From: Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing
Experiment # | Experiment ID | Barcode | Sample | DNA Concentration (ng/φL) | A260/280 | Total reads | Mapped reads | % Mapping | Threshold |
---|---|---|---|---|---|---|---|---|---|
1 | PolII_Rep1 | ACGT | PolII | 16.6 | 1.83 | 1110177 | 623328 | 56.15 | 14 |
2 | Input_CATT | CATT | Input | 51.6 | 1.85 | 2526689 | 1719855 | 68.07 | Not being scored |
3 | Cse4_Rep1 | GTAT | Cse4 | 12.0 | 1.99 | 2606497 | 1080537 | 41.46 | 81 |
4 | Ste12_Rep1 | TGCT | Ste12 | 11.6 | 1.85 | 2020432 | 1072428 | 53.08 | 42 |
5 | Input_ACGT | ACGT | Input | 66.9 | 1.80 | 4678579 | 2857615 | 61.08 | Not being scored |
6 | Cse4_Rep2 | CATT | Cse4 | 8.6 | 1.85 | 679315 | 357384 | 52.61 | 25 |
7 | Ste12_Rep2 | GTAT | Ste12 | 9.4 | 1.86 | 2316023 | 1067739 | 46.10 | 44 |
8 | PolII_Rep2 | TGCT | PolII | 15.5 | 2.00 | 3991833 | 2561147 | 64.16 | 12 |
9 | PolII_Rep3 | ACGT | PolII | 9.7 | 1.97 | 2661304 | 1725793 | 64.85 | 42 |
10 | Input_NB | None | Input | 60.4 | 1.83 | 2432221 | 1582226 | 65.05 | Not being scored |
11A* | Cse4_Rep3 | None | Cse4 | 10.7 | 1.69 | 1168583 | 239580 | 20.50 | 60 |
11B* | Cse4_Rep3 | None | Cse4 | 10.7 | 1.69 | 3245287 | 583876 | 17.99 | |
12A* | Ste12_Rep3 | None | Ste12 | 9.1 | 1.64 | 2106917 | 473965 | 22.50 | 39 |
12B* | Ste12_Rep3 | None | Ste12 | 9.1 | 1.64 | 2668067 | 551040 | 20.65 | |
13 | PolII_Rep4 | None | PolII | 18.8 | 1.94 | 4649077 | 3151068 | 67.78 | 40 |
14 | Input_NB | None | Input | 60.4 | 1.83 | 4373256 | 2455181 | 56.14 | Not being scored |
15 | Input_ACGT | ACGT | Input | 66.9 | 1.80 | 873037 | 529038 | 60.60 | 5 |
16 | Input_CATT | CATT | Input | 51.6 | 1.85 | 799430 | 479546 | 59.99 | 5 |
17 | Input_GTAT | GTAT | Input | 34.6 | 1.69 | 1380820 | 850262 | 61.58 | 6 |
18 | Input_TGCT | TGCT | Input | 68.1 | 1.78 | 611123 | 362458 | 59.31 | 4 |