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Table 2 Genes that are involved in major glutamate-related pathways and that are differentially expressed between the glu1-2 mutant and the wild-type mutant

From: Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome

EC #

Enzyme/protein

Abbreviation

Gene ID

log2 root

log2 leaf

1.4.1.7

Fd-dependent glutamate synthase

Fd-GOGAT 1/GLU1

AT5G04140

 

-5.738

1.4.1.7

Fd-dependent glutamate synthase

Fd-GOGAT 2/GLU2

AT2G41220

 

1.070

1.4.1.14

NADH-dependent glutamate synthase

NADH-GOGAT

AT5G53460

 

0.712

1.7.1.1

nitrate reductase

NIA2/NR2

AT1G37130

 

0.438

1.7.7.1

nitrite reductase

NIR1

AT2G15620

 

0.687

6.3.1.2

glutamine synthetase

GLN1.1

AT5G37600

 

1.296

6.3.1.2

glutamine synthetase

GLN1.3

AT3G17820

 

0.669

6.3.1.2

glutamine synthetase

GLN1.4

AT5G16570

 

-0.580

6.3.5.4

asparagine synthetase

ASN1

AT3G47340

 

-2.913

6.3.5.4

asparagine synthetase

ASN2

AT5G65010

 

-0.486

3.5.1.1

asparaginase

 

AT3G16150

 

-0.481

2.6.1.1

aspartate aminotransferase

ASP1

AT2G30970

 

0.582

2.6.1.1

aspartate aminotransferase

ASP2

AT5G19550

 

1.132

2.6.1.1

aspartate aminotransferase

ASP3

AT5G11520

 

0.709

2.6.1.1

aspartate aminotransferase

ASP4

AT1G62800

 

0.354

2.6.1.2

alanine:2-oxoglutarate aminotransferase

AlaAT1

AlaAT2

AT1G17290

AT1G72330

 

0.438

2.6.1.2/2.6.1.4

glutamate:glyoxylate aminotransferase

GGAT2

AT1G70580

 

-0.680

 

glycine decarboxylase complex -- H protein

AtGDH3

AT1G32470

0.593

 
 

glycine decarboxylase complex -- H protein

AtGDH1

AT2G35370

0.768

 

1.8.1.4

glycine decarboxylase complex -- L protein

AtmLPD1

AT3G17240

 

0.689

1.4.4.2

glycine decarboxylase complex -- P protein

AtGLDP1

AT4G33010

0.439

-1.126

1.4.4.2

glycine decarboxylase complex -- P protein

AtGLDP2

AT2G26080

 

-0.532

2.1.2.10

glycine decarboxylase complex -- T protein

AtGDT1

AT1G11860

 

-0.600

2.1.2.1

serine hydroxymethyltransferase

SHM1

AT4G37930

 

-0.513

2.1.2.1

serine hydroxymethyltransferase

SHM3

AT4G32520

 

0.532

2.1.2.1

serine hydroxymethyltransferase

SHM4

SHM5

AT4G13930

AT4G13890

 

0.535

4.1.1.15

glutamate decarboxylase

GAD3

GAD4

AT2G02000

AT2G02010

 

1.441

2.6.1.19

γ-aminobutyric acid transaminase

GABA-T1

AT3G22200

 

0.720

1.2.1.24

succinic semialdehyde dehydrogensase

SSADH1

AT1G79440

 

0.516

1.1.1.86

ketolacid reductoisomerase

KARI

At3g58610

 

0.380

4.2.1.9

dehydroxyacid dehydratase

DHAD

At3g23940

 

0.857

6.3.2.2

glutamate-cysteine ligase

GSH1

AT4G23100

 

0.587

6.3.2.3

glutathione synthetase

GSH2

AT5G27380

 

0.855

1.4.1.3

glutamate dehydrogenase

GDH2

AT5G07440

 

1.126

2.3.1.1

N-acetyltransferase

NAGS

AT4G37670

 

0.755

6.3.5.5

carbamoyl-phosphate synthase

CPS

AT3G27740

 

0.696

6.3.5.5

carbamoyl-phosphate synthase

CPS

AT1G29900

 

0.701

2.1.3.3

ornithine carbamoyltransferase

OTC

AT1G75330

 

0.436

6.3.4.5

arginosuccinate synthase

AS

AT4G24830

 

0.596

 

nitrate transporter

NRT1.1

AT1G12110

 

0.432

 

nitrate transporter

NRT1.3/NTP3

AT3G21670

 

-0.703

 

nitrate transporter

NRT1.5

AT1G32450

 

0.382

 

nitrate transporter

NRT1.7

AT1G69870

0.501

1.760

 

nitrate transporter

AtNRT2.7

AT5G14570

 

0.546

 

nitrate uptake

NRT3.1/NAR2.1

AT5G50200

 

0.939

 

nitrate/proton antiporter

AtCLCa

AT5G40890

 

-0.689

 

ammonium transporter

AMT2.1

AT2G38290

 

1.495

 

vacuolar tonoplast intrinsic protein

AtTIP2;1

AT3G16240

 

-0.554

 

vacuolar tonoplast intrinsic protein

AtTIP2;3

AT5G47450

-1.105

 
 

amino acid permease

AAP1

AT1G58360

 

-0.523

 

amino acid permease

AAP4

AT5G63850

-0.586

-0.470

 

amino acid permease

AAP5

AT1G44100

 

0.901

 

amino acid permease

AAP6

AT5G49630

 

-0.532

 

glutamate influx

LHT1

AT5G40780

-0.411

 
 

glutamate receptor family protein

AtGLR1.4

AT3G07520

 

-0.453

 

glutamate receptor family protein

AtGLR3.2

AT4G35290

 

-0.613

 

glutamate receptor family protein

AtGLR3.7

AT2G32400

 

0.338

  1. List of genes encoding enzymes involved in N-assimilation and glutamate biosynthesis and transformation described in the text, and whose expression is affected by knocking down Fd-GOGAT1 in the glu1-2 mutant. Most of the listed enzymes are also depicted in Figure 8. The presence of two gene IDs in some boxes indicates a nondiscriminating probe, i.e. a probe recognising more than one transcript so that a change in expression can not be attributed to a specific gene.