Fig. 2

Changes in and expression regulation by H3K27me3 under FLHS. A Principal component analysis (PCA) was performed on ChIP-seq data for liver tissues in FLHS (red) and control (blue) groups. First principal component (PC1) accounts for largest possible variance in dataset. Second principal component (PC2) was also calculated, under the condition that it is uncorrelated with (i.e., perpendicular to) PC1. B The proportion of genomic features of H3K27me3 peaks was compared with genomic features. Distal intergenic regions refer to genomic regions except downstream (≤300) and promoter regions. C Metaplot of normalized H3K27me3 signal over the gene body of protein-coding genes in control and FLHS hens spanning from –3 kb upstream of TSS to + 3 kb downstream of the TES. D Gene expression levels as a function of H3K27me3 modification in FLHS hens. P values were calculated by a Student’s t test. ***P < 0.001; **P < 0.01; *P < 0.05