Fig. 4

Effects of larval Leguminivora glycinivorella exposures to transgenic Glycine max cultivar GP03-8–23 expressing the Bacillus thuringiensis (Bt) Cry1Ac pesticidal protein compared to a non-Bt cultivar. A Principal component analysis (PCA) plot of mean log₂CPM estimates among transcripts for genes along principal coordinates 1 (PC1) and 2 (PC2) showing two clusters corresponding to treatment groups. Variation between treatments precited B 204 genes with significant differential expression (DE) and C 10 genes showing significant differential alternative splicing (DAS). Similarly, at the transcript level D PCA demonstrate distinct clustering of read abundances among replicates of each treatment along PC1 and PC2. Intra-treatment comparisons predicted E DE of 39 transcripts and F 12 with predicted differential transcript usage (DTU)