Source

Num df

Den df

F

Sig.

Partial R^{2}


Model

58

2248.56

134.76

<0.001

77.66

Total pool size (bp)

1

85.40

14.25

<0.001

14.30

Average sequence length

1

86.29

52.87

<0.001

37.99

GC content

1

86.60

34.89

<0.001

28.72

NonACGT content

1

84.49

0.05

0.823

<0.01

Enzyme combination

27

2428.48

199.26

<0.001

68.90

Enzyme combination*GC content

27

2428.48

112.38

<0.001

55.55

 Variance partitioning addressing the contribution of enzyme combination (28 combinations) on pool coverage for 92 eukaryotic species (see additional file 1). Species was included as a random factor in a mixed model analysis which aimed to determine the influence of individual factors or interactions (Source). cDNA pool coverage was weighted by the number of sequences per species to account for variation in available sequence data. The numerator and denominator (KenwardRoger corrected) degrees of freedom (Num df/Den df) are provided. F statistics (F) and the significance (Sig.) of the overall model, factors and interactions are reported. The proportion of the variation in cDNA pool coverage which is explained by each factor/interaction is indicated as Partial Rsquare values [27].