Fig. 6

miR-542-3p directly targets the Pten gene. A Prediction of target genes of miR-542-3p using online software, including DIANA, TargetScan 7.2, and miRDB. B The complementary pairing of miR-542-3p and the potential target gene Pten wild type 3’-UTR or its mutated 3’-UTR. C Diagram of the construction of dual-luciferase reporter vectors containing the Pten 3’-UTR sequences of wild type or mutant. hRluc-neo fusion means renilla luciferase; Luc2 means firefly luciferase. D 293 T cells were co-transfected with Pten-3’-UTR wild or mutant dual-luciferase vector and the miR-542-3p mimic or mimic NC. The relative luciferase activity was assayed at 48 h after transfection. E, F The mRNA level of Pten in the miR-542-3p mimic (mimic) and mimic NC (E), miR-542-3p inhibitor (inhibitor) and inhibitor NC (F) were determined by RT-qPCR. G, I, H, J Protein level of Pten was detected by western blotting after overexpression (G, H) and inhibition (I, J) of miR-542-3p. The membrane was cleaved before hybridization with the antibody, and all protein bands in Fig. 6G and protein bands in Fig. 6I were from the same sample, respectively. Full-length blots are presented in Supplementary Fig. S6. n = 3, * P < 0.05, ** P < 0.01