Fig. 5
From: Evolutionary genetics of pulmonary anatomical adaptations in deep-diving cetaceans
The convergent site of SFTPC gene enhances pulmonary fibrosis in marine mammals.SFTPC overexpression in A549 cell lines, Western bloy analyses of pulmonary fibrosis markers(A) and apoptotic marker(C). (B)CCK8 assays detected the effects of after overexpressing SFTPC on the proliferation of A549 cell lines. EV is Lev-mCherry-Puro- CMV-N3xFlag-control, M is Lev-mCherry-Puro-CMV-N3xFlag-Mouse-SFTPC, W is Lev- mCherry-Puro-CMV-N3xFlag-BI-SFTPC, M(mut) is Lentivirus-mCherry-Puro-CMV- N3xFlag-Mouse-mutSFTPC, W(mut) is Lentivirus-mCherry-Puro-CMV-N3xFlag-BI- mutSFTPC. Date repressen the mean ± S.D.*p < 0.05,**p < 0.01. The cell lysates were subjected to Western blot analyses using indicated antibodies with Tubulin as a loading control. The blots/gels displayed in the figure represent cropped versions, delineated by black dividing lines. Full-length blots/gels are presented in Fig S5