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Fig. 8 | BMC Genomics

Fig. 8

From: MicroRNA-542-3p targets Pten to inhibit the myoblasts proliferation but suppresses myogenic differentiation independent of targeted Pten

Fig. 8

miR-542-3p inhibits myoblasts differentiation. A The mRNA level of Pten, MYOG, MYOD1, MYHC, MYF5, and MYF6 in the miR-542-3p inhibitor, si-Pten, miR-542-3p inhibitor + si-Pten, and control groups were detected by RT-qPCR. B Anti-Myosin heavy chain (MYHC) immunofluorescence staining after the transfection of miR-542-3p inhibitor, si-Pten, miR-542-3p inhibitor + si-Pten, and control groups. DAPI (blue), cell nuclei, scale bar: 100 μm. C MYHC immunofluorescence intensity analysis by image J software. D Differentiation index. E Myotube fusion index. n = 3, * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

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