Fig. 3

Differentially abundant proteins of SCFA and CONT by TMT-based quantitative proteome analysis in YRECs(n = 3). (a) Differentially abundant proteins in two comparison groups were classified according to molecular functions (MF), cellular components (CC), and biological processes (BP) using the enriched GO terms. The graphic displays the enrichment outcomes in three categories with a maximum of 20 in each (P-value ≤ 0.05). (b) KEGG pathway enrichment of differentially abundant proteins. The hypergeometric test’s P-value is indicated by the dot color. The differential protein count in the corresponding pathway is denoted by the size of the dots. (c) Structural domain enrichment bubble diagram of differentially abundant proteins. (d) The evaluation of differentially abundant proteins’ subcellular location. Integrative multi-omic data analysis revealed metabolites and proteins that could be linked to high concentration of SCFA-induced injury in YRECs. (e) The bubble map shows the enrichment of DEMs and DEPs in the same KEGG pathways. The horizontal coordinate is the ratio of differential metabolites or proteins improved in the pathway to the number of metabolites or proteins annotated in the pathway (Ratio). The metabolome-proteome co-enriched to the KEGG pathway is represented along the vertical coordinate