Fig. 5

Differentially abundant proteins of Aci and CONT by TMT-based quantitative proteome analysis in YRECs(n = 3). (a) Differentially abundant proteins in two comparison groups were classified according to molecular functions (MF), cellular components (CC), and biological processes (BP) using the enriched GO terms. The figure shows the enrichment results in three categories, up to 20 of each (P-value ≤ 0.05), and the percentage of the vertical coordinate represents x/n in the table. (b) Proteins with differential levels of abundance are enriched in KEGG pathways. The P-value from the hypergeometric test is depicted through the color variation of each dot in the graph. Each dot’s size corresponds to the number of differential proteins identified within the corresponding pathway. (c) Structural domain enrichment bubble diagram of differentially abundant proteins. (d) Subcellular localization analysis of differentially abundant proteins. Integrative multi-omic data analysis revealed metabolites and proteins that may be associated with low pH acidic environment-induced injury in YRECs. (e) The bubble map shows the enrichment of DEMs and DEPs in the same KEGG pathways. The ratio of differential metabolites or proteins to the total number of metabolites or proteins in the pathway is depicted on the horizontal axis (Ratio). On the vertical axis, the value reflects the extent of co-enrichment between the metabolome and proteome within the KEGG pathway