Fig. 5

N-MYC, PDPK1, and WDR5 mediated regulation of mitotic genes. (a) Western blot showing proteins co-immunoprecipitated following induction of wild-type (WT) FLAG-epitope tagged PDPK1 in indicated cells compared to that of FLAG-epitope tagged PDPK1 with a point mutation that prevents direct binding of WDR5 (R3A). DOX indicates samples in which PDPK1 expression is induced using doxycycline. When WDR5 cannot interact with PDPK1, N-MYC binding is reduced. A similar effect is also observed across all N-MYC amplified cell lines (n = 3 biological replicates per cell line). Uncropped blots are presented in Additional File 3: Figure S1. (b) Comparison of 403 genes that are commonly regulated by N-MYC and PDPK1 to genes regulated in either direction following WDR5 WIN-site inhibition in CHP-134 cells using the small molecule C6 [21]. (c) Gene enrichment analysis for the 55 genes in (b) that are commonly regulated by N-MYC, PDPK1, and WDR5 using ShinyGO, GO: Cellular Component. (d) Heatmap showing log2(fold change) for the 55 genes in (b) that are commonly regulated by N-MYC, PDPK1, and WDR5