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Table 3 Reproducibility of expression of non-core FeHm stimulon genes

From: The iron/heme regulated genes of Haemophilus influenzae: comparative transcriptional profiling as a tool to define the species core modulon

  NTHi R2866 Hib 10810 Rd KW20
Locusa Arrayb QPCRc Arrayb QPCRc Arrayd QPCRc
HI0098 ns -1.56 -2.70 -2.80 -3.55 -4.28
HI0099 ns -2.69 -2.16 -2.48 -3.56 -3.82
HI0152 ns 1.47 -2.12 -1.44 -1.08 -1.13
HI0153m -2.70 1.10 -2.24 -2.02 not on -1.38
HI0164 ns 4.02 1.26 2.01 1.63 1.98
HI0223 ns -1.33 ns 1.10 -2.45 -4.70
HI0246 ns -1.75 -1.94 -4.29 ns -1.20
HI0254 ns -1.10 -2.56 -2.41 -1.43 -2.22
HI0298 ns 1.14 -1.97 -1.08 ns -1.25
HI0342 3.21 4.7 ns 2.20 not on 1.54
HI0361 -7.89 -3.14 ns -1.30 -8.91 -15.27
HI0362 -6.01 -7.29 -1.17 -2.96 -11.75 -31.98
HI0365 2.18 6.68 1.91 4.19 1.38 3.77
HI0502 ns -1.19 ns -1.67 -1.60 -11.24
HI0507 ns 16.41 2.27 10.47 2.04 5.76
HI0534 ns 1.31 -3.38 -9.02 -3.12 -5.49
HI0563 ns -1.1 -1.51 -1.03 ns -1.23
HI0601 -1.87 1.69 ns -1.16 -1.66 1.22
HI0661 -6.52 -2.36 -4.14 -7.08 -1.20 -1.59
HI0663m ns 1.08 -2.11 -1.98 -1.61 -1.87
HI0670 ns 1.27 -1.74 -1.50 -1.60 -1.74
HI0682 -5.03 -7.47 -4.18 -16.92 1.54 -8.05
HI0752 ns 1.23 -1.56 -1.49 ns 1.01
HI0774 ns 1.22 -2.31 -1.30 ns -1.26
HI0811 -2.20 -1.07 ns 1.06 ns 1.45
HI0833 ns -1.25 -1.64 -1.25 ns 1.64
HI0845 ns 1.20 -1.51 -1.65 ns 1.03
HI0889 1.83 5.82 1.68 4.90 -1.09 2.99
HI0890m 2.50 4.60 3.48 7.49 ns 3.20
HI1010 ns 5.52 1.91 12.00 1.86 1.32
HI1047 ns -1.4 -4.99 -2.58 -1.10 1.86
HI1064m ns 1.09 -1.57 -1.31 not on -1.45
HI1107 ns -5.42 -2.76 -3.42 -1.08 -1.66
HI1173 ns 2.24 2.93 1.62 1.52 1.88
HI1180 -5.68 -1.44 -2.47 -1.24 -1.20 1.42
HI1190 ns -1.01 -1.60 -1.72 -1.92 -1.73
HI1217 -2.75 1.1 -1.48 -2.20 -2.99 -3.60
HI1218 4.67 33.45 3.58 17.21 ns 11.58
HI1224 -1.66 -1.2 -1.68 -1.93 ns 1.13
HI1275 -2.01 1.07 -1.37 1.01 -3.34 -5.77
HI1305 ns 2.29 1.68 2.29 ns 1.71
HI1356 ns -1.83 -2.25 -1.83 -1.82 -2.15
HI1366 -1.66 -1.08 -1.59 -1.60 ns -1.42
HI1368 ns 2.24 ns 1.29 -1.86 -2.42
HI1383m ns -1.15 -1.78 -2.63 ns -1.72
HI1444 5.19 13.43 3.47 11.77 ns 7.21
HI1455 ns -2.24 -2.42 -1.23 ns 1.16
HI1546 ns 1.57 -1.74 1.10 ns 1.38
HI1607 2.30 2.08 ns 1.28 1.09 2.17
HI1612 2.27 2.41 1.50 -1.24 1.23 4.77
HI1693 ns -1.1 -1.52 -1.47 ns -1.11
HI1738 2.09 5.82 2.52 3.28 ns 2.38
tnaA e -16.22 -3.81 -4.93 -5.85 n/a n/a
  1. a The gene locus in Rd KW20
  2. b Fold change between the FeHm unsupplemented and supplemented sample from the microarray
  3. c Fold change between the FeHm unsupplemented and supplemented sample by Q-PCR of an independent experiment.
  4. d Fold change between the FeHm unsupplemented and supplemented sample by Q-PCR of the Rd KW20 previously published by Whitby et al. [25]
  5. e gene R2866v6.1850c- and Hib 10810.0830 in the R2866 and Hib 10810 genome respectively.
  6. not present on Rd array, but regulation verified by Q-PCR of the original Rd KW20 array samples.
  7. n/a – gene not present in this isolate
  8. Values in plain italic text show a disagreement in regulatory status between the microarray and Q-PCR data.