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Fig. 4 | BMC Genomics

Fig. 4

From: Metabolomics and proteomics insights into subacute ruminal acidosis etiology and inhibition of proliferation of yak rumen epithelial cells in vitro

Fig. 4

Cytotoxic damage induced by high concentration of SCFA exposure to YRECs. (a) Effects of high concentration SCFA exposure YRECs on the mRNA expression levels of filamin, ITGA5, ARPC5, ACTR3, ACTN4, PCNA, CCNB1, NFKB2, TRAF2, CHUK, PTGS2, ATG3, and TNF-α for 24 h. (b) The morphology and fluorescence intensity of mitochondrial staining in SCFA exposure YRECs. (Magnification 100×; red: Mitochondria). (c) Detection of cell cycle process in cells by flow cytometry and proportion of cells in the G0/G1, G2/M, and S phases. (d) Detection of ROS levels in cells. The format of mean ± SEM (n = 3) was used to present the data. *, ** and *** demonstrate significant deviations from the control at P < 0.05, P < 0.01 and P < 0.001 respectively

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BMC Genomics

ISSN: 1471-2164

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