Skip to main content

Table 1 Microarray expression data for iron induced genes

From: Extending the models for iron and sulfur oxidation in the extreme Acidophile Acidithiobacillus ferrooxidans

ID NC011761

Gene

Function

log2 ratio median

One sample

t-Test

(p-value)

Proteomic data: strain/references

pet I operon

AFE_3111

petC1

ubiquinol-cytochrome c reductase, cytochrome c 1 subunit

3,7

0,00*

 

AFE_3110

petB1

ubiquinol-cytochrome c reductase, cytochrome b subunit

4,4

0,00*

 

AFE_3109

petA1

ubiquinol-cytochrome c reductase, iron-sulfur subunit

3,7

0,00*

CCM 4252/[24]

AFE_3108

sdrA1

oxidoreductase, short-chain dehydrogenase/reductase family

3,9

0,00*

 

AFE_3107

cycA1

cytochrome c 4

3,7

0,00*

 

rus operon

AFE_3153

cyc2

cytochrome c

2,5

0,00*

ATCC 33020/[7]

AFE_3152

cyc1

cytochrome c 552

2,8

0,00*

ATCC 33020/[7]

CCM 4253/[24]

ATCC 19859/[25]

AFE_3151

cup

conserved hypothetical protein

3,1

0,00*

ATCC 33020/[7]

AFE_3150

coxB

cytochrome c oxidase, aa 3-type, subunit II

2,9

0,00*

ATCC 33020/[7]

AFE_3149

coxA

cytochrome c oxidase, aa 3-type, subunit I

2,5

0,00*

ATCC 33020/[7]

AFE_3148

coxC

cytochrome c oxidase, aa 3-type, subunit III

1,7

0,02*

ATCC 33020/[7]

AFE_3146

rus

rusticyanin

1,6

0,00*

ATCC 33020/[7]

CCM 4253/[24]

ATCC 19859/[25]

AFE_3146

rus

rusticyanin

2,1

0,00*

ATCC 33020/[7]

CCM 4253/[24]

ATCC 19859/[25]

AFE_3146

rus

rusticyanin

1,9

0,00*

ATCC 33020/[7]

CCM 4253/[24]

ATCC 19859/[25]

Cytochrome c oxidase complex biogenesis operon

AFE_3144

ctaA

heme A synthase

2,4

0,00*

 

AFE_3143

ctaB

heme O synthase, protoheme IX farnesyltransferase

0,4

0,18

 

AFE_3142

ctaT

major facilitator family transporter

0,8

0,00*

 

AFE_3141

ctaR

iron responsive regulator of the Rrf2 family

ND

ND

 

AFE_3139

ctaU

hypothetical protein

1,8

0,00*

 

AFE_3138

ctaS

oxidoreductase, 2OG-Fe(II) oxygenase family

1,4

0,00*

 

Sensor/regulator two-component signal transduction system

AFE_3137

regA

DNA-binding response regulator

0,7

0,02

 

AFE_3136

regB

sensor histidine kinase

2,0

0,00*

 

NADH complex operon

     

AFE_2630

nuoA

NADH-quinone oxidoreductase, A subunit

-0,1

0,80

 

AFE_2629

nuoB

NADH-quinone oxidoreductase, B subunit

0,3

0,13

 

AFE_2628

nuoC

NADH-quinone oxidoreductase, C subunit

0,0

0,69

 

AFE_2627

nuoD

NADH-quinone oxidoreductase, D subunit

-0,5

0,64

 

AFE_2626

nuoE

NADH-quinone oxidoreductase, E subunit

-1,1

0,05

 

AFE_2625

nuoF

NADH-quinone oxidoreductase, F subunit

0,4

0,01

 

AFE_2624

nuoG

NADH-quinone oxidoreductase, G subunit

-0,2

0,24

 

AFE_2623

nuoH

NADH-quinone oxidoreductase, H subunit

-0,7

0,00

 

AFE_2622

nuoI

NADH-quinone oxidoreductase, I subunit

1,4

0,00*

 

AFE_2621

nuoJ

NADH-quinone oxidoreductase, J subunit

-0,1

0,21

 

AFE_2620

nuoK

NADH-quinone oxidoreductase, K subunit

0,9

0,03*

 

AFE_2619

nuoL

NADH-quinone oxidoreductase, L subunit

-0,9

0,00

 

AFE_2618

nuoM

NADH-quinone oxidoreductase, M subunit

-0,8

0,00

 

AFE_2617

nuoN

NADH-quinone oxidoreductase, N subunit

-0,4

0,19

 

ATP synthetase complex operon

AFE_3209

atpB

ATP synthase F0, A subunit

1,8

0,00*

 

AFE_3208

atpE

ATP synthase F0, C subunit

1,4

0,00*

 

AFE_3207

atpF

ATP synthase F0, B subunit

0,4

0,01

 

AFE_3206

atpH

ATP synthase F1, delta subunit

0,4

0,02

 

AFE_3205

atpA

ATP synthase F1, alpha subunit

-0,2

0,99

 

AFE_3204

atpG

ATP synthase F1, gamma subunit

-0,5

0,23

 

AFE_3203

atpD

ATP synthase F1, beta subunit

0,0

0,75

 

AFE_3202

atpC

ATP synthase F1, epsilon subunit

-0,6

0,01

 
  1. Gene expression values (log2 ratio of median) for all genes/operons alluded in the revised model of Fe(II) oxidation in A. ferrooxidans ATCC 23270. Genes with a log2 ratio of median larger than |1.5| (corresponding to genes induced more than 2.8 fold) are considered differentially expressed (indicated with *) and genes p-value <0,005 are considered significant. Gene ID is that of Genbank genome annotation NC 011761. The reference and the strain in which the level of the gene product has been shown to be higher in Fe(II) than in S0 conditions are indicated in the last column. ND: not determined.