Table 2 Primer information
From: HOXA9 gene inhibits proliferation and differentiation and promotes apoptosis of bovine preadipocytes
Gene | Primer sequences (5’-3’) | Products size/bp | Annealing temperature/℃ |
|---|---|---|---|
HOXA9 | F:CCACGCTTGACACTCACACTT R:GCCGCTCTCATTCTCAGGATTG | 122 | 60 |
GAPDH | F:CCAACGTGTCTGTTGTGGAT R:CTGCTTCACCACCTTCTTGA | 80 | 60 |
PPARγ | F:AGGATGGGGTCCTCATATCC R:GTCAGCTCTTGGGAACGGAA | 137 | 60 |
CEBPα | F:TGGACAAGAACAGCAACGAG R:TTGTCACTGGTCAGCTCCAG | 130 | 60 |
CEBPβ | F:TTCCTCTCCGACCTCTTCTC R:CCAGACTCACGTAGCCGTACT | 79 | 60 |
FABP4 | F: AAGTCAAGAGCATCGTAA R:CCAGCACCATCTTATCAT | 111 | 60 |
LPL | F:ACGATTATTGCTCAGCATGG R:ACTTTGTACAGGCACAACCG | 130 | 60 |
CDK1 | F:GAAGGGGTTCCTAGTACTGC R:ATGAACTGACCAGGAGGG | 176 | 60 |
CDK2 | F:ATGAACTGACCAGGAGGG R:GCCAGGAGTTACTTCTATGC | 115 | 60 |
PCNA | F:GAACCTCACCAGCATGTCCA R:TACTAGTGCCAACGTGTCCG | 97 | 60 |
CCNA2 | F:ACACAGTCACAGGACAAAGC R:TCTGAGGTAGGTCTGGTGAA | 107 | 60 |
CCNB1 | F:TGGAGAGGTTGATGTTGAGC R:TCTGAGAAGGAGGAAAGTGC | 95 | 60 |
CCND1 | F:TGGTCCTGGTGAACAAACTC R:ATCTGCTTGTTCTCCTCGGC | 106 | 60 |
CCNE2 | F:GCTTATGTCACTGATGGTGCTTG R:TTAGCCAGGAGATGACCGTTAC | 112 | 60 |
BAX | F:GAGATGAATTGGACAGTAACA R:TTGAAGTTGCCGTCAGAA | 118 | 60 |
BAD | F:TCCCAGAGTTTGAGCAGAGTGA R:TTAGCCAGTGCTTGCTGAGAC | 108 | 60 |
BCL2 | F:ATGTGTGTGGAGAGCGTCAA R:GAGACAGCCAGGAGAAATCA | 181 | 60 |