Fig. 3

Cx30 expression in the cochlea of P30 Dfnb1^em274 HOM mice. Representative micrographs of cochlear paraffin sections stained with hematoxylin–eosin (A, B) or immunostained against Cx30 (C, D), showing the basal region of WT (A, C) and Dfnb1^em274 HOM (B, D) mice. WT mice present a normal cochlear cytoarchitecture and Cx30 expression in the spiral limbus, organ of Corti (surrounding supporting cells, arrows in C) and lateral wall. Dfnb1^em274 HOM mice show a complete absence of Cx30 expression (D) and gross alterations in the Reissner membrane, tectorial membrane, and organ of Corti, compared to WT (B). LW, lateral wall; RM, Reissner membrane; SG, spiral ganglion; SL, spiral limbus; SM, scala media; ST, scala tympani; sTV, stria vascularis; SV, scala vestibuli. Scale bar: 100 mm