Fig. 3

Centromeric methylation features of Chlorella sorokiniana NS4-2. and Chlorella pyrenoidosa DBH. A Methylation density distribution of C. sorokiniana NS4-2 chromosome 1. The red area represents the centromere. The close-up view shows, from top to bottom, the distribution of methylation density, CENH3 ChIP-seq signals, and the location of centromeric regions (parallel red band). Within the centromeric region, ChIP-seq peaks called by MACS2 are shown in light green and non-peaks in orange; (B) Box and violin plots showing methylation density in C. sorokiniana NS4-2 for centromeric (CEN) and non-centromeric (non-CEN) regions, and for peaks (CEN_peaks) and non-peaks (CEN_nonPeaks) within the former; (C) Box and violin plots showing methylation density in C. pyrenoidosa DBH for CEN and non-CEN regions. The statistical analysis was conducted via the Wilcoxon test