Fig. 3
From: HOXA9 gene inhibits proliferation and differentiation and promotes apoptosis of bovine preadipocytes
HOXA9 inhibits the proliferation of bovine adipocytes. (A.D) Relative expression level of mRNA of proliferation markers after overexpression/interference of HOXA9. (B.E) Relative expression level of protein of proliferation markers after overexpression/interference of HOXA9. (C.F) The viability of cells was detected by CCK8 after overexpression/interference of HOXA9. (G.H) Cell proliferation rate was detected by EdU (scale bar = 500 μm) after overexpression/interference of HOXA9. (I.J) The cell cycle analysis after overexpression/interference of HOXA9. GAPDH as a standardized reference gene. The data were expressed as mean ± SEM, n = 3, * P < 0.05, ** P < 0.01