Fig. 7

Effect of TFAP4 overexpression on differentiation of bovine satellite cells. Cells were transfected with a TFAP4 expression plasmid (oeTFAP4) or a negative control plasmid (CTRL) and induced to differentiate into myofibers for 4 days. A Representative images of cells on days 0, 1, 3 and 4 of differentiation. B mRNA expression levels of myofiber marker genes MYH2, MYH3, MYOG, CKM, and MYMK as well as TFAP4. *P < 0.05, ***P < 0.001 (n = 6). P = 0.070 and 0.078 for MYH2 and MYOG, respectively. C Western blot analyses of TFAP4, MHC, CKM, and MYOG proteins. β-Tubulin was detected as a loading control. Shown are cropped representative western blots. Full-length western blots are presented in Additional file 13. D Quantitative analysis of western blots. *P < 0.05 (n = 3) for TFAP4, CKM, and MYOG; P = 0.089 for CKM