- Research article
- Open Access
Influence of genetic background on the occurrence of chromosomal rearrangements in Saccharomyces cerevisiae
© Fritsch et al; licensee BioMed Central Ltd. 2009
Received: 23 May 2008
Accepted: 06 March 2009
Published: 06 March 2009
Chromosomal rearrangements such as duplications and deletions are key factors in evolutionary processes because they promote genomic plasticity. Although the genetic variations in the Saccharomyces cerevisiae species have been well documented, there is little known to date about the impact of the genetic background on the appearance of rearrangements.
Using the same genetic screening, the type of rearrangements and the mutation rates observed in the S288c S. cerevisiae strain were compared to previous findings obtained in the FL100 background. Transposon-associated rearrangements, a major chromosomal rearrangement event selected in FL100, were not detected in S288c. The mechanisms involved in the occurrence of deletions and duplications in the S288c strain were also tackled, using strains deleted for genes implicated in homologous recombination (HR) or non-homologous end joining (NHEJ). Our results indicate that an Yku80p-independent NHEJ pathway is involved in the occurrence of these rearrangements in the S288c background.
The comparison of two different S. cerevisiae strains, FL100 and S288c, allowed us to conclude that intra-species genomic variations have an important impact on the occurrence of chromosomal rearrangement and that this variability can partly be explained by differences in Ty1 retrotransposon activity.
DNA double strand breaks occur spontaneously or as a result of DNA damaging agents such as ionizing radiations or chemical reagents. If this damage is not properly repaired, it can lead to the occurrence of chromosomal rearrangements such as duplications, deletions and translocations, which can affect cell growth and survival. These rearrangements are key events in genome reshaping and evolution processes and many of the genomes sequenced to date show traces of these rearrangements [1, 2]. In multicellular organisms, however, chromosomal rearrangements are often responsible for oncogenesis and for many human genetic diseases [3–5].
DNA double-strand break (DSB) repair mechanisms are therefore essential to each organism, since they preserve the integrity of the genome and prevent the deleterious effects of chromosomal rearrangements. These mechanisms can be classified in two distinct pathways: the homologous recombination (HR) pathway and the non-homologous end-joining (NHEJ) pathway. HR requires long homologous sequences for DSB repair whereas little or no homology is necessary for the NHEJ pathway.
In previous studies, rearrangements of several kinds were observed using this URA2-based screening method [6, 7]. In the haploid FL100 context, three types of rearrangements leading to ATCase reactivation were detected: Ty1 insertions downstream of the last point mutation in the ura215,30,72 allele, deletions of the region containing the three mutations, and duplications of the region encoding the ATCase followed by fusion with a new promoter sequence. Roelants et al. studied the ATCase reactivation resulting from Ty1 insertions and established that the transcription process is initiated in the LTR (Long Terminal Repeat) region of the Ty1 retrotransposon . Deletions of the mutated region in the ura215,30,72 allele were described by Welcker et al. and duplications of the ATCase region by Schacherer et al. [7, 9].
An analysis of variations at the nucleotide level in some commonly used Saccharomyces cerevisiae strains was carried out by Schacherer et al., who detected SNPs (Single Nucleotide Polymorphism) and deletions in various strains . These sequence differences may have important effects on several biological pathways and phenotypes. A total number of 22,446 SNPs and 53 deletions were identified when the FL100 strain was compared to the S288c strain and the divergence observed between the two strains amounted to 0.21% . To assess the effects of the genetic background on mutation rates and the type of chromosomal rearrangements, the ura215,30,72 genetic screening was used to select spontaneous rearrangements in the S288c context. The results were compared with those previously obtained in the FL100 background [6, 7].
Interestingly, in the S288c background, while duplications and deletions events were found to be responsible for the ATCase reactivation, no Ty1 insertions were observed. It was therefore concluded that the occurrence of chromosomal rearrangements is background-dependent. In addition, the occurrence of chromosomal deletions and duplications due to various recombination mechanisms was studied in haploid contexts. The impact of homologous recombination was tested by selecting revertants in a Δrad52 strain. Since Rad59p plays an important role in single-strand annealing (SSA) processes between direct repeats, a Rad59p deficient mutant, Δrad59, was constructed and selections of chromosomal rearrangements were performed in this background. The mutation rates and types of rearrangements observed in these two contexts were compared with a reference ura215,30,72 strain. Inhibiting RAD52-dependent homologous recombination increased the deletion rate, whereas inactivation of the SSA pathway increased the duplication rate. Secondly, the effects of non-homologous end joining (NHEJ) were tackled by mutating LIG4 and YKU80 in the ura215,30,72 background. The LIG4 mutation affected the mutation rates for both deletions and duplications, however there was no effect observed with YKU80 deletion. These results lead us to conclude that a Yku80p-independent NHEJ mechanism is responsible for the occurrence of chromosomal deletions and duplications.
The genetic background affects the occurrence of chromosomal rearrangements
Number of revertants obtained in the studied contexts and the corresponding mutation rates.
No. of revertants or ATCase reactivation events (mutation rate × 10-10) (95% confidence interval)
1.025 (0.676; 1.373)
0.707 (0.466; 0.947)
9 (31%)0.317 (0.210; 0.426)
1.69 (1.133; 2.247)
1.45 (0.974; 1.932)
0.237 (0.158; 0.314)
1.81 (1.27; 2.35)
0.977 (0.685; 1.269)
0.832 (0.584; 1.081)
0.997 (0.604; 1.39)
0.728 (0.441; 1.015)
0.269 (0.163; 0.375)
0.204 (0.055; 0.353)
0.204 (0.055; 0.353)
Among the 29 revertants leading to an ATCase reactivation, 20 (69%) carried deletions and 9 (31%) duplications. The boundaries of deletions were determined and microhomologies ranging from 1 to 11 bp in size were observed (see Additional file 1).
Contrary to what was observed in FL100 by Roelants et al., no reactivation by Ty1 insertions was found to occur in the S288c background although a greater number of deletions and duplications were observed in that background . These findings suggest that either there may be fewer Ty1 retrotransposons in S288c than in FL100 or that Ty1 retrotransposons may be less active in S288c than in FL100, and may thus be less likely to insert upstream from the ATCase region leading to a reactivation of the activity. Schacherer et al. showed that the proportion of Ty1 elements seems to be similar in these two backgrounds, hence the difference of Ty1 insertions may be explained by a variation of retrotransposons activity between FL100 and S288c .
The Ty1 activity is lower in the S288c than the FL100 background
Paquin and Williamson established that the temperature affects the rate of Ty transposition . They showed that the rate of transposition increases at temperatures below 30°C, which is the optimal growth temperature for Saccharomyces cerevisiae. In order to determine if the absence of Ty1 insertions in S288c resulted from a decrease in Ty1 transposition activity, selections were performed at 25°C. Twenty-four selections amounting to 1.04 × 1011 cells were performed at 25°C and we obtained 16 independent revertants. The mutation rate was determined to be: 3.70 × 10-10 mutations/cell/selection (CI: 2.12 × 10-10; 5.27 × 10-10). This mutation rate was 3.6 times higher than at 30°C. In addition, at 25°C, four Ty1 insertions (25%) were detected, whereas none were observed at the optimal growth temperature. These results led to the conclusion that the Ty1 retrotransposons are active in the S288c background but that they show lower rates of activity than those observed in the FL100 background.
The mechanisms leading to the occurrence of chromosomal deletions and duplications were studied using S288c at 30°C, in order to neglect Ty1 insertions and focus on the deletions and duplications events. Daley and Wilson showed that deletions can occur due to HR, SSA and NHEJ mechanisms, according to the DNA overhang length . Schacherer et al. proposed that the mechanism involved in the occurrence of genic duplications is a HR-dependent retroposition mechanism . Lastly Koszul et al. suggested that break induced replication (BIR) might lead to the appearance of segmental duplications . In this study we decided to focus on the impact of homologous recombination and non-homologous end joining processes in the occurrence of deletions and duplications.
Involvement of a homologous recombination mechanism in the occurrence of chromosomal deletions and duplications
The deletion rate increases in the Δ rad52 context
The first target used to investigate the impact of homologous recombination on the selection of chromosomal rearrangements was RAD52. Rad52p is a major component of HR: it binds to ssDNA as multimers and acts as a mediator allowing Rad51p to bind to ssDNA. It also plays a role in Rad51p-independent homologous recombination mechanisms. A deletion in RAD52 results in defective HR-associated double-strand break repair processes.
In this context, 83 selections corresponding to 2.4 × 1011 cells were performed leading to the occurrence of 21 independent revertants. The mutation rate in the aΔrad52 context was 1.69 × 10-10 mutations/cell/selection (CI: 1.13 × 10-10; 2.25 × 10-10), as shown in Table 1.
Among the 21 characterized revertants, 3 (14%) were duplications and 18 (86%) deletions (Table 1 and Figure 2B) with microhomologies ranging from 1 to 11 bp (see Additional file 1). There were no significant differences between the duplication rates, but the deletion rate was found to be twice as high as that recorded in the reference strain. This indicates that defective homologous recombination leads to an increased number of deletions.
The occurrence of duplications is repressed in RAD59
Using the approach described above, we used a strain mutated for a gene involved in homologous recombination and notably in the SSA pathway . Among the various candidates available, RAD59 was chosen. Rad59p is a protein which contributes to DNA DSB repair by annealing complementary single-strand DNA. In the aΔrad59 strain, 130 selections corresponding to 2.88 × 1011 cells were carried out and 37 revertants were isolated. The mutation rate in the aΔrad59 context was found to be 1.81 × 10-10 mutations/cell/selection (CI: 1.27 × 10-10; 2.35 × 10-10), and no statistical differences were observed in comparison with the reference strain.
The 37 revertants could be identified as 20 deletions (54%) and 17 duplications (46%). The microhomologies observed at the deletion boundaries ranged from 1 bp to 11 bp in size (see Additional file 1). The duplication rate was 2.5 times higher in this context than in the reference aUHT strain. This result is statistically significant whereas no significant difference was observed for the deletion rates. This allows us to conclude that Rad59p restrains the appearance of duplications.
Involvement of NHEJ in the occurrence of deletions and duplications
In order to test the impact of NHEJ on the occurrence of deletions and duplications, two deletion mutants, aΔyku80 and aΔlig4, were constructed in the ura215,30,72 background. Yku80p is a subunit of the Ku complex involved in telomere maintenance and in DNA binding at DSB sites, and Lig4p is the DNA ligase required for the NHEJ mechanism, along with the cofactors Lif1p and Nej1p.
The occurrence of deletions and duplications is Yku80p-independent
In the aΔyku80 context, 135 selections corresponding to 2.7 × 1011 cells were performed and 22 independent revertants were thus obtained. The mutation rate in the aΔyku80 context was 0.997 × 10-10 mutations/cell/selection (CI: 0.604 × 10-10; 1.39 × 10-10) (Figure 2B and Table 1). No significant differences were observed in this respect between aΔyku80 and the reference strain.
Among the 22 characterized revertants, 6 carried duplications (27%) and 16 deletions (73%) with microhomologies ranging from 2 to 11 bp in size (see Additional file 1). These results are in agreement with the data obtained for the reference strain. It was therefore concluded that in our system, deletions and duplications can occur in a Yku80p-independent context.
A Δlig4 context leads to a decrease of DNA repair
In the aΔlig4 background, 151 selections corresponding to 3.7 × 1011 cells were performed and 9 independent revertants were isolated. The mutation rate was found to be 0.204 × 10-10 mutations/cell/selection (CI: 0.055 × 10-10; 0.353 × 10-10), which corresponds to a statistically significant 5-fold decrease. Molecular characterization of the 9 revertants showed that all the reversion events resulted from deletions with microhomologies ranging from 1 to 11 bp in size (see Additional file 1). When LIG4 is inactivated, a decrease of efficient repair is observed. Moreover since the deletion rate was found to be 3.4 times lower in this context, it was concluded that Lig4p has an impact on the occurrence of deletions. Moreover, no duplications could be selected, which suggests that the Lig4p inactivation also inhibits the appearance of duplications in the ura215,30,72 system.
It was recently established that DNA sequence variations frequently occur among S. cerevisiae strains [10, 15]. These sequence differences may have important effects on the phenotypes [16–18]. However, little is known so far about the impact of genetic variations on the occurrence of chromosomal rearrangements.
Using the URA2-based genetic screening, we investigated the impact of the genetic background on the types of chromosomal rearrangements and the mutation rates.
Different genetic backgrounds lead to a variation of the type of selected chromosomal rearrangements
To test the impact of the genetic background, two S. cerevisiae strains, S288c and FL100 were compared in terms of rate of occurrence for various rearrangements such as deletions, duplications and Ty1 retrotransposon insertions. Interestingly, in the S288c context, no Ty1 retrotransposition insertions were selected, whereas these rearrangements account for 2/3 of the selected events in FL100. These findings point to the conclusion that either there may be fewer Ty1 retrotransposons in S288c than in FL100 or that they show lower levels of Ty1 activity.
Intra-species variations of retrotransposons location and number have been well described [19, 20]. Nevertheless, since the proportion of Ty1 elements seems to be similar in FL100 and S288c backgrounds, a difference of Ty1 activity can probably account for the differences between the Ty1 insertions .
Paquin and Williamson reported that Ty transposition is temperature-sensitive and that this process is enhanced at temperatures below the optimal growth temperature . The activity of the retrotransposons in the S288c background was therefore tested by selecting revertants at 25°C. At that temperature, 4 Ty1 insertions leading to ATCase reactivation were selected. This finding shows that Ty1 retrotransposons are active in the S288c background but their activity is probably decreased in that background compared to FL100.
In this study, intra-species variations were found to occur as regards the occurrence of chromosomal rearrangements between the S288c and the FL100 strains, and this variability was attributed to differences in the activity of the Ty1 elements. In addition, the regulation of the Ty1 retrotransposition might differ between the two strains. For instance, it has been established that S. cerevisiae undergoes transcriptional and post-transcriptional controls which limit transposition in a copy-number dependent manner [21, 22]. Differences in these control mechanisms might also explain the transposition variations observed between the two strains. Further studies on the FL100 strain are now required to determine how the activity of Ty1 retrotransposons is regulated and establish their exact locations.
Non-Homologous End Joining contributes to the occurrence of deletions
Contrary to what occurs in higher eukaryotes, where non-homologous recombination processes predominate, the main DNA double-strand break repair pathway in yeast is the homologous recombination pathway . It was therefore proposed to investigate the contribution of HR on the appearance of chromosomal deletions.
In a Δrad52 context, the deletion rate was found to be twice as high as in the reference strain, which suggests that the occurrence of deletions is favoured when RAD52-dependent homologous recombination is inactivated. This finding is in agreement with previously observed results in the FL100 background .
To form deletions by homologous recombination, a homologous sequence at least 60 bp long is required . However, since no sequence homologous to the ATCase-coding domain is present upstream from the URA2 gene, no ATCase reactivation is possible via the HR recombination pathway. In addition, since microhomologies (1 to 11 bp) were detected at the junctions of the deletions in every context, it was concluded that a mechanism of non-homologous recombination was probably responsible for the occurrence of the deletions.
When LIG4, the ligase required for NHEJ to occur, was mutated, we observed a decrease in deletion rate. However, when YKU80, which is also involved in NHEJ, was mutated, no differences were observed in comparison with the reference strain. Thus these results suggest that the repair mechanism involved in the occurrence of deletions is independent of Yku80p.
Boulton and Jackson established that two different NHEJ pathways can be distinguished in S. cerevisiae . One of these pathways is accurate and Ku-dependent, whereas the other one is error-prone, Ku-independent and involves short homologies. In addition, it has been suggested that the two NHEJ pathways might lead to different chromosomal rearrangements in S. cerevisiae . Given these observations, it seems likely that the occurrence of deletions in the ura215,30,72 context may be attributable to a Ku-independent NHEJ mechanism. These deletions would result from a DSB followed by a NHEJ repair involving short sequence homologies. In addition, when HR is inactivated, the rates of HR and NHEJ seem to be unbalanced and repair by NHEJ is favoured, thus leading to a higher rate of deletions.
We should mention that a compensatory mutation would have the same impact, since it would mask the effect of a YKU80 deletion. Nevertheless, this hypothesis is very unlikely since the Y16546 strain, from which the aΔyku80 background derives, was also used for functional analysis of a YKU80 deletion in other studies, showing one of the expected phenotype: the presence of short telomeres .
The occurrence of duplications depends on NHEJ
Rad59p is involved in homologous recombination and notably in the SSA mechanism, which involves short homologous sequences . In a Δrad59 context, a 2.5-fold increase in the duplication rate was observed, which suggests that inactivation of the SSA pathway may favour the occurrence of duplications.
On the other hand, in a Δlig4 context, the fact that no duplications were selected suggested that the duplication rate was greatly impaired. However, when YKU80 was mutated, no significant differences were observed in the duplication rates. It was therefore concluded that a Ku-independent NHEJ mechanism is involved in the occurrence of duplications in our system. Duplications may possibly result from two DSBs events followed by a NHEJ repair process occurring between two microhomologies at the DNA ends.
In this study, we investigated the mechanisms involved in the occurrence of chromosomal rearrangements and we tested the influence of intraspecies variability.
The results indicate that a non-homologous end joining mechanism independent of the Ku complex may be responsible for the occurrence of deletions and duplications in S288c. This repair mechanism is highlighted in absence of HR and SSA, which suggests that in a wild-type strain, a balance between the various mechanisms exists to maintain the integrity of the genome. The occurrence of deletions in the S288c context was similar to those previously suggested in a FL100 background . Although phenotypic differences and sequence variations can be observed from one strain to another, the use of major repair pathways such as HR and NHEJ remains unchanged.
By contrast, no Ty1 insertions were detected in S288c whereas these are major events contributing to ATCase reactivation in the FL100 background. This variation may be attributed to a difference in Ty1 activity rather than a difference between the mechanisms involved, since the repair mechanisms seems to be the same between FL100 and S288c when using the ura215,30,72 system. Further analysis of the activity of retrotransposons and their regulation in the FL100 background and a comparison of the activity in S288c may lead to confirmation of this hypothesis.
Yeast strains and media
Yeast strains used in this study.
MATα trp3 Δ63
Brachmann et al., 1998 
MATa his3 Δ200
Brachmann et al., 1998
BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YMR106c::KANMX4
Brachmann et al., 1998
BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YOR005c::KANMX4
Brachmann et al., 1998
BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YDL059c::KANMX4
Brachmann et al., 1998
BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YML032c::KANMX4
Brachmann et al., 1998
MATa ura215,30,72 his3 Δ200 trp1 Δ63
MATa ura215,30,72 his3 Δ200 trp1 Δ63 YMR106c::KANMX4
MATa ura215,30,72 his3 Δ200 lys2 Δ0 YOR005c::KANMX4
MATa ura215,30,72 his3 Δ200 lys2 Δ0 YML032c::KANMX4
MATa ura215,30,72 his3 Δ200 trp1 Δ63 YDL059c::KANMX4
Selection of Ura+ mutants
One isolated colony was grown in 300 μl YPD medium overnight, plated on YPD medium and incubated at 30°C for 4–5 days. The cells were then harvested, resuspended in 1.5 ml water and spread on supplemented YNB without uracil. The cells were then incubated at 30°C thus allowing the growth of spontaneous Ura+ revertants. In order to determine the total number of cells plated, a 100 μl aliquot of the culture was diluted 106 times, plated on YPD medium and incubated at 30°C. This procedure corresponds to one independent selection. The same procedure was used to perform selections at 25°C.
Mutation rate determination
The mutation rates (mutations/cell/selection) were determined using the maximum-likelihood method described by Lea and Coulson (1949) . The 95% confidence limits were calculated using Student's t-test.
Southern blot analysis
Genomic DNA from S. cerevisiae was prepared as described by Hoffman and Winston . DNA digestions were performed with the Bam HI restriction endonuclease (ROCHE) as described by the manufacturer. DNA digestions were migrated in a 1% agarose gel by electrophoresis and transferred onto a Hybond N+ membrane. Digoxygenin-labeled DNA probes were prepared using a DNA labeling kit (ROCHE) and detection was then carried out using a NBT/BCIP colorimetric method (ROCHE).
The type of chromosomal rearrangements present in the various selected revertants was determined by performing a Bam HI restriction pattern analysis based on Southern blot hybridization. A DNA probe specific to the ATCase domain was used and a single 6.8 kb band corresponding to the size of this domain was observed in the ura215,30,72 strain. A modified restriction profile was detected in all the selected revertants. In the case of a deletion or a Ty1 retrotransposon insertion, a single band differing in size from 6.8 kb was observed, whereas in the case of a duplication, two bands were detected: one corresponding to the ura215,30,72 allele and one corresponding to the duplicated ATCase coding region. We then discriminated between deletions and Ty1 insertions events using a PCR approach.
PCR amplification, DNA sequencing and sequence analysis
The primers used for PCR amplification and sequencing were chosen on the basis of the published genomic sequence of S288c. DNA fragments were obtained by performing PCR amplification using Taq DNA polymerase from MP Biomedicals, and the PCR conditions used were those described by the manufacturers. The Ty1 insertions in the URA2 coding sequence were characterized by performing PCR using two retrotransposon Ty1 LTR specific primers (sense and reverse) and a specific primer for the coding sequence of the ATCase. This allowed us to detect sense and reverse Ty1 insertions. The deletions were detected by performing primer-walking PCR to determine the presence or absence of a PCR product between close primer pairs. This made it possible to define the boundaries of the deletions.
The PCR products were purified using MicroSpin S400 (GE Healthcare). DNA sequencing was then performed on the purified fragments as described by Sanger et al. . The sequencing was carried out using AmpliTaq FS DNA polymerase and BIGDYE TM terminators. Sequence reactions were analyzed with an Applied Biosystems 373XL sequencer.
We would like to thank Malek Alioua in the Strasbourg CNRS/IBMP department for his help with the DNA sequencing and Jean-Luc Dortet-Bernardet for his help with the statistical analysis. We thank Nicolas Jauniaux for his helpful comments on the manuscript and Niamh Gilmartin for her help with copyediting the manuscript. This work was partly supported by the ANR-05-BLAN-0331-03 (GENARISE) and the Genolevure-3 sequencing consortium GDR CNRS 2354. ESF was supported by a grant from the French Ministère de l'Enseignement Supérieur et de la Recherche.
- Dunham MJ, Badrane H, Ferea T, Adams J, Brown PO, Rosenzweig F, Botstein D: Characteristic genome rearrangements in experimental evolution of Saccharomyces cerevisiae. Proc Natl Acad Sci USA. 2002, 99 (25): 16144-9. 10.1073/pnas.242624799.PubMed CentralView ArticlePubMedGoogle Scholar
- Wei W, McCusker JH, Hyman RW, Jones T, Ning Y, Cao Z, Gu Z, Bruno D, Miranda M, Nguyen M, Wilhelmy J, Komp C, Tamse R, Wang X, Jia P, Luedi P, Oefner PJ, David L, Dietrich FS, Li Y, Davis RW, Steinmetz LM: Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789. Proc Natl Acad Sci USA. 2007, 104 (31): 12825-30. 10.1073/pnas.0701291104.PubMed CentralView ArticlePubMedGoogle Scholar
- Difilippantonio MJ, Petersen S, Chen HT, Johnson R, Jasin M, Kanaar R, Ried T, Nussenzweig A: Evidence for replicative repair of DNA double-strand breaks leading to oncogenic translocation and gene amplification. J Exp Med. 2002, 196 (4): 469-80. 10.1084/jem.20020851.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharpless NE, Ferguson DO, O'Hagan RC, Castrillon DH, Lee C, Farazi PA, Alson S, Fleming J, Morton CC, Frank K, Chin L, Alt FW, DePinho RA: Impaired nonhomologous end-joining provokes soft tissue sarcomas harboring chromosomal translocations, amplifications and deletions. Mol Cell. 2001, 8 (6): 1187-96. 10.1016/S1097-2765(01)00425-7.View ArticlePubMedGoogle Scholar
- McKinnon PJ, Caldecott KW: DNA strand break repair and human genetic disease. Annu Rev Genomics Hum Genet. 2007, 8: 37-55. 10.1146/annurev.genom.7.080505.115648.View ArticlePubMedGoogle Scholar
- Roelants F, Potier S, Souciet JL, de Montigny J: Reactivation of the ATCase domain of the URA2 gene complex: a positive selection method for Ty insertions and chromosomal rearrangements in Saccharomyces cerevisiae. Mol Gen Genet. 1995, 246 (6): 767-73. 10.1007/BF00290725.View ArticlePubMedGoogle Scholar
- Welcker A, de Montigny J, Potier S, Souciet JL: Involvement of very short tandem repeats and the influence of the RAD52 gene on the occurrence of deletions in Saccharomyces cerevisiae. Genetics. 2000, 156: 549-557.PubMed CentralPubMedGoogle Scholar
- Roelants F, Potier S, Souciet JL, de Montigny J: Delta sequence of Ty1 transposon can initiate transcription of the distal part of the URA2 gene complex in Saccharomyces cerevisiae. FEMS Microbiol Lett. 1997, 148: 69-74. 10.1111/j.1574-6968.1997.tb10269.x.View ArticlePubMedGoogle Scholar
- Schacherer J, Tourrette Y, Souciet JL, Potier S, de Montigny J: Recovery of a function involving gene duplication by retroposition in Saccharomyces cerevisiae. Genome Res. 2004, 14 (7): 1291-7. 10.1101/gr.2363004.PubMed CentralView ArticlePubMedGoogle Scholar
- Schacherer J, Ruderfer DM, Gresham D, Dolinski K, Botstein D, Kruglyak L: Genome-wide analysis of nucleotide-level variation in commonly used Saccharomyces cerevisiae strains. PLoS ONE. 2007, 2 (3): e322-10.1371/journal.pone.0000322.PubMed CentralView ArticlePubMedGoogle Scholar
- Paquin CE, Williamson VM: Temperature Effects on the Rate of Ty Transposition. Science. 1984, 226 (4670): 53-55. 10.1126/science.226.4670.53.View ArticlePubMedGoogle Scholar
- Daley JM, Wilson TE: Rejoining of DNA double-strand breaks as a function of overhang length. Mol Cell Biol. 2005, 25 (3): 896-906. 10.1128/MCB.25.3.896-906.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Koszul R, Caburet S, Dujon B, Fischer G: Eucaryotic genome evolution through the spontaneous duplication of large chromosomal segments. EMBO J. 2004, 23 (1): 234-43. 10.1038/sj.emboj.7600024.PubMed CentralView ArticlePubMedGoogle Scholar
- Storici F, Snipe JR, Chan GK, Gordenin DA, Resnick MA: Conservative repair of a chromosomal double-strand break by single-strand DNA through two steps of annealing. Mol Cell Biol. 2006, 26 (20): 7645-57. 10.1128/MCB.00672-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Winzeler EA, Castillo-Davis CI, Oshiro G, Liang D, Richards DR, Zhou Y, Hartl DL: Genetic diversity in yeast assessed with whole-genome oligonucleotide arrays. Genetics. 2003, 163 (1): 79-89.PubMed CentralPubMedGoogle Scholar
- Steinmetz LM, Sinha H, Richards DR, Spiegelman JI, Oefner PJ, McCusker JH, Davis RW: Dissecting the architecture of a quantitative trait locus in yeast. Nature. 2002, 416 (6878): 326-30. 10.1038/416326a.View ArticlePubMedGoogle Scholar
- Yvert G, Brem RB, Whittle J, Akey JM, Foss E, Smith EN, Mackelprang R, Kruglyak L: Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors. Nat Genet. 35 (1): 57-64. 10.1038/ng1222.Google Scholar
- Deutschbauer AM, Davis RW: Quantitative trait loci mapped to single-nucleotide resolution in yeast. Nat Genet. 2005, 37 (12): 1333-40. 10.1038/ng1674.View ArticlePubMedGoogle Scholar
- Gabriel A, Dapprich J, Kunkel M, Gresham D, Pratt SC, Dunham MJ: Global mapping of transposon location. PLoS Genet. 2006, 2 (12): e212-10.1371/journal.pgen.0020212.PubMed CentralView ArticlePubMedGoogle Scholar
- Liti G, Peruffo A, James SA, Roberts IN, Louis EJ: Inferences of evolutionnary relationships from a population survey of LTR-retrotransposons and telomeric-associated sequences in the Saccharomyces sensu strict complex. Yeast. 2005, 22: 177-192. 10.1002/yea.1200.View ArticlePubMedGoogle Scholar
- Jiang YW: Transcriptional cosuppression of yeast Ty1 retrotransposons. Genes Dev. 2002, 16 (4): 467-78. 10.1101/gad.923502.PubMed CentralView ArticlePubMedGoogle Scholar
- Garfinkel DJ, Nyswaner K, Wang J, Cho JY: Post-transcriptional cosuppression of Ty1 retrotransposition. Genetics. 2003, 165: 83-99.PubMed CentralPubMedGoogle Scholar
- Aylon Y, Kupiec M: DSB repair: the yeast paradigm. DNA Repair (Amst). 2004, 3 (8–9): 797-815. 10.1016/j.dnarep.2004.04.013.View ArticleGoogle Scholar
- Sugawara N, Haber JE: Characterization of double-strand break-induced recombination: homology-requirements and single-stranded DNA formation. Mol Cell Biol. 1992, 12 (2): 563-575.PubMed CentralView ArticlePubMedGoogle Scholar
- Boulton SJ, Jackson SP: Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways. EMBO J. 1996, 15 (18): 5093-103.PubMed CentralPubMedGoogle Scholar
- Yu X, Gabriel A: Ku-dependent and Ku-independent end-joining pathways lead to chromosomal rearrangements during double-strand break repair in Saccharomyces cerevisiae. Genetics. 2003, 163 (3): 843-56.PubMed CentralPubMedGoogle Scholar
- Sugarawa N, Ira G, Haber JE: DNA length dependence of the single-strand annealing pathway and the role of Saccharomyces cerevisiae RAD59 in double-strand break repair. Mol Cell Biol. 2000, 20 (14): 5300-9. 10.1128/MCB.20.14.5300-5309.2000.View ArticleGoogle Scholar
- Lea DE, Coulson CA: The distribution of numbers of mutants in bacterial populations. J Genet. 1949, 49: 264-285. 10.1007/BF02986080.View ArticlePubMedGoogle Scholar
- Hoffman CS, Winston F: A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene. 1987, 57: 267-272. 10.1016/0378-1119(87)90131-4.View ArticlePubMedGoogle Scholar
- Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 1977, 74: 5463-5467. 10.1073/pnas.74.12.5463.PubMed CentralView ArticlePubMedGoogle Scholar
- Brachmann CB, Davies A, Cost GJ, Caputo E, Li J, Hieter P, Boeke JD: Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast. 1998, 14 (2): 115-32. 10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.