Fig. 1

Deletion engineered at the murine Dfnb1 locus. A Map of the chromosome 14 region targeted in this study, including genes of interest. Scissors indicate the localization of CRISPR guides, and arrows show the primers used to test for the deletion (primer arrows not to scale). B Genomic sequences of Gjb6 exon 3 and Cryl1 exon 1 regions, showing the location of the CRISPR guides, deletion breakpoints and primers that were used. Exonic sequences appear in blue bold capital letters, primers are highlighted in green background and underlined, and CRISPR guides are marked with different background colors. The actual breakpoints in each region are indicated by a red zigzag line, as deduced from the Sanger sequence of A1-B2 breakpoint PCR (bottom)