Figure 3

TCSTs regulate amylovoran biosynthesis and gene expression. (A) Amylovoran production of E. amylovora WT and TCST mutants in vitro. Bacterial strains were grown in MBMA media with 1% sorbitol for 48 hrs at 28°C with shaking. The amount of amylovoran was measured with the CPC assay and normalized to a cell density of 1. Amylovoran operon (amsA-L) deletion mutant (Δams) was used as a negative control [47]. (B) Gene expression of the amsG gene in WT and TCST mutants in vitro. GFP intensity in WT and TCST mutants containing amsG promoter-gfp fusion plasmid was measured by flow cytometry. GFP-A: Green fluorescence protein absorbance; Count: Number of cells.