Fig. 3

Disruption of miR-542-3p enhances the proliferation of myoblast. A The expression of miR-542-3p in C2C12 myoblasts at 48 h after its inhibitor treatment was assayed by RT-qPCR. B The mRNA levels of CCND1, CCND2, CCNB1, CDK2, CDK4, and P53 in the miR-542-3p inhibitor and inhibitor NC groups. C, D The western blotting bands of proliferation markers in the miR-542-3p inhibitor and inhibitor NC groups (C) and their quantitative analysis (D) by image J software. The membrane was cleaved before hybridization with the antibody, and all protein bands (CCND1, CCNB1, and β-actin) in Fig. 3C were from the same samples. 4 biological replicates were detected, 3 of which were shown in the Fig. 3C and full-length blots were presented in Supplementary Fig. S3. E, F The images of EdU staining (E) and percentage of the EdU positive cells (F), scale bar: 100 μm. G Cell viability was measured by CCK-8. n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001