Table 3 Primer information
Gene Number | Primer Name | Sequences(5’to 3’) | Annealing Temperature TM |
|---|---|---|---|
1 | 18 S -F | ATTCTATGGGTGGTGGTGCAT | 60 |
18 S -R | TCAAACTTCGCGGCCTAAA | ||
2 | > LOC112175470-F | CCAGTCTTGTTCTTCTCTATGTC | 60 |
> LOC112175470-R | CATGTCATTCCCGTACTTCTC | ||
3 | > LOC112164757-F | AGACGAGGAGCAAGCATC | 60 |
> LOC112164757-R | CGAAACCAACACAACGAGAA | ||
4 | > LOC112182880-F | CTGGAGCCTAAACTTTCAAGATC | 60 |
> LOC112182880-R | CCCATCACTCATCAAACCTTAC | ||
5 | > LOC112189201-F | TGAACCACATGATCTACGA | 60 |
> LOC112189201-R | CTTCACCCAGAATCAGTATG | ||
6 | > LOC112182926-F | CCATCATCTTCAACAAGGACAG | 60 |
> LOC112182926-R | GATTTGAAAGGAATGGCTCTGA |