Table 1 Mobile phase solvent composition and gradient protocol.
Time (min) | Percent Solvent A | Percent Solvent B | Percent Solvent C |
|---|---|---|---|
0.00 | 94.00 | 5.00 | 1.00 |
30.00 | 64.00 | 35.00 | 1.00 |
34.20 | 59.00 | 40.00 | 1.00 |
35.00 | 39.00 | 60.00 | 1.00 |
38.20 | 39.00 | 60.00 | 1.00 |
38.30 | 0.00 | 99.00 | 1.00 |
39.50 | 0.00 | 99.00 | 1.00 |
40.50 | 94.00 | 5.00 | 1.00 |
50.00 | 94.00 | 5.00 | 1.00 |