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Table 6 Real time qRT-PCR results for genes used to validate microarray data

From: Transcriptional adaptations following exercise in Thoroughbred horse skeletal muscle highlights molecular mechanisms that lead to muscle hypertrophy

Gene symbol

GenBank ID

Microarray

qRT-PCR

  

FC T1

P

FC T1

P

HSPA1A

CX602571

3.11

0.001

2.68

2.87E-04

PFKFB3

CX594334

1.84

0.023

2.30

0.010

FOS

CX604427

2.13

0.997

198.90

0.031

  

FC T 2

P

FC T 2

P

ATF4

CX603997

1.50

0.005

-2.04

0.014

BHLHE40

CX599005

1.44

0.008

2.25

0.014

CALM3

CX600504

-1.40

0.045

-1.22

0.031

CRTC2

CX604423

1.58

0.004

-1.12

0.483

Calm1

CX598227

1.49

0.004

2.36

0.010

HSPA1A

CX602571

4.84

1.6E-05

5.91

4.08E-04

PKM2

CX594899

1.63

0.028

1.05

0.648

  1. FC T1 represents the fold change in gene expression immediately post-exercise.
  2. FC T2 represents the fold change in gene expression four hours exercise post-exercise.