Table 2 Primer sequences for a reverse transcription-quantitative polymerase chain reaction
Gene | Forward | Reverse |
|---|---|---|
miR-542-3p | 5’-GCGCGTGTGACAGATTGATAA-3’ | 5’-AGTGCAGGGTCCGAGGTATT-3’ |
miR-542-3p Stem loop sequence | 5’-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTTCAG-3’ | |
CCNB1 | 5’-CTTGCAGTGAGTGACGTAGAC-3’ | 5’-CCAGTTGTCGGAGATAAGCATAG-3’ |
CDK2 | 5’-CAAAGCCAAGCACGTAGAGAC-3’ | 5’-TGCACCACATATTGACTGTCC-3’ |
CDK4 | 5’-CTGAACCGCTTTGGCAAGAC-3’ | 5’-GCCCTCTCTTATCGCCAGAT-3’ |
CCND1 | 5’-TAGGCCCTCAGCCTCACTC -3’ | 5’-CCACCCCTGGGATAAAGCAC-3’ |
CCND2 | 5’-GAGTGGGAACTGGTAGTGTTG-3’ | 5’-CGCACAGAGCGATGAAGGT-3’ |
P53 | 5’-CAACAAATGCTGGCTACTAAGGA-3’ | 5’-CACGAGTTTTCCGTTGCTCA-3’ |
MYOG | 5’-GAGACATCCCCCTATTTCTACCA-3’ | 5’-GCTCAGTCCGCTCATAGCC-3’ |
MYOD1 | 5’-CCACTCCGGGACATAGACTTG-3’ | 5’-AAAAGCGCAGGTCTGGTGAG-3’ |
MYHC | 5’-GCGAATCGAGGCTCAGAACAA-3’ | 5’-GTAGTTCCGCCTTCGGTCTTG-3’ |
MYF5 | 5’-AAGGCTCCTGTATCCCCTCAC-3’ | 5’-TGACCTTCTTCAGGCGTCTAC-3’ |
MYF6 | 5’-AGAGGGCTCTCCTTTGTATCC-3’ | 5’-CTGCTTTCCGACGATCTGTGG-3’ |
U6 | 5’-CTCGCTTCGGCAGCACA-3’ | 5’-AACGCTTCACGAATTTGCGT-3’ |
18S | 5’-CGCGGTTCTATTTTGTTGGT-3’ | 5’-AGTCGGCATCGTTTATGGTC-3’ |
RPL7 | 5’-ACGGTGGAGCCTTATGTGAC-3’ | 5’-TCCGTCAGAGGGACTGTCTT-3’ |
Pten | 5’-TGGATTCGACTTAGACTTGACCT-3’ | 5’-GCGGTGTCATAATGTCTCTCAG-3’ |