaves of Nicotiana benthamiana. WT: Col-0; OE2.9-1/OE2.9-2/OE2.9-3: different transgenic lines. Bar = 100 μm