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U3 snoRNA genes are multi-copy and frequently linked to U5 snRNA genes in Euglena gracilis§

BMC Genomics volume 10, Article number: 528 (2009) Cite this article

Abstract

Background

U3 snoRNA is a box C/D small nucleolar RNA (snoRNA) involved in the processing events that liberate 18S rRNA from the ribosomal RNA precursor (pre-rRNA). Although U3 snoRNA is present in all eukaryotic organisms, most investigations of it have focused on fungi (particularly yeasts), animals and plants. Relatively little is known about U3 snoRNA and its gene(s) in the phylogenetically broad assemblage of protists (mostly unicellular eukaryotes). In the euglenozoon Euglena gracilis, a distant relative of the kinetoplastid protozoa, Southern analysis had previously revealed at least 13 bands hybridizing with U3 snoRNA, suggesting the existence of multiple copies of U3 snoRNA genes.

Results

Through screening of a λ genomic library and PCR amplification, we recovered 14 U3 snoRNA gene variants, defined by sequence heterogeneities that are mostly located in the U3 3'-stem-loop domain. We identified three different genomic arrangements of Euglena U3 snoRNA genes: i) stand-alone, ii) linked to tRNAArg genes, and iii) linked to a U5 snRNA gene. In arrangement ii), the U3 snoRNA gene is positioned upstream of two identical tRNAArg genes that are convergently transcribed relative to the U3 gene. This scenario is reminiscent of a U3 snoRNA-tRNA gene linkage previously described in trypanosomatids. We document here twelve different U3 snoRNA-U5 snRNA gene arrangements in Euglena; in each case, the U3 gene is linked to a downstream and convergently oriented U5 gene, with the intergenic region differing in length and sequence among the variants.

Conclusion

The multiple U3 snoRNA-U5 snRNA gene linkages, which cluster into distinct families based on sequence similarities within the intergenic spacer, presumably arose by genome, chromosome, and/or locus duplications. We discuss possible reasons for the existence of the unusually large number of U3 snoRNA genes in the Euglena genome. Variability in the signal intensities of the multiple Southern hybridization bands raises the possibility that Euglena contains a naturally aneuploid chromosome complement.

Background

Euglena gracilis is a free-living protist, distantly related to kinetoplastid protozoons [1], and whose RNA molecular biology displays a number of peculiar features. One example is the cytoplasmic ribosome of Euglena and the genes encoding its constituent rRNAs. Typically in eukaryotes, hundreds to thousands of copies of the rRNA operon are tandemly encoded on linear chromosomes. In Euglena, a single copy of the rRNA operon is carried on an 11-kbp extrachromosomal circular plasmid that is present in high copy number (estimates range between 800 and 4000 per cell) and that replicates autonomously, with few if any integrated chromosomal rRNA genes [2–5]. Furthermore, a number of additional, novel internal transcribed spacer sequences in the Euglena 28S rDNA are excised during pre-rRNA processing [6]. Consequently, the mature LSU rRNA, which typically consists of two stable species (5.8S plus 28S rRNA), is composed of 14 rRNA pieces (5.8S plus 13 28S 'fragments') in Euglena [7]. A similar, but less extensive pre-rRNA processing in trypanosomes yields a seven-fragment LSU rRNA [8–10]. Despite the extreme fragmentation of the Euglena LSU rRNA, the individual pieces are able to associate in trans [11] to form functional ribosomes [12–15]. Furthermore, ongoing mapping of O2'-methylribose and pseudouridine positions (M.N. Schnare & M.W. Gray, unpublished) suggests that the constituent LSU rRNA is the most highly modified rRNA of any organism examined to date [16].

The s mall n ucleo lar RNAs (snoRNAs) constitute a very large family of small RNAs present in the nucleolus as ribonucleoprotein complexes. Most snoRNAs guide the site-specific formation of O2'-methylribose or pseudouridine modifications in rRNAs and snRNAs [17–19]. Some of the processing and cleavage events required for the liberation of mature rRNAs from the pre-rRNA transcript are mediated by a subset of snoRNAs. One such processing snoRNA, the box C/D snoRNA U3, was the first snoRNA identified [20] and has since become the most extensively studied.

U3 snoRNA consists of 5'- and 3'-domains separated by a hinge region [21]. The 5'-domain contains sequence elements that are complementary to regions of the 5'-external transcribed spacer (ETS) of the pre-rRNA, as well as to the 5'-end of the 18S rRNA [22–26]. Base-pairing interactions between the pre-rRNA and its complementary regions in U3 snoRNA guide, by a complex and incompletely understood mechanism that includes the participation of U14 [27, 28] and U17/snR30 [29–31] snoRNAs, the multiple sequence-specific pre-rRNA cleavage events that eventually liberate the mature 5'-end of the 18S rRNA [22–26, 32]. Additional base-pairing interactions occur between the central hinge region of U3 snoRNA and the 5'-ETS of the pre-rRNA [5, 33–35]. These multiple base-pairing interactions may also confer on U3 snoRNA a chaperone-like activity in the co-transcriptional folding of the 18S rRNA [25]. The 3'-domain of U3 snoRNA, important in protein binding and RNA stability, contains box C/D sequence elements characteristic of this snoRNA family [21, 24].

U3 snoRNA associates with at least 43 proteins (the four box C/D snoRNA proteins plus 25 U-t hree-associated p roteins (Utps) and 14 others) to form a large ribonucleoprotein complex termed the SSU processome [21, 36]. In 'Miller chromatin spreads' of actively transcribed rRNA genes visualized by electron microscopy, this massive 80S complex of ~2.2 MDa corresponds to the terminal knobs observed on the 5'-ends of growing pre-rRNA 'Christmas trees' [36, 37].

Information about the many players, such as U3 snoRNA and its protein components, and the multiple, intricate and highly coordinated events in rRNA processing and ribosome biogenesis, has accumulated largely from studies in the phylogenetically narrow grouping of animals and yeasts. Therefore, our current understanding of ribosome biogenesis may not be truly representative of the many potentially different strategies used by phylogenetically disparate organisms. For this reason, the idiosyncratic features of RNA biology in E. gracilis coupled with its key phylogenetic placement make this organism a potentially informative alternative model system for the study of rRNA maturation, ribosome biogenesis and the many RNA and protein components that participate in this highly complex process.

Euglena U3 snoRNA [38] is a 180-nt species exhibiting the sequence motifs typical of U3 snoRNAs [21]. While considerably smaller than its homologues in yeast and vertebrates (333 nt, S. cerevisiae [21]; 217 nt, human [39]), Euglena U3 snoRNA is comparable in size to its trypanosomatid relatives (143 nt, T. brucei [40]). Unexpectedly, whereas U3 snoRNA is encoded by a single-copy gene in all examined trypanosome species [40–42], heterogeneities observed in RNA sequencing along with multiple hybridizing bands in Southern analysis [38] strongly suggested that U3 snoRNA is a multi-copy gene in the Euglena genome. In trypanosomatids, the genomic neighborhood of the U3 snoRNA locus is particularly rich in genes for other small RNAs, such as tRNAs and snRNAs [41–43]. Furthermore, in these organisms, the expression of the U3 snoRNA gene is dependent upon the presence of a closely linked, upstream and divergently oriented tRNA gene [44, 45]. Thus, we reasoned that an exploration of the genomic contexts of the Euglena U3 snoRNA gene loci would uncover both commonalities and differences with respect to the trypanosomatid scenario. In addition, insights might be gained both into the evolution of U3 snoRNA and the functional architecture of the Euglena genome.

Here, we describe the results of a comprehensive analysis of E. gracilis U3 snoRNA genes and their genomic contexts. We confirm that unlike the trypanosomatid case, where U3 snoRNA is a single-copy gene, the Euglena U3 snoRNA is encoded by a multigene family comprising at least 14 members. As in trypanosomes, most Euglena U3 snoRNA genes are located near genes for other small RNAs, such as tRNAs and U5 snRNA. However, notable differences between the Euglena and the trypanosomatid arrangements are evident, the evolutionary and functional implications of which are considered here.

Results

Multiple Southern hybridizing bands imply that the gene encoding U3 snoRNA is multi-copy in the Euglena genome

A complex and reproducible pattern of multiple hybridizing bands is produced in Southern hybridization experiments with a Euglena U3 snoRNA gene probe (Fig. 1A), implying that this gene is present in multiple copies in the Euglena genome. The resulting hybridization patterns show at least 13 hybridizing Eco R1, Bam H1 or Eco R1/Bam H1 fragments, ranging in size from ~1.8 to ~38.5 kbp.

Figure 1
figure 1

Southern analysis of restriction endonuclease-digested Euglena DNA demonstrating multiple bands hybridizing with a U3 snoRNA gene probe. (A) Euglena DNA was digested with Bam H1 (B), Eco R1 (E) or with both (B/E) endonucleases, two restriction enzymes that do not have recognition sequences within the Euglena U3 snoRNA gene. Restriction fragments were resolved by electrophoresis prior to Southern transfer and hybridization with a Euglena U3 snoRNA probe, all as described in Methods. The exact number of hybridizing fragments in the 10 to 38.5 kbp size range is difficult to determine owing to the limited resolution of this size range on standard agarose gels. (MW; a mixture of Invitrogen™ λ DNA/Hin dIII, 1 Kb Plus and λ DNA/High Molecular Weight Markers) (B) Densitometric analysis confirms that the multiple hybridizing bands display unequal hybridization signal intensities. Euglena DNA, digested with Eco R1 (E), was resolved by electrophoresis in a 0.7% agarose/1× TAE gel prior to Southern transfer and hybridization. The results of densitometric analysis using ImageJ [97] are shown to the right of the sample lane, with peak areas corresponding to the relative signal intensities of the labeled bands. For the indicated bands, the first number gives the area under the peak while the second number represents signal intensity normalized to that of the 2.5-kbp band (signal intensity 1425, the lowest in the lane).

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Unexpectedly, the multiple U3-hybridizing fragments showed reproducible differences in signal intensity (Fig. 1B), with this apparent non-stoichiometry being confirmed by densitometric analysis. Comparison within each sample lane revealed a number of bands with very similar signal intensities, including the 2.5-, 3.1-, and 9.2-kbp bands (signal intensities 1425, 1479, and 2138, respectively; Fig. 1B). Bands at 1.8, 5.5, and 12.2 kbp also displayed very similar signal intensities (3890, 4431, and 4202, respectively). On the other hand, differences in relative signal intensity within a sample lane are clearly illustrated by comparison of the 1.7-, 1.8- and 2.2-kbp bands, whose signal intensities were 1.9×, 2.7×, and 3.9× that of the 2.5-kbp band (2743, 3890 and 5502, respectively, vs.1425). Similar results have been obtained with other hybridization probes (see below). The reproducibility of these results suggests that they are not attributable to incomplete restriction endonuclease digestion of the DNA or to uneven transfer of restriction fragments from gel to membrane prior to hybridization.

Three different U3 snoRNA gene arrangements identified in a λ library of Euglena genomic DNA

To investigate further the genomic organization of Euglena U3 snoRNA, we screened a λ library of Euglena genomic DNA using a Euglena U3 snoRNA gene probe, retrieving several U3-hybridizing λ clones. Sequence walking in conjunction with BLASTn analysis revealed four unique U3 snoRNA gene variants in three different genomic contexts (Fig. 2A-C): i) a stand-alone gene, ii) linkage to tRNA genes, and iii) linkage to a U5 snRNA gene.

Figure 2
figure 2

Three different U3 snoRNA gene arrangements in λ clones of Euglena genomic DNA. Clone maps of (A) λ U3-Solitary (total size ~12 kbp; 4824 bp sequenced; U3-containing portion 2515 bp), (B) λ U3-tRNAArg (total size ~14 kbp; 6096 bp sequenced; U3-containing portion 2868 bp) and (C1 and C2) λ U3-U5 (total sizes 1772 and 516 bp, respectively) are shown. Terminal sequences of specified length (bp) do not contain any features of interest. Sizes of the intergenic spacers are indicated by the numbers between the various genes. Red lines demarcate the positions of probes used in Southern hybridization experiments. Repetitive elements, such as the Euglena Eg MS7 microsatellite [47] (filled rectangle), the U3 snoRNA pseudo-3'-end repeat (U3Ψ3' filled rectangle) and repetitive sequences also found within certain Euglena γ-tubulin introns (filled triangles) are indicated. Solid diamonds denote simple sequence repeats that precluded effective primer design for additional primer walking. Open diamonds with letters denote homopolymer runs (A, C or G) that prematurely terminated sequencing. Due to the technical challenges posed by the many repetitive sequence elements, the λ U3-Solitary and λ U3-tRNAArg clones were not sequenced in their entirety; dashed lines denote unsequenced portions. In the λ U3-U5-C2 clone, U3* refers to a 5' truncation of the U3 gene as a result of cloning. Clone maps not drawn to scale.

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A solitary U3 snoRNA gene arrangement was identified in a large 12-kbp genomic fragment (Fig. 2A). No additional, recognizable genes or sequence elements were found by BLASTn analysis.

A U3-hybridizing λ clone was found to encode a U3 snoRNA gene neighbored downstream by two identical arginine tRNA genes, both encoded in the opposite transcriptional orientation relative to the U3 snoRNA gene (Fig. 2B). The coding regions of the U3 snoRNA gene and first tRNAArg gene are separated by 209 bp, whereas the two tRNAArg genes are 70 bp apart. No additional genes were found by BLASTn analysis. This clone contains many short simple-sequence elements, also identified in other λ clones, such as homopolymer and dinucleotide repeats. In addition, unusual repetitive sequence elements are present, such as repeat sequences that are also present within the introns of the Euglena γ-tubulin gene paralogs [46]. The region upstream of the U3 snoRNA gene also contains a Euglena microsatellite sequence, Eg MS7 [47]. Finally, a 52-nt region, consisting of the 3'-end and putative transcription termination sequence of this particular U3 snoRNA gene variant, has evidently been duplicated. The resulting sequence consists of a full-length U3 snoRNA gene and transcription termination signal followed by the 3'-terminal 22-nt of the U3 snoRNA gene (nt 159 to 180) and a nearly identical copy of the transcription termination signal.

The identified tRNAArg gene is unremarkable in sequence and secondary structure (Fig. 3A). It adopts a conventional clover-leaf secondary structure, displays the expected D and TΨC loops, and possesses a small loop in the variable region.

Figure 3
figure 3

(A) The Euglena tRNAArg adopts a conventional clover-leaf secondary structure. The anticodon nucleotides, UCG, are highlighted in red. Watson-Crick base-pairing interactions are depicted as dashes (-) and G/U pairings with dots (•). (B) The Euglena U5 snRNA displays conventional secondary structure features. The phylogenetically conserved and functionally essential exon-interacting nucleotides (green) of loop I (gray background) and the Sm binding site (blue) are highlighted. Nucleotide heterogeneities and an indel in the gene variants are indicated in red.

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Two different U3-hybridizing λ clones were identified in which the U3 snoRNA gene is neighbored downstream by a convergently oriented U5 snRNA gene (Fig. 2C1/C2). No additional genes were detected. While the U3 snoRNA and U5 snRNA sequences in the two clones are highly similar, the size (255 and 160 bp) and sequence of the U3 snoRNA-U5 snRNA intergenic spacers are very different (see below). Furthermore, the sequence of the regions downstream of the U5 snRNA genes is equally dissimilar. As with the other λ clones, the two U3-U5 genomic inserts contain many short repetitive sequence elements and stretches of extreme nucleotide bias (e.g., hompolymeric stretches of up to 12 C or G residues).

As with most protist U snRNAs, the Euglena U5 snRNA gene sequence was previously unknown. Its nucleotide sequence and secondary structure (Fig. 3B) display features present in U5 snRNAs from other organisms. The Euglena U5 snRNA is 98 nt in length, the position of its 5'-end inferred by comparison with other U5 snRNA sequences. The precise 3'-end was determined by 3' RACE analysis and by chemical sequencing of the RNA (data not shown). The secondary structure consists, in its 5'-region, of a stem-loop region punctuated by a central bulge. The 11-nt terminal loop I contains the invariant 9-nt sequence (5'-GCCUUUUAC-3') known to interact with exon sequences at the 5'- and 3'-splice sites [48]. The 3'-region contains a conventional Sm binding site. Notably, a small stem-loop structure, typically present near the 3'-end of U5 snRNAs, is not found in the Euglena U5 snRNA.

Southern analysis suggests that Euglena U3 snoRNA genes are frequently linked to U5 snRNA genes

Although intensive screening of the Euglena λ genomic library identified only four different U3 snoRNA genes in three distinct genomic contexts, Southern analysis of Euglena genomic DNA revealed at least 13 U3-hybridizing bands. Because we could not account for many U3 snoRNA genes (and their genomic arrangements), Southern analysis was performed to determine whether additional variants of the linkages identified in the λ genomic fragments are present in the Euglena genome.

Southern analysis of Euglena genomic DNA using a tRNAArg gene probe identified multiple hybridizing bands (12 in Bam HI/Eco RI, ranging in size from 2.1 kbp to 16 kbp; Fig. 4A), suggesting that the tRNAArg gene is also multi-copy in the Euglena genome. This result was not unexpected, considering that tRNA genes frequently constitute large, multigene families.

Figure 4
figure 4

Southern analysis of Euglena DNA hydrolyzed with Bam H1 + Eco RI (BE) reveals few U3-tRNAArg but multiple U3-U5 gene linkages. (A) Hybridization with probes corresponding to the Euglena U3 and tRNAArg genes, and to a region upstream of the U3 gene (UpStr U3) but containing the Euglena microsatellite Eg MS7 [47] sequence, all as described in Methods. MW: a mixture of Invitrogen™ λ DNA/Hin dIII, 1 Kb Plus and λ DNA/High Molecular Weight Markers. (B) Hybridization with a Euglena U5 probe. (C) Probes corresponding to the IGS sequence of the U3-U5 linkage families A and B (both sub-families B1 and B2) confirm the genomic PCR results. Bands co-hybridizing with U3, U5 and IGS probes are indicated by the asterisks. The IGS probes frequently hybridized to large restriction fragments and gave low signal intensities, despite long autoradiographic exposures. Accordingly, in the case of the IGS lanes only, the contrast was uniformly adjusted over the entire lane to aid in the identification of hybridizing band(s).

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A single band, co-hybridizing with the U3 and tRNAArg probes (indicated by the asterisks in Fig. 4), is suggestive of a single U3-tRNAArg gene linkage in the Euglena genome. Other members of the tRNAArg gene family do not appear to be similarly linked to U3 snoRNA genes.

The authenticity of the apparent U3-tRNAArg co-hybridization was further substantiated by the observation that a probe derived from the region upstream of the U3 gene in the U3-tRNAArg λ clone (Fig. 2B) predominantly labeled the band that hybridized with both the U3 and tRNAArg probes (•, Fig. 4A). This probe also contains the Euglena microsatellite sequence [47] mentioned above, which likely explains the relatively high level of background hybridization seen in this particular case.

Southern analysis of Euglena genomic DNA with a U5 gene probe identified ~14 hybridizing fragments, ranging in size from 0.9 kbp to 13 kbp. (Fig. 4B). Thus, U5 snRNA is also encoded by multiple genes in the Euglena genome.

Comparison of the U5 Southern hybridization result with the U3 one revealed at least eight co-migrating hybridization bands (asterisks, Fig. 4B). Thus, the majority of U5 snRNA genes, though not all, appeared to be linked to U3 snoRNA genes in the Euglena genome.

In addition, as observed with the U3-hybridizing bands, the U5-hybridizing bands also showed reproducible differences in hybridization intensity. Furthermore, the relative signal intensities within the U5 pattern co-vary with those within the U3 pattern.

Genomic PCR confirms multiple U3 snoRNA-U5 snRNA gene linkages in the Euglena genome

To examine putative U3-U5 gene linkages in detail, we used a genomic PCR strategy to amplify, clone and sequence U3-U5 intergenic spacers (IGS) as well as the flanking U3 and U5 genes. As expected from the sequence of the two U3-U5 λ clones and from the results of Southern analysis, multiple PCR products were obtained (Fig. 5A). To ensure complete coverage of all U3-U5 linkages, a total of 122 clones, from five different PCR-generated libraries, were sequenced and analyzed, with multiple clones of each linkage being identified. Thus, nucleotide heterogeneities between the different U3-U5 linkages are considered legitimate and not attributable to Taq-induced errors or to PCR-mediated recombination [49–51].

Figure 5
figure 5

Multiple amplification products from Euglena genomic PCR reveal U3 snoRNA-U5 snRNA gene linkages that cluster into related families and sub-families. (A) PCR amplification of total Euglena DNA with primers designed to the 5'-regions of the convergently oriented U3 snoRNA and U5 snRNA genes (oEg U3-F1 and oEg U5-F1; see Additional file 2) yields multiple amplification products ranging in size from ~425 to ~550 bp. (B) Alignment of PCR products demonstrates high sequence conservation of the U3 snoRNA and U5 snRNA genes, both within a linkage family and between linkage families. Numbers at the ends of the alignments indicate the percent sequence identity (% ID) among family members within the IGS, excluding the U3 and U5 coding sequences and putative transcription termination signals. Family members that are identical within the IGS may nevertheless display nucleotide heterogeneities within the flanking U3 or U5 genes. The alignment begins at nt 10 of the U3 snoRNA gene, owing to the location of the PCR primer.

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The cloned U3-U5 PCR products ranged in size from 422 to 541 bp. Detailed sequence analysis identified a total of 12 unique U3-U5 linkages. Comparison of the unique sequences revealed that the multiple U3 snoRNA sequences are highly similar, as are the multiple U5 snRNA sequences, the members of each group displaying only limited sequence heterogeneities (Fig. 5B). However, substantial variability is seen in the size and sequence of the IGS separating the U3 and U5 genes. Despite this variability, regions of sequence similarity within the IGS suggest that the gene linkages may be related (e.g., compare the IGS in A1a and A1b, Fig. 5B). Thus, the 12 unique U3 snoRNA-U5 snRNA gene linkages appear to form five families that can be further divided into sub-families (Fig. 5B).

U3-U5 family A, representing the linkage having the longest IGS sequence, comprises four members. Based on sequence similarity, the members of this family can be divided into two sub-families, A1 and A2, with the A1 subfamily being further divided into A1a, A1b and A1c. Members of the A family show the highest level of identity, exhibiting only minor nucleotide changes and a 17-nt indel in the IGS sequence. The A1a PCR linkage (Fig. 5B) corresponds to the U3-U5 linkage identified in the λ genomic clone C1 (Fig. 2).

The B-linkage family contains two members, B1 and B2. The IGS sequences of the two linkages show the lowest level of sequence identity of any of the linkage families. The relatedness of the members of the B-linkage family is based on the presence of short regions of sequence identity, which are punctuated by regions of nucleotide divergence. Short regions of apparent sequence similarity at both ends of the IGSs, immediately downstream of the 3'-ends of the U3 and U5 genes, may correspond to transcription termination signals, in which case these regions do not actually contribute to linkage family relatedness. The B1 PCR linkage (Fig. 5B) corresponds to the U3-U5 linkage identified in the λ genomic clone C2 (Fig. 2).

The C-, D-, and E-linkage families each contain two members, C1 and C2, D1 and D2, and E1 and E2. Each linkage family exhibits regions of sequence similarity interrupted by regions of nucleotide difference and by indels. Overall, the level of sequence identity in the members of the C, D and E linkage families is intermediate between that of the highly similar A family and the very divergent B family. Some of the U3-U5 linkages were confirmed by Southern hybridization analysis (Fig. 4C).

A similar genomic PCR approach was used to search for other arrangements of linked U3-U5 genes, such as divergently (head-to-head) and similarly (tail-to-head) oriented genes. The possible presence of tandem U3 snoRNA genes was also investigated. No linkages of these types were found.

A revised secondary structure model for Euglena U3 snoRNA

The nucleotide heterogeneities identified in the U3 variants prompted a re-assessment of the likely secondary structure of Euglena U3 snoRNA. A recent phylogenetic comparison of U3 sequences from representative taxa [21] has uncovered additional conserved sequence elements and RNA motifs that are also present in the Euglena U3 snoRNA (Fig. 6). An alignment of known U3 snoRNA sequences from representative organisms is shown in Additional file 1. Furthermore, information from mutational studies [21, 33] and from in vivo chemical and enzymatic structure-probing experiments [24, 52] has resulted in significant revision of the proposed general secondary structure of U3 snoRNA. Accordingly, a revised conserved sequence element and secondary structure model for Euglena U3 snoRNA is presented here (Fig. 6).

Figure 6
figure 6

Revised secondary structure model of the Euglena U3 snoRNA. In this proposed model, the 5'-trimethylguanosine cap (5'-TMG cap) is indicated in orange, the phylogenetically conserved and functionally essential box GAC, A', A, C', B, C, and D elements are shown in blue. Pseudouridine (Ψ) residues are indicated in green. Nucleotide heterogeneities identified in the Euglena U3 snoRNA gene variants are denoted by the red circles. Nucleotides forming a K-turn motif are highlighted on a gray background. Conventional Watson-Crick base-pairing interactions are depicted as dashes (-) and G/U pairings are identified by dots (•). An alignment of known U3 snoRNA sequences from representative organisms is shown in Additional file 1.

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Sequence elements in the 5' region of U3 are known to interact with the 5' external transcribed spacer (5'-ETS) of the pre-rRNA and with the 5' region of the 18S rRNA. The first such element in U3 snoRNA is the GAC box, followed by the A' box and then the A box [21]. In the identified Euglena U3 genes, a single nucleotide heterogeneity has been mapped to this region. The heterogeneity, an A-to-G transition, is located downstream of the A box sequence.

The central hinge region of the Euglena U3 snoRNA contains a small stem-loop structure consisting of a 3-bp stem with a 5-nt loop. As in other organisms [22], potential base-pairing interactions may occur between the Euglena U3 hinge domain (both the 5' and 3' regions) and the 5' external transcribed spacer (5' ETS) of the pre-rRNA [5]. In the multiple Euglena U3 genes, a sequence heterogeneity is located in the 3' hinge region. This A-to-C transversion is located immediately downstream of the small stem-loop structure.

The 3'-terminal domain of the Euglena U3 snoRNA contains conserved sequence elements, boxes C', B, C and D, known to interact with RNA-binding proteins [53, 54]. This domain consists of an extended stem-loop structure punctuated by a number of bulge-loop elements. In our revised model, box C' and box D elements are juxtaposed in the secondary structure context, as are box B and box C elements. The box elements are mainly single-stranded, corresponding to small bulge-loop structures; however, some regions may form short base-pairing interactions. More importantly, alternative base-pairing interactions in the 3'-terminal domain allow for the formation of two kink-turn (K-turn) RNA motifs [54–56] within the juxtaposed box C'/D and B/C elements.

Nearly all of the sequence heterogeneities identified in the multiple Euglena U3 snoRNA variants (12 of the 14) are located in the 3'-extended stem-loop domain. Ten of the 12 sequence heterogeneities are transitions, vs. two transversions. The majority of nucleotide heterogeneities in helical regions result in shifts from Watson-Crick base-pairings (U-A and G-C) to non-canonical interactions (three U•G, one G•U). Three heterogeneities occur in single-stranded regions, none of which is part of a single-stranded conserved box element. Two nucleotide heterogeneities, present in the same U3 snoRNA variant near the terminal stem-loop structure, form compensatory base changes (C-G to U-A) and thus maintain base-pairing interactions. Two additional nucleotide heterogeneities map to the terminal base pairs of short stem regions, immediately adjacent to the opening or closing of bulge-loop structures. Since neither of these heterogeneities maintains the terminal base-pairing interactions of the stems, a slight expansion of the adjacent bulge-loop structures results. One of the two heterogeneities is located within the box C' element, adjacent to the 5+2 motif of the K-turn [55, 56]. Only one heterogeneity, located in a short stem region, disrupts a standard base-pairing interaction. In sum, the distribution of the 12 sequence heterogeneities identified in the 3'-extended stem-loop domain of Euglena U3 snoRNA results in minimal changes to the overall secondary structure of the region.

Discussion

In Euglenozoa, U3 snoRNA genes are frequently linked to genes for other small RNAs

In one of three patterns of U3 snoRNA gene organization identified here, a U3 snoRNA gene is linked to two identical, downstream and convergently oriented (relative to the U3 snoRNA gene) tRNAArg genes in the Euglena genome (Fig. 2B). Close linkage of U3 snoRNA and tRNA genes has been found in a number of trypanosomatids, members of the kinetoplastid protozoa, which are the closest relatives of the euglenids. The most dramatic example is in Leishmania tarentolae, where a 2.7-kbp genomic region encodes the U3 snoRNA gene along with genes for 10 tRNAs, U1 snRNA and 5S RNA (Fig. 7) [41]. Sequence for a portion of the corresponding region in L. major (GenBank AQ843909) reveals the same gene content organized in the same manner. In both Leishmania cases, the U3 snoRNA gene is neighbored by a single, downstream, convergently oriented tRNAArg gene (possessing the same anticodon, UCG, as in the Euglena case).

Figure 7
figure 7

Organization of U3 snoRNA genes within Euglenozoa. The U3 snoRNA genes are shown in red, U5 snRNA gene in green, tRNA genes in blue and all other genes are shown in black. Euglena gracilis A, B and C correspond to the arrangements depicted in Fig. 2. Numbers shown between the various genes correspond to the sizes of the intergenic spacers separating the two genes. Numbers on the right hand of the figure indicate the size (bp) of the respective genomic region. Note that this figure is not drawn to scale. Organisms represented include Euglena gracilis (this study), Leishmania tarentolae [GenBank:L20948], Trypanosoma brucei [GenBank:X57047] and Leptomonas collosoma [GenBank:L32919].

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In Trypanosoma brucei, the U3 snoRNA gene is neighbored upstream by a divergently oriented tRNAArg gene (having a different anticodon, ACG); no proximal genes are encoded downstream of the T. brucei U3 snoRNA gene (Fig. 7) [42, 57]. In Leptomonas collosoma, a divergently oriented tRNALys gene is also encoded upstream of the U3 snoRNA gene [43] (no sequence is available downstream). Similarly, an upstream, divergently oriented tRNALys gene neighbors the U3 snoRNA gene in both L. tarentolae and L. major, in addition to the downstream, convergently oriented tRNAArg gene noted above. In all of these trypanosomatid cases, the IGS separating the U3 and tRNA genes is 71 to 106 bp in size, compared to 209 bp in the Euglena. In the phylogenetically unrelated ciliate Tetrahymena thermophila, a U3 snoRNA gene is also neighbored by an upstream, identically oriented tRNALys gene. However, in this case, the U3-tRNA IGS is nearly 500 bp in size [58]. Evidently, close physical linkage of U3 snoRNA and tRNA genes is a widespread phenomenon within the trypanosomatids, with similar linkages in Euglena and in Tetrahymena.

The most striking observation to emerge from the present study is the multiplicity of distinct U3 snoRNA genes and gene arrangements in the Euglena genome, in stark contrast to what is seen in trypanosomatids [40–43]. In addition to the U3-tRNAArg linkage, we document here 12 distinct examples of U3 snoRNA-U5 snRNA gene linkage in the Euglena genome. In all these examples, the U5 snRNA gene is located downstream and in the opposite transcriptional orientation relative to the U3 snoRNA gene (Fig. 2 and 5). To date, no linked U3-U5 genes have been identified in other organisms. Furthermore, with the exception of tRNA genes, U3 snoRNA genes have not been found directly linked to other genes encoding small RNAs.

Genes specifying modification-guide box C/D and box H/ACA snoRNAs are frequently clustered, including in trypanosomatids [59, 60] and in Euglena [61]. So far, however, U3 snoRNA genes have not been found linked to modification-guide snoRNA genes in any eukaryote, and this also appears to be the case in Euglena.

Influence of U3 snoRNA gene organization on U3 snoRNA gene expression

The expression of U3 snoRNA genes in trypanosomatids and other eukaryotes has been extensively studied. In all instances examined, trypanosomatid [62], other protist [58] and plant [63, 64] U3 snoRNA genes are transcribed by RNA polymerase III (RNAP III). Based on the sister-group relationship between kinetoplastids and euglenids [1], U3 snoRNA is similarly assumed to be synthesized by RNAP III in Euglena, although this inference remains to be confirmed experimentally. In all cases, U3 snoRNA genes are transcribed from their own promoter and U3 transcripts do not appear to be part of larger, polycistronic transcripts.

In trypanosomatids, the expression of U3 snoRNA is dependent on the linked, upstream, divergently oriented tRNA gene. Two extragenic regulatory elements (A and B boxes) required for trypanosome U3 snoRNA gene expression [44] correspond to the tRNA D and TΨC loops, which are known to serve generally as intragenic RNAP III promoter elements for tRNA genes. Furthermore, the spacing of the A and B boxes relative to each other and to the U3 snoRNA gene is critical. As noted above, the distance between the upstream tRNA gene and the U3 snoRNA gene varies within a narrow range (93-105 bp) in trypanosomatids (Fig. 7). Transcription factor TFIIIC is known to bind to the B box element of the tRNA gene, whereupon it recruits transcription factor TFIIIB, which in turn sequesters RNAP III for transcription initiation [65, 66] In yeast, transcription of tRNA genes by RNAP III prevents nucleosome assembly in the immediate vicinity of these genes, and concomitant nucleosome-mediated repression [67]. Thus, the binding of TFIIIC to the B box is postulated to play an indirect role, through chromatin remodeling, in the expression of trypanosomatid tRNA-linked U3 snoRNA genes [44, 62]. Whether the downstream tRNAArg genes play a similar role in U3 snoRNA transcription in Euglena remains to be determined. The Euglena U3 snoRNA-tRNA genes are not as closely linked (209 bp IGS) as they are in trypanosomatids (93-105 bp IGS). However, the presence of two tRNAArg genes in the Euglena case might result in more efficient recruitment of TFIIIC to this site and a parallel chromatin remodeling effect over a larger region. The fact remains that most U3 snoRNA genes in Euglena are evidently not linked to tRNA genes, so any parallel with U3 snoRNA gene expression in trypanosomatids must necessarily be limited.

Gene duplication in the generation of multiple U3 snoRNA-U5 snRNA gene linkages in Euglena

The 12 different U3 snoRNA-U5 snRNA gene linkages described here cluster into five discrete families, based on sequence conservation within the IGS (Fig. 5B). In the 'A' family, three linkage variants form a sub-family (A1a-A1c) while a fourth linkage is the sole member of the second sub-family (A2). Linkage families B, C, D, and E contain two U3 snoRNA-U5 snRNA gene linkages each. No regions of sequence conservation common to all 12 linkage variants are evident within the IGS.

At this point, the origin and generation of the multiple U3 snoRNA-U5 snRNA gene linkages remains a matter of conjecture. It is not unreasonable to suppose that the current arrangement arose via duplication of one or a few ancestral U3 snoRNA-U5 snRNA gene linkages. The pathway of formation and fixation of the ancestral U3-U5 gene linkage(s) is unclear, but the linkage per se presumably was generated by random gene shuffling and perhaps maintained by a favorable effect on gene expression. Multiple rounds of locus, chromosome and/or genome duplication followed by sequence divergence likely then created the observed linkage families and sub-families.

In support of this proposal, accumulating evidence from sequence analysis indicates that parts of the Euglena genome are highly repeated and that the genome may be evolutionarily plastic. An arrangement similar to the U3-U5 linkage case has been described for the Euglena spliced leader RNA (SL RNA) gene, which is linked to a 5S rRNA gene [68]. As with the U5 snRNA gene, some SL RNA genes are dispersed throughout the genome; however, the majority are linked to an identically oriented 5S rRNA gene. The linkage is encoded within a tandemly repeated 0.6-kbp unit, and it is estimated that up to 300 SL RNA-5S rRNA gene linkages are present in the Euglena genome. In the absence of large-scale linkage data, we do not know whether the U3 snoRNA-U5 snRNA gene linkages described here might be similarly present in the Euglena genome as repeated arrays, although PCR amplification results would seem to rule out any closely linked U3-U5 units.

Limited information on gene copy number for other Euglena snRNAs gives a contrasting picture. Southern hybridization analysis of Euglena DNA with a Euglena U1 snRNA [69] gene probe yields a single hybridizing band, a result substantiated by PCR amplification and the sequencing of several independent Euglena U1 snRNA clones, which revealed no nucleotide heterogeneities (unpublished results). In contrast, when 3' RACE-based PCR amplification was used to obtain the sequence of the Euglena U4 snRNA [70], at least four sequence variants were obtained (unpublished results). Additional evidence for the repetitive nature of portions of the Euglena genome has come from studies of Euglena modification-guide (box C/D and box H/ACA) snoRNAs, where many cases of multi-copy, linked snoRNA genes have been found [16, 61]. Similarly, on-going bioinformatic screens of Euglena cDNAs have revealed multiple allelic variants of typically single-copy ribosomal protein genes (unpublished results). Thus, it appears that many, though not all, genes are multi-copy in the genome of Euglena.

Are U3 snoRNA genes spatially organized in the nucleus?

A recent model for the spatial organization of RNAP III-transcribed genes in the nucleus may be relevant to the organization and expression of U3 snoRNA genes in Euglena, trypanosomatids and some other organisms. This model stems from in situ hybridization studies suggesting that the 274 tRNA genes of yeast, although dispersed throughout the linear map of the genome, are localized to the nucleolus [71, 72]. This situation is analogous to the three-dimensional co-localization of the multiple rDNA genes in the nucleolus. Accordingly, it has been proposed that chromosomal loci encoding tRNA genes also associate in three-dimensional space within the nucleus. Such an arrangement could conceivably lead to the formation of a tRNA transcription and processing center enriched in RNAP III, transcription, and processing factors [71, 73]. Experimental evidence suggests that U3 snoRNA genes in human cells may associate in three-dimensional space with coiled bodies in the nucleus [39, 74]. Thus, the three-dimensional clustering of genes transcribed by RNAP III could promote the formation of 'transcription territories' [75] that function to more efficiently recruit transcription complexes to the region, thereby maintaining a high level of gene expression. Although it is not known whether this scenario applies to Euglena U3 snoRNA genes, the suggestion is a plausible one considering that Euglena U3 snoRNA genes are likely transcribed by RNAP III and for the most part are linked either to tRNA genes or to the RNAP III-transcribed U5 snRNA gene.

Why so many U3 snoRNA genes in the Euglena genome?

The Euglena genome encodes at least 14 different U3 snoRNA genes. While U3 snoRNA is an essential gene, it is unclear why the Euglena genome would encode so many copies, considering that the U3 snoRNA gene is single-copy in trypanosomatid protozoa [40–43]. At least part of the explanation for this evident expansion of U3 snoRNA genes in Euglena may have to do with the unusual features of its rRNA genes and mature rRNA species.

In a Euglena cell, a single unit of linked rRNA genes is encoded on each of ~1000-4000 copies of an extrachromosomal plasmid-like DNA species [2–5], in contrast to the arrangement of chromosomally integrated tandem rDNA arrays in most other eukaryotes. Electron micrographs show an unusual, extensively segmented nucleolar structure in Euglena, which might reflect the organization of the individual plasmid-like rDNA elements into a number of distinct, higher order clusters within the nucleus. (In Entamoeba sp., the rDNA is similarly encoded on an extrachromosomal DNA element that is able to induce formation of a dispersed nucleolar structure located at the nuclear periphery [76].) If U3 snoRNA genes are also physically and functionally localized at or near the nucleolus, the additional numbers of U3 snoRNA genes seen in Euglena may be required to accommodate an atypical nucleolar organization of its rDNA genes, thereby more efficiently coordinating U3 snoRNA expression and function with rRNA synthesis and processing.

Another consideration is that, relative to the situation in a typical eukaryote, many additional spacer sequences are removed during pre-rRNA processing in Euglena, resulting in a naturally and highly fragmented 28S rRNA equivalent [6, 7]. One suggestion is that Euglena U3 snoRNA might participate in these additional pre-rRNA processing events [38]. Alternatively, the additional processing steps might result in the production of a relatively higher proportion of defective ribosomes than in other eukaryotes, in which case a relatively larger number of pre-rRNA transcripts would presumably need to be processed in order to maintain an adequate number of functional ribosomes in the cell. This requirement would in turn necessitate a greater number of U3 snoRNA molecules, a requirement that presumably could be achieved by encoding and expressing an expanded number of U3 snoRNA genes.

A problem with the above suggestions is that trypanosomatid LSU rRNAs are also fragmented, although not as extensively as those in Euglena [8–10]. Nevertheless, as noted above, the U3 snoRNA gene is single copy in trypanosomatid genomes [40–43]. Thus, it appears unlikely that the multi-copy nature of Euglena U3 snoRNA genes could be a direct consequence of the particularities of rRNA processing in Euglena.

U3 snoRNA genes and the Euglena genome

Considering the long history of Euglena gracilis as a 'laboratory workhorse', surprisingly little is known about its genome. The varying intensities of U3 snoRNA-hybridizing bands in Southern blots of Euglena DNA suggests that the actual number of U3 snoRNA genes may be substantially higher than the 14 we have documented here. Technical considerations in restriction endonuclease digestion, transfer of restriction fragments and subsequent hybridization have been eliminated as possible sources of artifact contributing to hybridization variability, based on the reproducibility of the results under different experimental conditions.

Aneuploidy, in which a cell contains different numbers of one or more chromosomes, appears to be the most likely explanation for the varying intensities of hybridizing bands. In many organisms, aneuploidy is associated with genome instability, as in human Trisomy-21 (Down's syndrome) and many cancers. However, aneuploidy has been postulated to occur normally in a number of protists [77] and in fungi [77–79], although in these cases it does not appear to result in genome instability.

Aneuploidy has been reported in a number of trypanosomatids, including Trypanosoma cruzi [77, 80–83] and Leishmania [84]. In such cases, results similar to those presented here have been obtained. In pulsed field gel electrophoretic analysis of T. cruzi DNA, the ethidium bromide staining intensities of different chromosomal bands varies within single samples. Furthermore, DNA-content variability of up to 70% has been observed in cells derived from a single clone. There is as yet no direct evidence for aneuploidy in Euglena; however, the demonstrated occurrence of aneuploidy in trypanosomatids, in conjunction with the results presented here, makes aneuploidy a distinct possibility in Euglena.

Conclusion

The comprehensive analysis reported here has revealed multiple U3 snoRNA genes in the protist Euglena gracilis, in three distinct genomic arrangements: i) stand-alone, ii) linked to two tandem, identical tRNAArg genes, and iii) linked to a U5 snRNA gene. The multiple U3 snoRNA-U5 snRNA gene linkages, which cluster into distinct families based on sequence similarities within the intergenic spacer, presumably arose by genome, chromosome, and/or locus duplications. We suggest that the evident expansion of U3 snoRNA genes in Euglena, compared to its kinetoplastid distant relatives, may have to do with the unusual features of Euglena rRNA genes and mature rRNA species and/or a highly recombinogenic genome. In view of the variability in the signal intensities of the multiple bands consistently observed in our Southern hybridization experiments, we further raise the possibility that Euglena contains a naturally aneuploid chromosome complement.

Methods

Extraction of total DNA from Euglena gracilis

A streptomycin-bleached, aplastidic variant of Euglena gracilis [2], derived from the UCLA variety of E. gracilis strain Z, was grown in 1-liter cultures of medium [85] at room temperature with gentle agitation. The medium was modified by addition of ethanol to 30 mM as a carbon source [2], CoCl2 (1.3 mg/l) and Na2MoO4 (0.2 mg/l) in place of Co(NO3)2 and H2MoO4, respectively, and adjusted to pH 6.5 with phosphoric acid [86]. Cultures were harvested at mid- to late-log phase (after 4-5 days of growth), at an OD600 between 0.8 and 1.0.

Total Euglena DNA was prepared using a modified detergent/chloroform/phenol extraction procedure. Euglena cultures, held on ice for 30 min, were centrifuged at 3,520 g for 20 min at 4°C. The cells were subsequently washed in a total of 150 ml cold Extraction Wash Buffer (EWB = 25 mM EDTA-Tris (pH 8.5): an EDTA solution titrated to pH 8.5 with solid Tris base), pooled, recovered by centrifugation at 3,520 g for 10 min and resuspended to a final volume of 10 ml with room temperature EWB.

Cells were lysed by the addition of 2.5 ml 25% SDS. The solution was gently mixed until homogeneous, after which the lysate volume was adjusted to 25 ml with room temperature EWB. To the lysate, 4 ml 8 M sodium perchlorate was added and the solution was gently mixed to homogeneity. Nucleic acids were extracted twice with chloroform:isoamyl alcohol (24:1) and precipitated with an equal volume of room temperature 2-propanol. High-molecular-weight DNA was preferentially recovered by spooling onto a glass rod, then washed with 80% ethanol, briefly dried and dissolved in 10 ml TE (10 mM Tris-HCl (pH 7.6), 0.1 mM EDTA). Once re-dissolved, the DNA was further deproteinized by phenol-cresol extraction until no material was visible at the interface. The DNA was precipitated from the aqueous phase with ethanol, washed, briefly dried and dissolved in 1.0 ml TE.

Contaminating RNA in the DNA preparation was removed by RNase treatment. DNA was preferentially recovered by polyethylene glycol (PEG) precipitation [87], which involved adjusting the solution to final concentrations of 0.5 M NaCl and 10% PEG and incubating on ice for 30 min. The PEG-precipitated DNA was recovered by centrifugation at 11,180 g for 15 min at 4°C. The DNA was washed twice with 80% ethanol, briefly dried and redissolved in TE. Residual PEG was removed by additional phenol extractions and ethanol precipitations.

DNA amplification by polymerase chain reaction

PCR amplifications (50 μl reactions) consisted of 200 μM of each dNTP, 1× ThermoPol buffer (New England BioLabs), 2.5 U Thermus aquaticus DNA polymerase, 20 pmol each oligonucleotide primer [see Additional file 2] and 100-500 ng total Euglena DNA. In certain cases, amplifications were improved by the use of sheared genomic DNA (50 passes through a 29-gauge syringe). Thermal cycling conditions consisted of 96°C for 5 min followed by 35 cycles of i) 95°C for 40 sec, ii) 55°C for 40 sec and iii) 72°C for 50 sec with a final extension at 72°C for 7 min. PCR parameters for products intended for cloning in the TOPO TA Cloning® system (Invitrogen™) consisted of 30 amplification cycles and a final extension at 72°C for 30 min, as recommended by the manufacturer.

Purification of DNA fragments by gel electrophoresis

Small DNA fragments (<1 kbp) were resolved by electrophoresis in 1-2.5% (w/v) low-melting-point (LMP) agarose gels containing 1× TAE (100 mM Tris, 0.1 mM Na2•EDTA, titrated to pH 8.0 with glacial acetic acid). Gel slices (0.4-0.55 g) were transferred to 2-ml microcentrifuge tubes. The slices were crushed then melted by incubation at 65°C for 30 min. The melted gel was incubated with 0.5 vol. phenol-cresol, pre-warmed at 65°C, and incubated for 5 min at 65°C with frequent vigorous agitation. The supernatant was recovered by centrifugation and extracted twice more with phenol. After the addition of 0.1 vol. 3 M NaOAc, the supernatant was extracted four additional times with phenol at room temperature, or until no material was visible at the interface. DNA was precipitated with ethanol (facilitated by the addition of 5 μl 0.25% linear polyacrylamide carrier [88]), recovered by centrifugation, washed in 75% ethanol, dried and redissolved in 5-10 μl TE.

Cloning of PCR products, plasmid minipreps and sequence analysis

When necessary, PCR products were purified by gel electrophoresis using the LMP agarose/hot phenol method, or with the Sephaglas™ BandPrep kit (Amersham Biosciences), and cloned using the TOPO TA Cloning® kit (Invitrogen™). Plasmid DNA was prepared using the QIAprep® Spin Miniprep kit (Qiagen) and sequenced in-house. Large insert clones were sequenced by primer walking [see Additional file 2]. Sequence chromatograms were analyzed and contig assembly was performed using the Staden Package software suite [89]. Gene sequences were identified by BLAST [90] sequence similarity searches at GenBank http://www.ncbi.nlm.nih.gov/blast/. Sequence alignments were generated using ClustalX [91] and manually edited with BioEdit [92]. RNA secondary structures were drawn with XRNA [93].

Southern hybridization analysis

Total Euglena DNA was digested with the specified restriction endonuclease (10 U/μg DNA, in the presence of DTT and BSA) for 5 hr at 37°C, to ensure complete digestion. For these experiments, we used a combination of Bam H1 and Eco R1, two restriction endonucleases that do not have recognition sequences within the Euglena U3, U5 or tRNAArg genes or in the U3-U5 IGS sequences examined. Restriction fragments, 10-13 μg/lane, were resolved by electrophoresis in a 0.5% agarose/1× TAE gel at 1 V/cm for 20 hr. DNA fragments were depurinated and denatured [94, 95] prior to capillary transfer in 0.4 M NaOH to a charged nylon membrane (GeneScreen Plus®, NEN® Life Science Products) according to the manufacturer's alkaline transfer protocol. Following overnight transfer, the membrane was neutralized in 0.2 M Tris-HCl (pH 7.6)/2× SSC (1× SSC = 150 mM NaCl, 15 mM sodium citrate) for 5 min, washed in 2× SSC for 5 min and baked at 80°C for ~8 hr. The nylon membrane was cut into strips corresponding to groups of duplicate sample lanes.

Cloned Southern hybridization probes were either excised from their plasmid vector by restriction digestion or amplified by PCR from the plasmid insert, with LMP agarose/hot phenol-based gel purification when necessary. Approximately 100 ng of each probe was labeled by DNA synthesis in the presence of random hexamer primers [96] and [α-32P]dATP.

Membranes were soaked in 6× SSC for 5 min, then washed for >30 min in 6× SSC at hybridization temperature (42°C). The blots were pre-hybridized for 4-18 hr in Southern hybridization solution (5× Denhardt's solution, 5× SSC, 25 mM Na2HPO4, 25 mM NaH2PO4, 180 μg/ml sheared and denatured herring testes DNA, 50% formamide, 1% SDS) [38, 95], then hybridized for 18-22 hr. The membranes were washed at 42°C by five 10-min incubations in 2× SSC/0.1% SDS and once or twice for 15 min in 0.1× SSC/0.1% SDS. The relative signal intensities of the multiple hybridizing bands were quantified by densitometric analysis using ImageJ [97] to determine the area under each peak.

Screening of an E. gracilis λ Genomic DNA Library

An E. gracilis λ genomic DNA library, constructed in the λBlueSTAR™ vector (Novagen), was screened by plaque lift (as per the membrane manufacturer's protocol; Magna Lift, Osmonics) and hybridization with a Euglena U3 snoRNA gene probe. Hybridization and washing conditions were identical to those described for Southern hybridization. Positive λ plaques were excised in vivo into plasmid clones by Cre-mediated recombination and transferred to E. coli DH5α cells, as described in the library kit protocol. Plasmid DNAs were prepared and sequenced as described above.

Accession Numbers

The Euglena sequences presented here have been deposited under the following accession numbers: λ U3 snoRNA-solitary [GenBank:GQ338155, GenBank:GU080027 and GenBank:GU080026]; λ U3 snoRNA-tRNAArg [GenBank:GU080028, GenBank:GU080030 and GenBank:GU080029]; λ U3 snoRNA-U5 snRNA C1 [GenBank:GU080031]; λ U3-U5 C2 [GenBank:GU080032]; U3 snoRNA-U5 snRNA linkage family A1a, contained within λ genomic clone C1 [GenBank:GU080031]; U3-U5 linkage A1b [GenBank:GU080033]; U3-U5 linkage A1c [GenBank:GU080034]; U3-U5 linkage A2 [GenBank:GU080035]; U3-U5 linkage B1, contained within λ genomic clone C2 [GenBank:GU080032]; U3-U5 linkage B2 [GenBank:GU080036]; U3-U5 linkage C1 [GenBank:GU080037]; U3-U5 linkage C2 [GenBank:GU080038]; U3-U5 linkage D1 [GenBank:GU080039]; U3-U5 linkage D2 [GenBank:GU080040]; U3-U5 linkage E1 [GenBank:GU080041]; U3-U5 linkage E2 [GenBank:GU080042].

Note

§ This paper is dedicated to the memories of Claude Charette, the father of JMC, and Grazyna Tokarczyk, a technician in the lab, both of whom passed away while this work was in progress.

References

  1. Keeling PJ, Burger G, Durnford DG, Lang BF, Lee RW, Pearlman RE, Roger AJ, Gray MW: The tree of eukaryotes. Trends Ecol Evol. 2005, 20: 670-676. 10.1016/j.tree.2005.09.005.

    Article  PubMed  Google Scholar 

  2. Cook JR, Roxby R: Physical properties of a plasmid-like DNA from Euglena gracilis. Biochim Biophys Acta. 1985, 824: 80-83.

    Article  CAS  PubMed  Google Scholar 

  3. Ravel-Chapuis P, Nicolas P, Nigon V, Neyret O, Freyssinet G: Extrachromosomal circular nuclear rDNA in Euglena gracilis. Nucleic Acids Res. 1985, 13: 7529-7537. 10.1093/nar/13.20.7529.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  4. Ravel-Chapuis P: Nuclear rDNA in Euglena gracilis: paucity of chromosomal units and replication of extrachromosomal units. Nucleic Acids Res. 1985, 16: 4801-4810. 10.1093/nar/16.11.4801.

    Article  Google Scholar 

  5. Greenwood SJ, Schnare MN, Cook JR, Gray MW: Analysis of intergenic spacer transcripts suggests 'read-around' transcription of the extrachromosomal circular rDNA in Euglena gracilis. Nucleic Acids Res. 2001, 29: 2191-2198. 10.1093/nar/29.10.2191.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  6. Schnare MN, Cook JR, Gray MW: Fourteen internal transcribed spacers in the circular ribosomal DNA of Euglena gracilis. J Mol Biol. 1990, 215: 85-91. 10.1016/S0022-2836(05)80097-X.

    Article  CAS  PubMed  Google Scholar 

  7. Schnare MN, Gray MW: Sixteen discrete RNA components in the cytoplasmic ribosome of Euglena gracilis. J Mol Biol. 1990, 215: 73-83. 10.1016/S0022-2836(05)80096-8.

    Article  CAS  PubMed  Google Scholar 

  8. White TC, Rudenko G, Borst P: Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to Domain VII of other eukaryotic 28S rRNAs. Nucleic Acids Res. 1986, 14: 9471-9489. 10.1093/nar/14.23.9471.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  9. Campbell DA, Kubo K, Clark CG, Boothroyd JC: Precise identification of cleavage sites involved in the unusual processing of trypanosome ribosomal RNA. J Mol Biol. 1987, 196: 113-124. 10.1016/0022-2836(87)90514-6.

    Article  CAS  PubMed  Google Scholar 

  10. Spencer DF, Collings JC, Schnare MN, Gray MW: Multiple spacer sequences in the nuclear large subunit ribosomal RNA gene of Crithidia fasciculata. EMBO J. 1987, 6: 1063-1071.

    PubMed Central  CAS  PubMed  Google Scholar 

  11. Smallman DS, Schnare MN, Gray MW: RNA:RNA interactions in the large subunit ribosomal RNA of Euglena gracilis. Biochim Biophys Acta. 1996, 1305: 1-6.

    Article  PubMed  Google Scholar 

  12. Eisenstadt JM, Brawerman G: The protein-synthesizing systems from the cytoplasm and the chloroplasts of Euglena gracilis. J Mol Biol. 1964, 10: 392-402. 10.1016/S0022-2836(64)80060-7.

    Article  CAS  PubMed  Google Scholar 

  13. Eisenstadt JM, Brawerman G: Characteristics of a cell-free system from Euglena gracilis for the incorporation of amino acids into protein. Biochim Biophys Acta. 1964, 80: 463-472.

    CAS  PubMed  Google Scholar 

  14. Avadhani NG, Buetow DE: Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis. Biochem J. 1972, 128: 353-365.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  15. Avadhani NG, Buetow DE: Mitochondrial and cytoplasmic ribosomes. Distinguishing characteristics and a requirement for the homologous ribosomal salt-extractable fraction for protein synthesis. Biochem J. 1974, 140: 73-78.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  16. Russell AG, Schnare MN, Gray MW: A large collection of compact box C/D snoRNAs and their isoforms in Euglena gracilis: structural, functional and evolutionary insights. J Mol Biol. 2006, 357: 1548-1565. 10.1016/j.jmb.2006.01.063.

    Article  CAS  PubMed  Google Scholar 

  17. Meier UT: The many facets of H/ACA ribonucleoproteins. Chromosoma. 2005, 114: 1-14. 10.1007/s00412-005-0333-9.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  18. Matera AG, Terns RM, Terns MP: Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs. Nat Rev Mol Cell Biol. 2007, 8: 209-220. 10.1038/nrm2124.

    Article  CAS  PubMed  Google Scholar 

  19. Reichow SL, Hamma T, Ferré-D'Amaré AR, Varani G: The structure and function of small nucleolar ribonucleoproteins. Nucleic Acids Res. 2007, 35: 1452-1464. 10.1093/nar/gkl1172.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  20. Hodnett JL, Busch H: Isolation and characterization of uridylic acid-rich 7 S ribonucleic acid of rat liver nuclei. J Biol Chem. 1968, 243: 6334-6342.

    CAS  PubMed  Google Scholar 

  21. Samarsky DA, Fournier MJ: Functional mapping of the U3 small nucleolar RNA from the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1998, 18: 3431-3444.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  22. Beltrame M, Tollervey D: Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J. 1995, 14: 4350-4356.

    PubMed Central  CAS  PubMed  Google Scholar 

  23. Hughes JMX: Functional base-pairing interaction between highly conserved elements of U3 small nucleolar RNA and the small ribosomal subunit RNA. J Mol Biol. 1996, 259: 645-654. 10.1006/jmbi.1996.0346.

    Article  CAS  PubMed  Google Scholar 

  24. Méreau A, Fournier R, Grégoire A, Mougin A, Fabrizio P, Lührmann R, Branlant C: An in vivo and in vitro structure-function analysis of the Saccharomyces cerevisiae U3A snoRNP: protein-RNA contacts and base-pair interaction with the pre-ribosomal RNA. J Mol Biol. 1997, 273: 552-571. 10.1006/jmbi.1997.1320.

    Article  PubMed  Google Scholar 

  25. Sharma K, Tollervey D: Base pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing. Mol Cell Biol. 1999, 19: 6012-6019.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  26. Borovjagin AV, Gerbi SA: Xenopus U3 snoRNA GAC-Box A' and Box A sequences play distinct functional roles in rRNA processing. Mol Cell Biol. 2001, 21: 6210-6221. 10.1128/MCB.21.18.6210-6221.2001.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  27. Liang W-Q, Fournier MJ: U14 base-pairs with 18S rRNA: a novel snoRNA interaction required for rRNA processing. Genes Dev. 1995, 9: 2433-2443. 10.1101/gad.9.19.2433.

    Article  CAS  PubMed  Google Scholar 

  28. Morrissey JP, Tollervey D: U14 small nucleolar RNA makes multiple contacts with the pre-ribosomal RNA. Chromosoma. 1997, 105: 515-522. 10.1007/BF02510488.

    Article  CAS  PubMed  Google Scholar 

  29. Enright CA, Maxwell ES, Eliceiri GL, Sollner-Webb B: 5'ETS rRNA processing facilitated by four small RNAs: U14, E3, U17, and U3. RNA. 1996, 2: 1094-1099.

    PubMed Central  CAS  PubMed  Google Scholar 

  30. Atzorn V, Fragapane P, Kiss T: U17/snR30 is a ubiquitous snoRNA with two conserved sequence motifs essential for 18S rRNA production. Mol Cell Biol. 2004, 24: 1769-1778. 10.1128/MCB.24.4.1769-1778.2004.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  31. Fayet-Lebaron E, Atzorn V, Henry Y, Kiss T: 18S rRNA processing requires base pairings of snR30 H/ACA snoRNA to eukaryote-specific 18S sequences. EMBO J. 2009, 28: 1260-1270. 10.1038/emboj.2009.79.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  32. Kass S, Tyc K, Steitz JA, Sollner-Webb B: The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing. Cell. 1990, 60: 897-908. 10.1016/0092-8674(90)90338-F.

    Article  CAS  PubMed  Google Scholar 

  33. Borovjagin AV, Gerbi SA: The spacing between functional cis-elements of U3 snoRNA is critical for rRNA processing. J Mol Biol. 2000, 300: 57-74. 10.1006/jmbi.2000.3798.

    Article  CAS  PubMed  Google Scholar 

  34. Borovjagin AV, Gerbi SA: Xenopus U3 snoRNA docks on pre-rRNA through a novel base-pairing interaction. RNA. 2004, 10: 942-953. 10.1261/rna.5256704.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  35. Borovjagin AV, Gerbi SA: An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA. Nucleic Acids Res. 2005, 33: 4995-5005. 10.1093/nar/gki815.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  36. Dragon F, Gallagher JEG, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, Wormsley S, Settlage RE, Shabanowitz J, Osheim Y, Beyer AL, Hunt DF, Baserga SJ: A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 2002, 417: 967-970. 10.1038/nature00769.

    Article  CAS  PubMed  Google Scholar 

  37. Osheim YN, French SL, Keck KM, Champion EA, Spasov K, Dragon F, Baserga SJ, Beyer AL: Pre-18S ribosomal RNA is structurally compacted into the SSU processome prior to being cleaved from nascent transcripts in Saccharomyces cerevisiae. Mol Cell. 2004, 16: 943-954. 10.1016/j.molcel.2004.11.031.

    Article  CAS  PubMed  Google Scholar 

  38. Greenwood SJ, Schnare MN, Gray MW: Molecular characterization of U3 small nucleolar RNA from the early diverging protist, Euglena gracilis. Curr Genet. 1996, 30: 338-346. 10.1007/s002940050142. [https://commerce.metapress.com/content/0bfgknnqmnk0x6el/resource-secured/?target=fulltext.pdf&sid=4q0t2o45cb5kca45maa0ed55&sh=www.springerlink.com]

    Article  CAS  PubMed  Google Scholar 

  39. Gao L, Frey MR, Matera AG: Human genes encoding U3 snRNA associate with coiled bodies in interphase cells and are clustered on chromosome 17p11.2 in a complex inverted repeat structure. Nucleic Acids Res. 1997, 25: 740-4747. 10.1093/nar/25.23.4740.

    Article  Google Scholar 

  40. Mottram J, Perry KL, Lizardi PM, Lührmann R, Agabian N, Nelson RG: Isolation and sequence of four small nuclear U RNA genes of Trypanosoma brucei subsp. brucei: identification of the U2, U4, and U6 RNA analogs. Mol Cell Biol. 1989, 9: 1212-1223.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  41. Shi X, Chen D-HT, Suyama Y: A nuclear tRNA gene cluster in the protozoan Leishmania tarentolae and differential distribution of nuclear-encoded tRNAs between the cytosol and mitochondria. Mol Biochem Parasitol. 1994, 65: 23-37. 10.1016/0166-6851(94)90112-0.

    Article  CAS  PubMed  Google Scholar 

  42. Mottram JC, Bell SD, Nelson RG, Barry JD: tRNAs of Trypanosoma brucei. Unusual gene organization and mitochondrial importation. J Biol Chem. 1991, 266: 18313-18317.

    CAS  PubMed  Google Scholar 

  43. Hartshorne T, Agabian N: A common core structure for U3 small nucleolar RNAs. Nucleic Acids Res. 1994, 22: 3354-3364. 10.1093/nar/22.16.3354.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  44. Nakaar V, Dare AO, Hong D, Ullu E, Tschudi C: Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes. Mol Cell Biol. 1994, 14: 6736-6742.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  45. Nakaar V, Tschudi C, Ullu E: An unusual liaison: Small nuclear and cytoplasmic RNA genes team up with tRNA genes in trypanosomatid protozoa. Parasitol Today. 1995, 11: 225-228. 10.1016/0169-4758(95)80085-9.

    Article  CAS  Google Scholar 

  46. Canaday J, Tessier LH, Imbault P, Paulus F: Analysis of Euglena gracilis alpha-, beta- and gamma-tubulin genes: introns and pre-mRNA maturation. Mol Genet Genomics. 2001, 265: 153-160. 10.1007/s004380000403.

    Article  CAS  PubMed  Google Scholar 

  47. Zhang W-J, Yang J, Yu Y-H, Shen Y-F: Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis. J Eukaryot Microbiol. 2005, 52: 356-359. 10.1111/j.1550-7408.2005.00044.x.

    Article  CAS  PubMed  Google Scholar 

  48. Newman AJ: The role of U5 snRNP in pre-mRNA splicing. EMBO J. 1997, 16: 5797-5800. 10.1093/emboj/16.19.5797.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  49. Judo MS, Wedel AB, Wilson C: Stimulation and suppression of PCR-mediated recombination. Nucleic Acids Res. 1998, 26: 1819-1825. 10.1093/nar/26.7.1819.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  50. Bradley RD, Hillis DM: Recombinant DNA sequences generated by PCR amplification. Mol Biol Evol. 1997, 14: 592-593.

    Article  CAS  PubMed  Google Scholar 

  51. Meyerhans A, Vartanian J-P, Wain-Hobson S: DNA recombination during PCR. Nucleic Acids Res. 1990, 18: 1687-1691. 10.1093/nar/18.7.1687.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  52. Antal M, Mougin A, Kis M, Boros E, Steger G, Jakab G, Solymosy F, Branlant C: Molecular characterization at the RNA and gene levels of U3 snoRNA from a unicellular green alga, Chlamydomonas reinhardtii. Nucleic Acids Res. 2000, 28: 2959-2968. 10.1093/nar/28.15.2959.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  53. Cléry A, Senty-Ségault V, Leclerc F, Raué HA, Branlant C: Analysis of sequence and structural features that identify the B/C motif of U3 small nucleolar RNA as the recognition site for the Snu13p-Rrp9p protein pair. Mol Cell Biol. 2007, 27: 1191-1206. 10.1128/MCB.01287-06.

    PubMed Central  Article  PubMed  Google Scholar 

  54. Marmier-Gourrier N, Cléry A, Senty-Ségault V, Charpentier B, Schlotter F, Leclerc F, Fournier R, Branlant C: A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA. RNA. 2003, 9: 821-838. 10.1261/rna.2130503.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  55. Klein DJ, Schmeing TM, Moore PB, Steitz TA: The kink-turn: a new RNA secondary structure motif. EMBO J. 2001, 20: 4214-4221. 10.1093/emboj/20.15.4214.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  56. Vidovic I, Nottrott S, Hartmuth K, Lührmann R, Ficner R: Crystal structure of the spliceosomal 15.5 kD protein bound to a U4 snRNA fragment. Mol Cell. 2000, 6: 1331-1342. 10.1016/S1097-2765(00)00131-3.

    Article  CAS  PubMed  Google Scholar 

  57. Marchetti MA, Tschudi C, Silva E, Ullu E: Physical and transcriptional analysis of the Trypanosoma brucei genome reveals a typical eukaryotic arrangement with close interspersion of RNA polymerase II- and III-transcribed genes. Nucleic Acids Res. 1998, 26: 3591-3598. 10.1093/nar/26.15.3591.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  58. Ørum H, Nielsen H, Engberg J: Structural organization of the genes encoding the small nuclear RNAs U1 to U6 of Tetrahymena thermophila is very similar to that of plant small nuclear RNA genes. J Mol Biol. 1992, 227: 114-121. 10.1016/0022-2836(92)90686-E.

    Article  PubMed  Google Scholar 

  59. Uliel S, Liang X-h, Unger R, Michaeli S: Small nucleolar RNAs that guide modification in trypanosomatids: repertoire, targets, genome organisation, and unique functions. Int J Parasitol. 2004, 34: 445-454. 10.1016/j.ijpara.2003.10.014.

    Article  CAS  PubMed  Google Scholar 

  60. Liang X-h, Ochaion A, Xu Y-x, Liu Q, Michaeli S: Small nucleolar RNA clusters in trypanosomatid Leptomonas collosoma. Genome organization, expression studies, and the potential role of sequences present upstream from the first repeated cluster. J Biol Chem. 2004, 279: 5100-5109. 10.1074/jbc.M308264200.

    Article  CAS  PubMed  Google Scholar 

  61. Russell AG, Schnare MN, Gray MW: Pseudouridine-guide RNAs and other Cbf5p-associated RNAs in Euglena gracilis. RNA. 2004, 10: 1034-1046. 10.1261/rna.7300804.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  62. Fantoni A, Dare AO, Tschudi C: RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements. Mol Cell Biol. 1994, 14: 2021-2028.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  63. Kiss T, Marshallsay C, Filipowicz W: Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro. Cell. 1991, 65: 517-526. 10.1016/0092-8674(91)90469-F.

    Article  CAS  PubMed  Google Scholar 

  64. Marshallsay C, Connelly S, Filipowicz W: Characterization of the U3 and U6 snRNA genes from wheat: U3 snRNA genes in monocot plants are transcribed by RNA polymerase III. Plant Mol Biol. 1992, 19: 973-983. 10.1007/BF00040529.

    Article  CAS  PubMed  Google Scholar 

  65. Geiduschek EP, Kassavetis GA: The RNA polymerase III transcription apparatus. J Mol Biol. 2001, 310: 1-26. 10.1006/jmbi.2001.4732.

    Article  CAS  PubMed  Google Scholar 

  66. Schramm L, Hernandez N: Recruitment of RNA polymerase III to its target promoters. Genes Dev. 2000, 16: 2593-2620. 10.1101/gad.1018902.

    Article  Google Scholar 

  67. Morse RH, Roth SY, Simpson RT: A transcriptionally active tRNA gene interferes with nucleosome positioning in vivo. Mol Cell Biol. 1992, 12: 4015-4025.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  68. Keller M, Tessier LH, Chan RL, Weil JH, Imbault P: In Euglena, spliced-leader RNA (SL-RNA) and 5S rRNA genes are tandemly repeated. Nucleic Acids Res. 1992, 20: 1711-1715. 10.1093/nar/20.7.1711.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  69. Breckenridge DG, Watanabe Y, Greenwood SJ, Gray MW, Schnare MN: U1 small nuclear RNA and spliceosomal introns in Euglena gracilis. Proc Natl Acad Sci USA. 1999, 96: 852-856. 10.1073/pnas.96.3.852.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  70. Russell AG, Watanabe Y, Charette JM, Gray MW: Unusual features of fibrillarin cDNA and gene structure in Euglena gracilis: evolutionary conservation of core proteins and structural predictions for methylation-guide box C/D snoRNPs throughout the domain Eucarya. Nucleic Acids Res. 2005, 33: 2781-2791. 10.1093/nar/gki574.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  71. Thompson M, Haeusler RA, Good PD, Engelke DR: Nucleolar clustering of dispersed tRNA genes. Science. 2003, 302: 1399-1401. 10.1126/science.1089814.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  72. Haeusler RA, Engelke DR: Spatial organization of transcription by RNA polymerase III. Nucleic Acids Res. 2006, 34: 4826-4836. 10.1093/nar/gkl656.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  73. Kosak ST, Groudine M: Gene order and dynamic domains. Science. 2004, 306: 644-647. 10.1126/science.1103864.

    Article  CAS  PubMed  Google Scholar 

  74. Schul W, Adelaar B, van Driel R, de Jong L: Coiled bodies are predisposed to a spatial association with genes that contain snoRNA sequences in their introns. J Cell Biochem. 1999, 75: 393-403. [http://www3.interscience.wiley.com/journal/66004419/abstract?CRETRY=1&SRETRY=0]

    Article  CAS  PubMed  Google Scholar 

  75. Oliver B, Misteli T: A non-random walk through the genome. Genome Biol. 2005, 6: 214-10.1186/gb-2005-6-4-214.

    PubMed Central  Article  PubMed  Google Scholar 

  76. Jhingan GD, Panigrahi SK, Bhattacharya A, Bhattacharya S: The nucleolus in Entamoeba histolytica and Entamoeba invadens is located at the nuclear periphery. Mol Biochem Parasitol. 2009, 167: 72-80. 10.1016/j.molbiopara.2009.04.011.

    Article  CAS  PubMed  Google Scholar 

  77. Kondrashov AS: Evolutionary genetics of life cycles. Annu Rev Ecol Syst. 1997, 28: 391-435. 10.1146/annurev.ecolsys.28.1.391.

    Article  Google Scholar 

  78. Carr J, Shearer GJ: Genome size, complexity, and ploidy of the pathogenic fungus Histoplasma capsulatum. J Bacteriol. 1998, 180: 6697-6703.

    PubMed Central  CAS  PubMed  Google Scholar 

  79. Pitt CW, Moreau E, Lunness PA, Doonan JH: The pot1 + homologue in Aspergillus nidulans is required for ordering mitotic events. J Cell Sci. 2004, 117: 199-209. 10.1242/jcs.00844.

    Article  CAS  PubMed  Google Scholar 

  80. Pérez-Castiñeira JR, Alvar J, Ruiz-Pérez LM, Serrano A: Evidence for a wide occurrence of proton-translocating pyrophosphatase genes in parasitic and free-living protozoa. Biochem Biophys Res Commun. 2002, 294: 567-573. 10.1016/S0006-291X(02)00517-X.

    Article  PubMed  Google Scholar 

  81. El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran A-N, Wortman JR, Alsmark UCM, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N: Comparative genomics of trypanosomatid parasitic protozoa. Science. 2005, 309: 404-409. 10.1126/science.1112181.

    Article  CAS  PubMed  Google Scholar 

  82. McDaniel JP, Dvorak JA: Identification, isolation, and characterization of naturally-occurring Trypanosoma cruzi variants. Mol Biochem Parasitol. 1993, 57: 213-222. 10.1016/0166-6851(93)90197-6.

    Article  CAS  PubMed  Google Scholar 

  83. Henriksson J, Åslund L, Pettersson U: Karyotype variability in Trypanosoma cruzi. Parasitol Today. 1996, 12: 108-114. 10.1016/0169-4758(96)80670-3.

    Article  CAS  PubMed  Google Scholar 

  84. Lighthall GK, Giannini SH: The chromosomes of Leishmania. Parasitol Today. 1992, 8: 192-199. 10.1016/0169-4758(92)90263-2.

    Article  CAS  PubMed  Google Scholar 

  85. Cramer M, Myers J: Growth and photosynthetic characteristics of Euglena gracilis. Arch Mikrobiol. 1952, 17: 384-402. 10.1007/BF00410835.

    Article  CAS  Google Scholar 

  86. Danilov RA, Ekelund NGA: Effects of pH on the growth rate, motility and photosynthesis in Euglena gracilis. Folia Microbiol. 2001, 46: 549-554. 10.1007/BF02818001.

    Article  CAS  Google Scholar 

  87. Lis JT, Schleif R: Size fractionation of double-stranded DNA by precipitation with polyethylene glycol. Nucleic Acids Res. 1975, 2: 383-389. 10.1093/nar/2.3.383.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  88. Gaillard C, Strauss F: Ethanol precipitation of DNA with linear polyacrylamide as carrier. Nucleic Acids Res. 1990, 18: 378-10.1093/nar/18.2.378.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  89. Staden R, Judge DP, Bonfield JK: Sequence assembly and finishing methods. Methods Biochem Anal. 2001, 43: 303-322. [http://www.bioon.com/book/biology/Bioinformatics/chapter-13.pdf]

    Article  CAS  PubMed  Google Scholar 

  90. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.

    Article  CAS  PubMed  Google Scholar 

  91. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25: 4876-4882. 10.1093/nar/25.24.4876.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  92. Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999, 41: 95-98.

    CAS  Google Scholar 

  93. Weiser B, Noller HF: XRNA: Auto-interactive program for modeling RNA. 1995, The Center for Molecular Biology of RNA, University of California, Santa Cruz. Santa Cruz, California, [http://rna.ucsc.edu/rnacenter/xrna/xrna.html]

    Google Scholar 

  94. Current protocols in molecular biology. Edited by: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl, K. 1989, John Wiley & Sons, Toronto, [http://mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc]

    Google Scholar 

  95. Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: a laboratory manual. 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  96. Feinberg AP, Vogelstein B: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983, 132: 6-13. 10.1016/0003-2697(83)90418-9.

    Article  CAS  PubMed  Google Scholar 

  97. Rasband WS: ImageJ. 2006, U.S. National Institutes of Health, Bethesda, Maryland, USA, [http://rsb.info.nih.gov/ij/index.html]

    Google Scholar 

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Acknowledgements

We are indebted to Drs. A J Lohan, MN Schnare and DF Spencer for invaluable advice, assistance and discussions; M Dlutek for automated DNA sequencing; and MN Schnare for generating RNA secondary structure diagrams and for chemical sequencing to confirm the structure of Euglena U5 snRNA. This work was supported by funding from the Canadian Institutes of Health Research to MWG (grant MOP-11212, which included an associated studentship to JMC) and salary support to MWG from the Canada Research Chairs Program and the Canadian Institute for Advanced Research.

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  1. Michael W Gray

    Present address: Room 8-F2, Sir Charles Tupper Medical Building, Dalhousie University, 5850 College Street, Halifax, B3H 1X5, Nova Scotia, Canada

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  1. Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada

    J Michael Charette & Michael W Gray

  2. Current address: Department of Molecular Biophysics & Biochemistry, Yale University School of Medicine, Sterling Hall of Medicine, 333 Cedar St, Room SHM C-114, PO Box 208024, New Haven, CT, 06520-8024, USA

    J Michael Charette

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Authors' contributions

JMC performed the experimental work, interpreted the results and drafted the manuscript. MWG conceived the project, provided advice and interpretation, and contributed to and revised the manuscript. Both authors read and approved the final manuscript.

Electronic supplementary material

12864_2009_2412_MOESM1_ESM.PDF

Additional file 1: U3 snoRNA sequence alignment. An alignment of known U3 snoRNA sequences from representative organisms. Conserved sequence features in U3 snoRNA, boxes GAC, A', A, C', B, C, and D are shown, along with regions of the alignment corresponding to the central hinge and 3'-hairpin domains. Representative organisms include: Arabidopsis thaliana [GenBank:X52629, nt 325-541], Chlamydomonas reinhardtii [GenBank:AJ001179, nt 171-392], Crithidia fasciculata [GenBank:AF277396], Dictyostelium discoideum [GenBank:V00190, nt 62-271], Euglena gracilis [GenBank:U27297], Homo sapiens [GenBank:M14061, nt 277-493], Leishmania major [GenBank:AQ843909], Leishmania tarentolae [GenBank:L20948, complement of nt 2128-1984], Leptomonas collosoma [GenBank:L32919, nt 391-533], Mus musculus [GenBank:X63743, nt 815-1027], Saccharomyces cerevisiae [GenBank:X05498], Schizosaccharomyces pombe [GenBank:X56982, nt 37-291], Tetrahymena thermophila [GenBank:X71349], Triticum aestivum [GenBank:X63065, nt 858-1063], Trypanosoma brucei [GenBank:M25776], Trypanosoma cruzi [GenBank:AAHK01001296, nt 4547-4689] and Xenopus laevis [GenBank:X07318, nt 1-219]. (PDF 2 MB)

12864_2009_2412_MOESM2_ESM.DOC

Additional file 2: DNA oligonucleotides. A list of DNA oligonucleotide primers used for PCR amplifications, DNA sequencing and 3' RACE. (DOC 80 KB)

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Charette, J.M., Gray, M.W. U3 snoRNA genes are multi-copy and frequently linked to U5 snRNA genes in Euglena gracilis§. BMC Genomics 10, 528 (2009). https://doi.org/10.1186/1471-2164-10-528

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Keywords

  • tRNA Gene
  • Sequence Heterogeneity
  • snoRNA Gene
  • External Transcribe Spacer
  • snRNA Gene

BMC Genomics

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