- Research article
- Open Access
The embryonic genes Dkk3, Hoxd8, Hoxd9 and Tbx1 identify muscle types in a diet-independent and fiber-type unrelated way
© de Wilde et al; licensee BioMed Central Ltd. 2010
Received: 5 November 2009
Accepted: 15 March 2010
Published: 15 March 2010
The mouse skeletal muscle is composed of four distinct fiber types that differ in contractile function, number of mitochondria and metabolism. Every muscle type has a specific composition and distribution of the four fiber types. To find novel genes involved in specifying muscle types, we used microarray analysis to compare the gastrocnemius with the quadriceps from mice fed a low fat diet (LFD) or high fat diet (HFD) for 8 weeks. Additional qPCR analysis were performed in the gastrocnemius, quadriceps and soleus muscle from mice fed an LFD or HFD for 20 weeks.
In mice fed the 8-week LFD 162 genes were differentially expressed in the gastrocnemius vs. the quadriceps. Genes with the strongest differences in expression were markers for oxidative fiber types (e.g. Tnni1) and genes which are known to be involved in embryogenesis (Dkk3, Hoxd8,Hoxd9 and Tbx1). Also Dkk2, Hoxa5, Hoxa10, Hoxc9, Hoxc10, Hoxc6 and Tbx15 were detectably, but not differentially expressed in adult muscle tissue. Expression of differentially expressed genes was not influenced by an 8-week or 20-week HFD. Comparing gastrocnemius, quadriceps and soleus, expression of Hoxd8 and Hoxd9 was not related with expression of markers for the four different fiber types. We found that the expression of both Hoxd8 and Hoxd9 was much higher in the gastrocnemius than in the quadriceps or soleus, whereas the expression of Dkk3 was high in quadriceps, but low in both gastrocnemius and soleus. Finally, expression of Tbx1 was high in quadriceps, intermediate in soleus and low in gastrocnemius.
We found that genes from the Dkk family, Hox family and Tbx family are detectably expressed in adult mouse muscle. Interestingly, expression of Dkk3, Hoxd8, Hoxd9 and Tbx1 was highly different between gastrocnemius, quadriceps and soleus. In fact, every muscle type showed a unique combination of expression of these four genes which was not influenced by diet. Altogether, we conclude that genes important for embryogenesis identify mouse muscle types in a diet-independent and fiber type-unrelated manner.
The mouse skeletal muscle is composed of four distinct fiber types, i.e., the type I, IIa, IIx and IIb fibers that differ in respect to contractile function, the number of mitochondria and metabolism. The slow type I fibers can sustain prolonged low power work, contain more mitochondria and exhibit higher rates of fat oxidative metabolism. The fast type IIx and IIb fibers are adapted to brief and intense contractions, contain fewer mitochondria and generate energy mainly through glycolysis. The type IIa fibers exhibit an intermediate contractile function and are oxidoglycolytic [1–4]. Every muscle type has a specific composition and distribution of the four different fiber types. The soleus contains the highest number of type I fibers, whereas both the quadriceps and gastrocnemius are muscle groups that contain predominantly type II fibers [5, 6]. However, varying percentages of type I fibers ranging from 0-45% for the quadriceps and 1-8% for the gastrocnemius are reported .
Numerous genes have been identified playing a role in the generation of more oxidative muscle types [6, 8–13]. The most extensively studied gene is calcineurin (CnA). In the C2C12 cell line, CnA up-regulates the mRNA levels of genes that are markers for the slow fiber types. Additionally, studies with CnA transgenic mice have reported an increase of oxidative fibers in the skeletal muscle [8, 9]. PGC1α is another well-established factor that induces remodeling of skeletal muscle fiber type composition. Lin et al.  showed that when PGC1α is expressed at physiological levels in muscle groups normally rich in type II fibers a fiber type conversion occurs from type II fibers to type I fibers . In line with this, Mortensen et al.  showed that PGC1α overexpression in primary rat skeletal muscle cells induces a switch towards a more oxidative fiber type. Finally, muscle-specific overexpression of PPARδ resulted in the increase of the number of oxidative fibers and in higher expression levels of several markers of oxidative metabolism [6, 12].
The first aim of the present study was to find novel genes that may play a role in specifying muscle types. Therefore we compared gene expression profiles of the gastrocnemius with the quadriceps at the level of the whole-transcriptome. Functional implications were assessed by the analyses of predefined gene sets based on Gene Ontology, biochemical, metabolic and signaling pathways. Recently, we studied the effects of a short-term high fat diet (HFD) on skeletal muscle gene and protein expression. Since both gene and protein levels of markers of the more oxidative fiber types were increased in the quadriceps of HFD mice we hypothesized that an HFD can induce a conversion towards a more oxidative fiber type via a transcriptional mechanism . The second aim of the present study therefore was to explore the diet-sensitivity of genes that are involved in the determination of muscle types. We compared expression patterns of genes that are differentially expressed between muscle types in mice fed a low fat diet (LFD) with mice fed an HFD.
Microarray analysis shows that the majority of genes involved in the determination of muscle types are not influenced by diet
Ten genes that showed the strongest increased or decreased expression in gastrocnemius compared to quadriceps
Strongest up-regulated genes
homeo box D9
homeo box D10
homeo box D8
troponin I, skeletal, slow 1
myosin, light polypeptide 2, regulatory, cardiac, slow
RIKEN cDNA 9830102E05 gene
aldehyde dehydrogenase family 1, subfamily A2
troponin T1, skeletal, slow
myosin, heavy polypeptide 7, cardiac muscle, beta
troponin C, cardiac/slow skeletal
Strongest down-regulated genes
dickkopf homolog 3 (Xenopus laevis)
dimethylarginine dimethylaminohydrolase 1
myosin binding protein H
phosphoenolpyruvate carboxykinase 1, cytosolic
cell death-inducing DFFA-like effector c
paternally expressed 3
cysteine dioxygenase 1, cytosolic
cDNA sequence BC054059
stearoyl-Coenzyme A desaturase 1
Overrepresented Gene Ontology classes in the gastrocnemius as compared to the quadriceps
blood vessel remodeling
vitamin A metabolic process
behavioral interaction between organisms
fat-soluble vitamin metabolic process
determination of bilateral symmetry
neural crest cell differentiation
regulation of muscle contraction
camera-type eye development
determination of left/right symmetry
mesenchymal cell differentiation
regulation of heart contraction
vitamin metabolic process
anterior/posterior pattern formation
Wnt receptor signaling pathway
hormone metabolic process
cellular metal ion homeostasis
skeletal muscle development
humoral immune response
striated muscle development
metal ion homeostasis
transmembrane receptor protein serine/threonine kinase signaling pathway
amino acid derivative metabolic process
respiratory tube development
integrin-mediated signaling pathway
calcium ion homeostasis
negative regulation of progression through cell cycle
nitrogen compound biosynthetic process
cellular cation homeostasis
cellular chemical homeostasis
protein amino acid dephosphorylation
positive regulation of immune response
positive regulation of multicellular organismal process
regulation of immune response
positive regulation of immune system process
regulation of immune system process
cell fate commitment
di-, tri-valent inorganic cation transport
regulation of cell size
cellular di-, tri-valent inorganic cation homeostasis
extracellular structure organization and biogenesis
negative regulation of signal transduction
regulation of growth
calcium ion transport
morphogenesis of an epithelium
immune effector process
regulation of kinase activity
regulation of protein kinase activity
regulation of transferase activity
Changed gene sets in the gastrocnemius versus quadriceps.
up-regulated cellular processes
Striated muscle contraction1
down-regulated cellular processes
Tissues, muscle, fat, bone and connective 21
CTCF first multivalent nuclear factor3
Valine, leucine and isoleucine degradation2
Cell cycle G1 to S control reactome2
Antigen processing and presentation2
Mets affect on macrophage differentiation3
Amino acid metabolism1
Porphyrin and Chlorophyll metabolism2
DNA replication reactome1
The diet-sensitivity of differentially expressed genes, overrepresented GO classes and regulated gene sets was studied by comparing the gastrocnemius with the quadriceps under HFD conditions (supplement 3). In the HFD mice we found 215 differentially expressed genes in the gastrocnemius as compared to the quadriceps. A total of 128 genes was overlapping with the differentially expressed genes in the LFD mice. Interestingly, these overlapping genes were all regulated in the same direction with a comparable fold change. A total of 87 genes was only differentially expressed in HFD mice, whereas 34 genes were differentially expressed in LFD mice suggesting that these genes are diet-dependent. Under HFD conditions, ErmineJ revealed that 193 GO classes were overrepresented in the gastrocnemius as compared to the quadriceps. A total of 67 GO classes showed an overlap with the overrepresented GO classes under LFD conditions. The other overrepresented GO classes corresponded also to contraction, morphogenesis, remodeling, development, calcium homeostasis, immune function and metabolic processes. GSEA showed that 11 gene sets were down-regulated including five gene sets describing cell cycle and four gene sets related to amino acid metabolism. Only one gene set "striated muscle contraction" was up-regulated in the gastrocnemius vs. the quadriceps. A total of six gene sets (five down-regulated and one up-regulated) were overlapping between the LFD and HFD condition.
To summarize, we showed that a considerable number of genes is differentially expressed in the gastrocnemius as compared with the quadriceps. The genes with the strongest regulations were markers for oxidative fiber types (Tnnc1, Tnni1, Tnnt1 and Myh7) and genes which are known to be involved in embryogenesis (Dkk3, Hoxd8, Hoxd9, Hoxd10 and Tbx1). The differentially expressed genes corresponded to a variety of process including morphogenesis and contraction, but also amino acid metabolism, cell cycle and immune function. Finally, we found more differentially expressed genes and more overrepresented GO classes under HFD conditions than under LFD conditions. However, this did not result in the identification of other regulated processes indicating that diet has only a very small effect on the difference between gastrocnemius and quadriceps.
Numerous genes involved in embryogenesis are detectably expressed in adult mouse muscle tissue
The Tbx1 gene is a muscle-type specific marker
Gene expression patterns of Hoxd8 and Hoxd9 do not correlate with expression patterns of markers for the different fiber types
In the present study we searched for novel genes that are involved in specifying muscle types. Additionally, we studied the diet-sensitivity of differentially expressed genes. By comparing the gastrocnemius with the quadriceps we identified 162 differentially expressed genes corresponding to a variety of biological processes. Especially processes involved in cell cycle, contraction, development, differentiation, morphogenesis and remodeling were differentially regulated in the gastrocnemius vs. the quadriceps. Both the gastrocnemius and the quadriceps are muscle groups that consist of predominantly type II fibers. In the present study we found that Myh7, Tnnc1, Tnni1 and Tnnt1, which are all markers for the more oxidative fiber types, are strongly up-regulated in the gastrocnemius as compared to the quadriceps. Western blotting showed that protein levels of myosin heavy chain, slow fiber type protein (marker for the oxidative type I fibers) were similar between the gastrocnemius and the quadriceps, whereas the protein level of Myh2 (marker for the oxidoglycolytic type IIA fibers) was higher in the gastrocnemius than in the quadriceps (data not shown). Therefore, we propose that in this study the gastrocnemius had a more oxidative character than the quadriceps.
Recently, we showed that a 4-week HFD intervention results in increased gene expression and protein levels of markers for the oxidative fiber types in the quadriceps of mice. These findings suggested that the skeletal muscle can adapt to an increased lipid load by inducing a switch to a more oxidative phenotype . Although the adult skeletal muscle is known to have the capacity to adapt to functional demands including exercise, hormones, innervation and mechanical loading [15, 16], a link between HFD and a switch of fiber type was not yet described. However, in the present study we showed that an 8-week HFD has none or only minor effects on expression levels of genes that play a role in specifying different muscle types including the markers for oxidative fiber types. Therefore we speculate that the up-regulation of markers for the oxidative fiber types is an early response of the skeletal muscle to the HFD which is not maintained in the long run.
Among the strongest regulated genes we found five genes (Dkk3, Hoxd8, Hoxd9, Hoxd10 and Tbx1) which are especially known for their critical role during embryogenesis. The skeletal muscle is a dynamic tissue containing different types of stem cells such as satellite cells, side-population (SP) cells and muscle-derived stem cells (MDSC) [17, 18]. To exclude the possibility that expression of these five embryogenesis-related genes originated from other cell types than myocytes we analyzed the expression of genes that specify satellite cells, SP cells and MDSC (satellite cells, pax7, myf5, cd34 and c-met; SP cells, sca1, sdc4 and pax3; MDSC, cd34 and Blc2 [17, 18]). We found that only three genes (cd34, sca1 and sdc4) were detectably expressed (microarray signal intensity > 20) in the skeletal muscle of these mice. However, none of these detectably expressed markers differed more than 1.3-fold between the gastrocnemius and the quadriceps. Altogether, we cannot exclude the presence of cells other than myocytes, however, it is most unlikely that these cells have contributed to the different expression levels of Dkk3, Hoxd8, Hoxd9, Hoxd10 and Tbx1.
Two genes, Dkk3 and Tbx1, were expressed at a lower level in the gastrocnemius than in the quadriceps. Extrapolating our data to the soleus we found that Dkk3 expression in the soleus was lower than in the quadriceps or gastrocnemius. Interestingly, Tbx1 expression levels in the soleus were lower than in the quadriceps, but higher than in the gastrocnemius. During embryogenesis Dkk3 and Tbx 1 are expressed in a variety of organs including the heart and limb buds [19, 20]. Whereas the function of Dkk3 is still poorly understood , Tbx1 is well-studied especially in relation to heart development . Recently it was shown that Tbx1 is also involved in development of the limb buds by regulating the number of myocytes . Since further research revealed that Tbx1 is not necessary for skeletal muscle specification, differentiation, patterning or activation of the embryonic myogenic program, this is the only described function of Tbx1 in relation to embryonic muscle development . Both Dkk3 and Tbx1 are reported to be detectably expressed in the adult skeletal muscle [19, 24], but until now nothing is known about their possible function. We speculate that the relatively high expression levels of Dkk3 and Tbx1 in the quadriceps is involved in maintaining identity of this muscle type.
Three genes of the Hoxd gene cluster (Hoxd8, Hoxd9 and Hoxd10) were up-regulated in the gastrocnemius as compared with the quadriceps. The Hox gene family consists of at least 39 Hox genes organized in four gene clusters (A, B, C and D) which are subdivided into parallel groups numbered 1 to 13. The major function of Hox genes is the regulation of the formation of anterior-posterior patterning during embryonic development . Different groups have studied the expression levels of the Hox gene family in adult tissues of human origin. They found that each tissue displays a unique combination of detectable Hox gene expression levels which is altered when normal cells changes to malignant cancer cells [26–28]. Thus, in addition to their critical role in embryonic development, Hox genes play an important role in adult cells by controlling critical processes like cellular identity and differentiation . Houghton et al. . have studied expression patterns of the Hox gene family in skeletal muscle tissue of adult mice. In line with our observations they reported that the Hoxa10, Hoxc6, Hoxc9 and Hoxc10 genes were detectably expressed. Furthermore, they could not detect Hoxd11 which also is consistent with our findings. Other members of the Hoxd gene cluster were not studied . Thus to our knowledge, we are the first to show that Hoxd8, Hoxd9 and Hoxd10 are detectably expressed in adult murine muscle tissue, with highly increased expression levels in the gastrocnemius as compared with the quadriceps.
Together with the higher Hoxd8 and Hoxd9 gene expression levels in the gastrocnemius we found increased gene expression levels of markers for the more oxidative fiber types. Therefore we hypothesized that Hoxd8 and Hoxd9 might be involved in the determination of more oxidative muscle types. However, when we extrapolated our data to the soleus muscle we could not find a corresponding expression pattern between Hoxd8, Hoxd9 and Myh7 (marker for type I fibers) and Tnni1 (marker for type I fibers; data not shown). In fact, Myh7 and Tnni1 were highly expressed in the soleus, whereas Hoxd8 and Hoxd9 expression in the soleus was as low as in the quadriceps. Also the expression patterns of Myh2, Myh1 and Myh4, which are markers for type IIA, IIX and IIB fibers, respectively, did not show any resemblance with the expression pattern of Hoxd8 and Hoxd9. Although we did not find any resemblances between expression patterns of Hoxd8, Hoxd9 and any of the fiber type-specific markers we could distinguish the gastrocnemius, quadriceps and soleus from each other. Altogether, we suggest that the high expression levels of the Hoxd8, Hoxd9 and possibly Hoxd10 genes are a specific characteristic of the molecular profile of the gastrocnemius and are not related to an oxidative phenotype.
We found that especially genes that are markers for oxidative fiber types and genes known to be important for embryogenesis were differentially expressed in the gastrocnemius vs. the quadriceps. The expression levels of these genes were not influenced by diet. Furthermore, expression of Hoxd8 and Hoxd9 was low and expression of Dkk3 and Tbx1 was high in the quadriceps. In the gastrocnemius we observed high expression of Hoxd8 and Hoxd9 and low expression of Dkk3 and Tbx1. Finally, in the soleus we found low expression of Hoxd8, Hoxd9 and Dkk3, but intermediate expression of Tbx1. Comparing gastrocnemius, quadriceps and soleus, the gene expression pattern of Hoxd8 and Hoxd9 did not correspond to the gene expression pattern of any of the fiber type-specific markers. Therefore, we conclude that the highly expressed embryonic genes Dkk3, Hoxd8, Hoxd9 and Tbx1 are involved in identifying muscle types in a diet-independent and fiber type-unrelated manner.
Mice and diet
Male C57BL/6J mice were obtained from Harlan (Horst, The Netherlands). At 9 weeks of age mice were switched to a run-in diet consisting of an LFD (10 kcal% fat) for 3 weeks. Following the run-in period mice were randomly assigned to the LFD or the HFD (45 kcal% fat). Both diets contained fat in the form of palm oil (based on D12450B and D12451; Research Diet Services, Wijk bij Duurstede, the Netherlands) as described previously .
After 8 weeks of diet intervention, mice (n = 10 per diet) were fasted for 6 hours, anesthetized with a mixture of isofluorane (1.5%), nitrous oxide (70%) and oxygen (30%) and killed by cervical dislocation. Gastrocnemius and quadriceps muscles were dissected, snap-frozen in liquid nitrogen and stored at -80°C until further analysis.
After 20 weeks of diet intervention, mice (n = 5 per diet) were fasted for 6 hour, anaesthetized and killed as described. Quadriceps, gastrocnemius and soleus muscles were dissected, snap-frozen in liquid nitrogen and stored at -80°C until further analysis.
Both animal studies were approved by the Local Committee for Care and Use of Laboratory Animals at Wageningen University.
Mouse total RNA was isolated from gastrocnemius, quadriceps and soleus muscles by mechanically homogenization in Trizol reagent (Invitrogen, Breda, the Netherlands). RNA was purified with the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) and contaminating genomic DNA was removed with the RNase-free DNase set (Qiagen). RNA quantity and purity was measured with the ND-1000 spectrophotometer (Isogen Life Science B.V., IJsselstein, the Netherlands). An A260/A280 ratio between 2.04 and 2.16 was found for all RNA samples. RNA integrity was checked on an Agilent 2100 BioAnalyzer (Agilent Technologies, Amsterdam, The Netherlands) using nanochips according to the manufacturer's instructions. All RNA samples showed an RIN value between 7.5 and 9.0.
Affymetrix microarray analysis
RNA isolated from the gastrocnemius and quadriceps of mice fed an 8-week LFD or HFD was used for performing Affymetrix GeneChip® Mouse Genome 430 2.0 arrays as described previously . Array images were processed using packages from the Bioconductor project  and probe sets were redefined according to Dai et al. . In this method probes are annotated using up-to-date databases and assigned to unique gene identifiers, in this case Entrez ID's, instead of the 'classic' GeneChip probe sets. This results in a less ambiguous and more accurate annotation. Arrays were normalized using quantile normalization, and expression estimates were calculated using GC-RMA, implementing the empirical Bayes estimate for non-specific binding . Differentially expressed probe sets were identified using linear models, applying moderated t-statistics that implement empirical Bayes regularization of standard errors . Comparisons were made between the gastrocnemius and quadriceps muscles using the fold change threshold method. In this method, most false-positives are caused by genes with low signal intensity and/or absent call (~50% of all genes). Thus, filtering out this constant noise before performing data analysis will improve the power to discriminate true changes from noise in the fold-change threshold method . We manually set the signal threshold at 20, across all arrays, filtering out 60% of the genes. Probe sets that satisfied the criteria of a fold change > 1.3 and an FDR < 0.05, correcting for multiple-testing , were considered as differentially expressed.
To relate changes in gene expression to functional changes two complementary methods were used. The first method is based on overrepresentation of Gene Ontology (GO) terms, which uses a gene score resampling (GSR) method . Full resampling was run with 200,000 iterations. Only classes with a false discovery rate (FDR) < 0.001 and with minimal 8 and maximal 125 genes were taken into account. The second method, gene set enrichment analysis (GSEA), is focused on predefined gene sets, that is, groups of genes that share biological function, chromosomal location or regulation . The 'functional catalogue' constructed by Subramanian et al. was modified to contain only 505 well-defined murine, biochemical, metabolic and signal pathways compiled from the following publicly available, curated databases: BioCarta (BioCarta. BioCarta, 2005, p. http://www.biocarta.com.), GenMAPP , Kyoto Encyclopedia of Genes and Genomes (KEGG) , Sigma-Aldrich pathways (Sigma-Aldrich. Sigma-Aldrich Metabolic and Cell Signaling pathways, 2005.) and Signal Transduction Knowledge Environment (STKE; Signal Transduction Knowledge Environment. In: http://stke.sciencemag.org/. 2005.). The analysis was run using 1000 permutations per gene set. Gene sets with a FDR < 0.05 were considered as significantly regulated. The advantage is that both methods are unbiased and a score is computed based on all genes in a GO term or gene set.
The diet-sensitivity of differentially expressed genes, overrepresented GO classes and regulated gene sets was studied by comparing the gastrocnemius with the quadriceps under HFD conditions. Also in this comparison we used the criteria of a fold change > 1.3 and an FDR < 0.05. A heatmap of log-transformed microarray signal intensities was generated by using GeneMaths XT software (Sint-Martens-Latem, Belgium). Array data have been submitted to the Gene Expression Omnibus, accession number GSE18127.
Quantitative real-time PCR for RNA
To validate microarray-detected changes as well as extrapolating microarray-detected changes to the gastrocnemius, quadriceps and soleus of 20-week LFD mice and HFD mice quantitative real-time PCR (qPCR) was performed using individual cDNA samples. RNA (1 μg in 20 μl) was reverse transcribed using the iScript cDNA synthesis kit (Biorad, Veenendaal, the Netherlands) containing RNase H+ iScript reverse transcriptase, a premixed RNase inhibitor to prevent indiscriminate degradation of RNA template, and a unique blend of oligo(dT) and random primers. cDNA was synthesized using a 3-step program (5 minutes (min) at 25°C, 30 min at 42°C and 5 min at 85°C). Primer sequences were retrieved from the online PrimerBank database , or otherwise designed using Beacon Designer5 (Biorad). Primers were tested for specificity by BLAST analysis. The qPCR reactions were performed in a volume of 25 μl containing 12.5 ng cDNA, 1× IQ SYBR Green Supermix (100 mM KCl, 40 mM Tris-HCI, 6 mM MgCl2, 0.4 mM of each dNTP, 50 units/ml iTaq DNA polymerase, SYBR Green I and 20 nM fluoresein; Biorad) and 400 nM of gene-specific forward and reverse primers (Biolegio, Nijmegen, the Netherlands). cDNA was amplified using a 2-step program (40 cycles of 10 seconds (sec) at 95°C and 45 sec at 60°C) with a MyiQ system (Biorad). Specificity of amplification was verified by melt curve analysis and evaluation of efficiency of PCR amplification. PCR reactions were performed in duplicate and gene expression levels were determined using iQ5 software (Biorad) using a Δ-Cq relative quantification model with PCR efficiency correction and multiple reference gene normalization [43, 44]. As the Canx, Hprt1 and Arbp genes were identified as most stably expressed reference genes, we used the geometric mean of these three reference genes as normalization factor. Supplement 4 shows all relevant primer information.
All data are expressed as means ± SE. Microarray data were analyzed as described above. All other statistical analyses were performed using Prism software (GraphPad Software, San Diego, CA, USA). Two-way ANOVA was used to test gene expression levels of Dkk3, Hoxd8, Hoxd9, Myh1, Myh2, Myh4, Myh7 and Tbx1 between muscle types and diets. When significant differences were found, a Bonferroni post hoc test was used to determine the exact location of the differences. A p value ≤ 0.05 was considered as statistically significant.
We thank Freek Bouwman, Mechteld Grootte Bromhaar, Shohreh Keshtkar Ghiasabadi and Bert Weijers for excellent technical assistance. This study was funded by the Top Institute Food and Nutrition, with financial support by the Dutch government.
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