Table 1 Experimental conditions for each species, including the blood volume, the use of the additional DNase I treatment, total RNA input, the time of depletion and the number of PCR cycles
From: A single workflow for multi-species blood transcriptomics
Species | Blood Volume (mL) | Two DNase I treatments | Total RNA input (ng) | Time depletion (h) | Number of PCR cycles |
|---|---|---|---|---|---|
Human | 2.5 | Yes | 250 | 4.5 | 15 |
NHP | 0.25 | Yes | 100 | 4.5 | 16 |
Rabbit | 1 | Yes | 250 | 4.5 | 15 |
Mouse | 0.15 | No | 50 | 4.5 | 16 |