Fig. 6
Gjb2 expression in the whole cochleae of wild-type (WT), heterozygous (HET), and homozygous (HOM) P30 Dfnb1em274 mice. Gjb2 expression was quantified by using real-time qPCR. The Y axis indicates the relative amounts of Gjb2 transcript with regard to the expression of the reference gene Hprt, as calculated according to the mathematical model of Pfaffl [34]. Points on the graph correspond to three different cDNA concentrations for one cochlea from each mouse (2 WT, 2 HET, 2 HOM), each point being the mean of two independent experiments. Horizontal lines show the mean of the expression level of Gjb2 for each genotype