- Research article
- Open Access
Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae
© Kube et al; licensee BioMed Central Ltd. 2010
- Received: 1 September 2009
- Accepted: 22 June 2010
- Published: 22 June 2010
The genus Erwinia includes plant-associated pathogenic and non-pathogenic Enterobacteria. Important pathogens such as Erwinia amylovora, the causative agent of fire blight and E. pyrifoliae causing bacterial shoot blight of pear in Asia belong to this genus. The species E. tasmaniensis and E. billingiae are epiphytic bacteria and may represent antagonists for biocontrol of fire blight. The presence of genes that are putatively involved in virulence in E. amylovora and E. pyrifoliae is of special interest for these species in consequence.
Here we provide the complete genome sequences of the pathogenic E. pyrifoliae strain Ep1/96 with a size of 4.1 Mb and of the non-pathogenic species E. billingiae strain Eb661 with a size of 5.4 Mb, de novo determined by conventional Sanger sequencing and next generation sequencing techniques. Genome comparison reveals large inversions resulting from homologous recombination events. Furthermore, comparison of deduced proteins highlights a relation of E. billingiae strain Eb661 to E. tasmaniensis strain Et1/99 and a distance to E. pyrifoliae for the overall gene content as well as for the presence of encoded proteins representing virulence factors for the pathogenic species. Pathogenicity of E. pyrifoliae is supposed to have evolved by accumulation of potential virulence factors. E. pyrifoliae carries factors for type III secretion and cell invasion. Other genes described as virulence factors for E. amylovora are involved in the production of exopolysaccharides, the utilization of plant metabolites such as sorbitol and sucrose. Some virulence-associated genes of the pathogenic species are present in E. tasmaniensis but mostly absent in E. billingiae.
The data of the genome analyses correspond to the pathogenic lifestyle of E. pyrifoliae and underlines the epiphytic localization of E. tasmaniensis and E. billingiae as a saprophyte .
- Secretion System
- Fire Blight
- rRNA Operon
The genus Erwinia comprises essentially plant-associated bacteria. Two species, Erwinia amylovora and Erwinia pyrifoliae, are connected with "pome fruit" diseases, fire blight of apple, pear and some ornamentals and Asian pear blight, respectively [1–3]. Other species were isolated from plant surfaces such as Erwinia billingiae and Erwinia tasmaniensis. Due to their epiphytic occurrence, they can compete with growth and distribution of E. amylovora on flowers and may be applied as antagonists for control of fire blight . Such bacteria have the potential to reduce the use of antibiotics in agriculture for control of the disease. Accumulation of streptomycin resistant strains in pome fruit growing regions in the United States emphasizes the need of new strategies to reduce economical losses of more than $100 million per year for the United States resulting from fire blight [5–10].
In the course of description of pear pathogenic E. pyrifoliae strains, isolated in Korea, it became evident that they are related to E. amylovora, but distinct for several taxonomic criteria . A pathogen associated with bacterial shoot blight of pear (BSBP) in Japan was also classified as E. pyrifoliae . Strain Ep1/96 from Korea is assumed to be a representative for E. pyrifoliae and the features are also valid for the strains from Japan.
In dendrograms from 16 S rRNA sequences and in alignments of parts from the house keeping genes gpd and recA, E. pyrifoliae is related to E. amylovora, less to E. tasmaniensis and in more distance to E. billingiae .
Several properties of these species can explain their interactions with plants. Pathogens as well as epiphytic bacteria are dependent on the availability of carbohydrates to metabolize them as an energy source. Plants synthesize and transport high levels of sucrose as a main product of photosynthesis. E. amylovora, the fire blight pathogen, is specialized on rosaceous plants, which typically also produce sorbitol. Mutants of E. amylovora in the sucrose and in the sorbitol metabolism are non-pathogenic [13, 14]. Surprisingly, E. tasmaniensis lacks the srl-operon and is unable to metabolize sorbitol, although the epiphytes were isolated from the apple and pear flora [15, 16]. E. billingiae was isolated from a similar environment, initially designated as "white Erwinia herbicola" and later classified as a novel species [17, 18]. These bacteria are also detected in necrotic plant tissue from trees with fire blight (Geider et al., unpublished), but do not share important properties with E. amylovora . These include the inability of E. billingiae to cause a hypersensitive response (HR) on tobacco leaves, a lack of levan synthesis with a general deficiency to metabolize sucrose . A deficiency in levan formation was also observed for E. pyrifoliae . On the other hand, destruction of the host plant tissue, indicated by HR on non-host plants, is an important pathogenicity factor of E. amylovora and was also described for E. pyrifoliae [1, 19, 20].
Another important pathogenicity factor of E. amylovora and E. pyrifoliae is the formation of capsular exopolysaccharide (EPS). Gene clusters encoding functions for amylovoran synthesis of E. amylovora and pyrifolan synthesis of E. pyrifoliae have been described and mutants created in these genes by transposon and by site directed mutagenesis, which lost virulence [21, 22]. Other virulence factors of E. amylovora were described to include iron uptake, or defects in amino acid or nucleotide metabolism .
Therefore it was of special interest to show, if factors necessary for invasion can be identified in the genome of E. pyrifoliae and if their absence in the genome of E. billingiae and E. tasmaniensis can describe their epiphytic occurrence in plant surfaces.
We determined the complete genome of the pear-pathogen E. pyrifoliae strain Ep1/96, which is related to E. amylovora and of the non-pathogenic E. billingiae strain Eb661 [11, 24]. A mixed strategy of pyrosequencing and traditional Sanger sequencing was used to determine both new genome sequences. These data together with the previously published genome sequence of E. tasmaniensis provide databases for comparative analysis of virulence factors.
Genome composition and architecture of Erwinia billingiae strain Eb661, E. tasmaniensis strain Et1/99 and E. pyrifoliae strain Ep1/96
Chromosome features for the genomes of E. pyrifoliae strain Ep1/96, E. tasmaniensis strain Et1/99 and E. billingiae strain Eb661.
G+C content (%)
Protein coding (%)
Average size (bp)
G+C content (%)
assigned as pseudo
However, Figure 2 also illustrates that the larger chromosome size of E. billingiae strain Eb661 is mainly based on the presence of single genes scattered over the whole genome and not on the transfer of large gene clusters. The minor influence of phage integrations was estimated by the prediction of integrated regions (258 kb for strain Eb661, 177 kb for strain Et1/99 and 261 kb for strain Ep1/96) and of gene duplication corresponding to the number of potential paralogs (76 for strain Eb661, 57 for strain Et1/99 and 223 for strain Ep1/96). Cumulative GC skew analyses support the weak modulations of the chromosomes by these events by its regular run (Additional file 1).
General features of the determined extrachromosomal elements of E. pyrifoliae strain Ep1/96, E. tasmaniensis strain Et1/99  and E. billingiae strain Eb661.
Protein coding (%)
Average size (bp)
G+C content (%)
mobilization or transfer gene
Genetic elements with potential impact to virulence of pathogenic erwinias were summarized and the presence of the key genes is indicated for the analysed genomes (for details please see Additional file 3).
Secretion systems and effectors
hrp/hsv/dsp cluster (T3SS)
Hrp-associated enzymes (HAE)
Hrp/hrc secretion/translocation pathway
hrcUTSRQ, hrpPO, hrcN;hrpQIJLXYSAB; hrcJ, hrpDEFG, hrcC, hrpTV
Hrp elicitor/effector region (HEE)
hrpN, orfABC, hrpW, dspEF
Salmonella SPI-1-like T3SS
Surface presentation of antigens (SPA)
Cell invasion proteins
sicA, sipBCDA, sptP, prgHIJK, orgA
Invasion plasmid antigen (IPA)
T6S proteins and effectors
Effector and virulence associated
putative operon involved in virulence
Outer membrane protease
Maintenance of virulence plasmid
Virulence protein; regulator for acidic stress; integral membrane protein, outer membrane protein
msgA, phoPQ, mviN, ompX
Outer membrane invasion protein
Virulence associated protein
Capsular polysaccharide systems
Capsular polysaccharide- biosynthesis
amsGHIABCDEFKL, galFE/cpsGHIABCDEFKL, galFE
Putative cps biosynthesis system
rcsA, rcsB, rcsC, rcsF
rlsA, rlsB, rlsC
Sorbitol uptake, metabolization
Sucrose uptake, metabolization
Non-ribosomal peptide synth.
Adhesion & extracellular factors
foxR, dfoA, alcA
We focussed the analyses on the genetic environment encoded within the genomes with respect to secretion, production of exopolysaccarides, sorbitol and sucrose metabolism as well as on the presence of miscellaneous genes with potential impact to the virulence for the pathogenic erwinias.
The ability to secrete effector proteins and to colonize host plant tissue represents very important features. Differences in chromosome content were also identified by mapping the deduced proteins from the non-pathogenic strains on the chromosome of E. pyrifoliae strain Ep1/96 as well as by one by one comparison (Additional file 2, Figure S2A-E; Additional file 3). The data for several combinations are arranged in Additional file 2 and several secretion systems are classified in Additional file 3 for their presence or absence in the investigated genomes.
Common secretion systems of the Enterobacteriaceae such as the sec-independent (Type 1) and sec-dependent (Type 2) secretion systems have been identified in Erwinia species that fulfil vital functions, e.g. export of extracellular proteins for nutrient acquisition . Among them, one of the best studied are the genetically and structurally conserved type III secretion systems (T3SSs), which were found to be crucial for delivery of proteins acting as pathogenicity factors into the extracellular space or the cytoplasm [31, 32]. Target cells and secreted proteins are of broad range and host-specific.
The primary T3SS in Erwinia species is composed of the hrp/hrc-gene-cluster and two flanking regions (Hrp elicitors and effectors [HEE] and Hrp-associated enzymes [HAE]), which contain effector proteins and enzymes involved in systemic virulence [15, 33]. Like in E. amylovora this assembly could also be identified in the pathogenic E. pyrifoliae strain Ep1/96, and clearly marks a difference to the system of the non-pathogenic E. tasmaniensis strain Et1/99 without the HAE region and E. billingiae strain Eb661, which contains no homologs to a T3SS . Nevertheless, the hrp/hrc-clusters of E. pyrifoliae and E. tasmaniensis show almost conserved synteny. Slight differences are orfU1 (Acc. No. AAD24685) and orfU2 (Acc. No. AAD24686) of E. amylovora with similarity to genes coding for hypothetical proteins in Helicobacter pylori . The products of those CDS may represent specific components of the E. amylovora T3SS.
A second gene cluster similar to the HAE region could be identified in E. pyrifoliae. The two genes hsvA and hsvC show a high similarity on amino acid level of 86% and 66%, respectively, to their counterparts in the hrp/hrc-T3SS. However, a corresponding hsvB-gene was not found in this cluster but a sequence coding for a putative capsular exopolysaccharide synthesis protein.
An incomplete T3SS similar to the Salmonella pathogenicity island 1 (SPI-1) and also found in the insect endosymbiont Sodalis glossinidus, may have a distinct function in Erwinia species [15, 34]. This region, spanning about 20 kbp in E. pyrifoliae and E. tasmaniensis, contains most of the invasion- (invFGEABC), surface presentation of antigens (spaOPQRS) and invasins with the associated chaperone-genes (sicA, sipB, sipD) as well as those encoding the needle complex (prgHIJK, orgA). However, three CDS for conserved hypothetical proteins were found replacing sipC, an essential invasion gene and invIJ, encoding putative effectors [35, 36]. Furthermore, the genes sipA, iacP, sicB, sptP, iagB, hilA, orgBC and hilC, which constitute mainly regulatory components, are lacking. The gene content and order is highly conserved between E. pyrifoliae and E. tasmaniensis. A similar island was not found in E. billingiae. However, it remains unclear, if this T3SS is operative because of the incompleteness in comparison to the SPI-1 and the replaced genes. Recent results from pathogenicity tests on immature pears with SPI-1-like mutants of E. amylovora indicate that it is not essential for pathogenicity . Even for Salmonella typhimurium it was shown, that only the initial infection stages are affected in mutants while they remain pathogenic when applied by different routes .
Only few CDS for putative effector proteins could be identified in the erwinias. Most of those proteins are thought to affect or to be secreted by the T3SS. The suggested effector SrfC of Pectobacterium carotovorum subsp. atrosepticum is also thought to be exported by T3SS . E. billingiae carries the srfABC gene cluster like the other three erwinias, but is lacking the instrumentation for a T3SS. The function of SrfC remains unclear, in consequence.
Both pathogenic erwinias possess coding sequences for the SopA protein, which has been characterized as an effector-like protein in Salmonella influencing the inflammatory response of mammalian hosts . This protein is translocated via the Salmonella T3SS on the SPI-1 into eukaryotic cells and seems to be necessary for full virulence . Since a similar T3SS has been identified in the pathogenic erwinias, one could assume that the SopA effector has a particular role in pathogenicity of those bacteria in plants. It could influence proteins in the plant cell to alter defence response to bacterial invasion. Another putatively SPI-1 dependent system found in the four Erwinia species is composed of the small operon srfABC, which seems to be regulated by SPI-1 activation . Repression is accomplished by RcsB and PhoP, whose coding sequences could be identified in the erwinias. For several effectors the SPI-1 related T3SS may has a special function, which is different to the hrp/hrc-T3SS but probably not essential for virulence because it is also present in the non-pathogenic species E. tasmaniensis. This would be in accordance to rececently published results on SPI-1 mutants of E. amylovora .
The gene virK, which is secreted by the second Salmonella T3SS found on the pathogenicity island 2 (SPI-2) and regulated by the phoPQ-genes, is a pathogenicity factor of Salmonella sp. . A coding sequence for VirK was identified in E. tasmaniensis and E. billingiae but not in the pathogenic erwinias. A possible reason could be the missing secretion system for this protein, which led to the loss of the gene in the process of specialization. The T3SS share a wide homology that could support secretion by the other systems found in the Erwinia species . A simple protein export machinery is built by the Type V secretion system (T5SS), which is found in various bacteria . The main domains, a leader sequence and an extracellular effector domain, and an outer membrane export channel, are sometimes encoded on one sequence and constitute one protein. Because of the self-assembly and -export they were termed autotransporters. Another strategy, dubbed two-partner secretion, is characterized by separate expression of leader-effector protein and the leader-channel protein. Most effector proteins are involved in adherence, invasion and degradation . The non-pathogenic E. billingiae is the only species where we identified genes for corresponding autotransporters (EbC_25980, EbC_37340). They show similarities to the AidA domain family, which is mainly present in enteropathogenic bacteria, and pertactin, an autotransporter found in Bordetella sp., respectively. The primary role of the afore mentioned proteins is adherence to target structures. It may be possible that they substitute the function of missing fimbrial parts found in the other Erwinia species, a difference to strains Ep1/96 and Et1/99.
An emerging class of secretion systems, possibly related with pathogenicity, is the recently identified Type VI secretion system (T6SS) in Gram-negative bacteria . Analyses revealed one large (EpC_06160-EpC_06400, vgrG) and one small cluster (EpC_19520-EpC_19550) in E. pyrifoliae. Both clusters are present in E. tasmaniensis and E. billingiae, but show variations in gene content (Additional file 4). Most of the still uncharacterized genes are conserved within the clusters. Functions were assigned for the putative regulator Fha (involved in phosphorylation), the membrane associated proteins such as Lip (outer membrane lipoprotein), IcmF and DotU (inner membrane proteins), ClpV (ATPase) and Hcp and VgrG [46, 47]. The proteins Hcp and VgrG are secreted, Hcp building a tube-like structure for effector delivery, while VgrG may be an effector-activator or an effector itself . One CDS, found in E. pyrifoliae (EpC_06280) and E. billingiae (EbC_05860), of the larger cluster codes for a putative exported protein, which shows similarities of 50-65% to a protein of other plant-pathogenic bacteria such as Pectobacterium atrosepticum (syn. Erwinia carotovora ssp. atroseptica) and Pseudomonas syringae pv. tomato. In case this exported protein has an effector function, it would match the previous results, that E. tasmaniensis is missing many other effector proteins . Whether the secretion systems have an influence on pathogenicity is undiscernible so far, since only a rudimentary instrumentation was found in E. amylovora. Plant invasion, which was not confirmed for E. tasmaniensis, may be an important requirement for the function (i.e. translocation of proteins into dedicated host cells).
For the T6SS essential gene content, function assignment and structural determination is not well advanced. Most information exist for animal pathogens, but also plant pathogens may use T6SS . Nevertheless, the intrinsic role of the T6SS beside T3SS and/or T4SS has yet to be determined.
Genetics of EPS synthesis by E. pyrifoliae and E. billingiae
Several metabolic factors are considered to play an important role for causing disease in Erwinia-infected plants including synthesis of exopolysaccharides (EPS) i.e. amylovoran or related products and levan production as well as metabolism of sorbitol and sucrose .
The capsular EPS of E. amylovora is amylovoran, which apparently modulates recognition of the bacteria by plant defense mechanisms and is a main pathogenicity factor . The gene cluster for EPS-synthesis of pyrifolan by E. pyrifoliae also consists of 12 CDS (Additional file 3) with two adjacent genes for precursor synthesis. The encoded proteins are at least 85% similar and have a conserved order for E. pyrifoliae and E. amylovora. The repeating units of amylovoran and pyrifolan have the same sugar composition and identical linkages except a missing second side chain of glucose for pyrifolan .
No EPS has been identified so far for E. tasmaniensis, but there is a gene cluster for synthesis of capsular polysaccharide on the chromosome . E. billingiae possesses similar genes but produces an EPS. In alignments of Cps proteins from E. pyrifoliae and E. billingiae, CpsF (WceF) have a remarkable divergence, which may indicate specific functions for processing and assembly of the repeating units of capsular EPS for both species.
E. amylovora produces another EPS, levan, which serves as quickly generated shield against recognition by plant defence reactions . The secreted levansucrase, encoded by lsc, cleaves sucrose into glucose and fructose, which is subsequently polymerized to levan . Levan is not strictly necessary for virulence of E. amylovora . Also the non-pathogenic E. tasmaniensis possesses an lsc gene and produces levan . E. pyrifoliae lacks the lsc gene, but an orf (EpC_17920) coding for a protein similar to levanase was identified . Levanase belongs to the β-D-fructofuranosidases and can also cleave inulin and sucrose . Therefore, the enzyme could provide nutrients by cleavage of fructans in plant tissue, and may degrade levan from synthesizing bacteria, if it is secreted by E. pyrifoliae.
Sorbitol and sucrose metabolism
A dominant carbohydrate in rosaceous plants is the transport sugar alcohol sorbitol. In case of E. amylovora the proteins for its metabolization are encoded by the genes srlAEBDMR, which could also be identified for E. pyrifoliae and E. billingiae but not in the genome of E. tasmaniensis [13, 15]. Virulence assays of E. amylovora srl-mutants showed reduced symptoms on apple seedlings but only weak effects on pear slices due to their low content of sorbitol. The content of sorbitol is high in leaves and in transport tissue, and its amount varies during plant development .
An interesting feature of E. billingiae is its possible ability to metabolize xylitol. This sugar alcohol is widely distributed in nature and can interfere with bacterial activities . E. billingiae may degrade xylitol to avoid interference with its growth and to use it as a carbon source despite its low nutritional value. A similar gene is missing in the genomes of E. pyrifoliae strain Ep1/96 and the E. tasmaniensis strain Et1/99. On the other hand, E. tasmaniensis strain Et2/99 can grow with xylitol as carbon source .
Miscellaneous determinants with potential impact to virulence of pathogenic erwinias
A striking difference between the pathogenic species E. pyrifoliae and the non-pathogenic E. tasmaniensis is the existence of a nearly complete Type I fimbrial gene cluster in the latter one (Additional file 3). Enterobacterial Type I fimbriae are implied to be involved in cell attachment and adhesion to surfaces leading to biofilm formation . Beside being a virulence determinant the fimbriae may therefore be a tool of epiphytic bacteria for colonization and protection against environmental effects. Without production of a capsular polysaccharide by E. tasmaniensis, the fimbriae may replace the capsules to allow bacterial aggregation . This is supported by the lack of a Type I fimbrial gene cluster in E. billingiae, which produces EPS and may thus not depend on fimbriae. On the other hand, the existence of Type I fimbriae may induce plant defence responses comparable to the effect of FimH on mammalian cells .
While the distribution of Type I fimbriae genes seems to differ, all Erwinia species except E. billingiae possess a comparable incomplete instrumentation of K88 (F4) fimbriae genes. These fimbriae were found in enterotoxigenic E. coli strains and identified as virulence factors, to determine attachment to specific receptors of intestinal cells . The cluster, build up with faeBCDEFGHIJ, could be reconstructed except for the regulator FaeB and the component FaeJ, without known expression and function . However, a CDS coding for a possible regulatory protein was found in E. pyrifoliae instead of FaeB, leading to the assumption that functional F4 fimbriae participate in adhesion of these species. There are no hints if they are related to pathogenicity.
Another gene cluster first described in enterotoxigenic E. coli strains and also found in the erwinias forms the class 5 fimbriae with the surface antigen CS14 in the csuABCDE-operon . They are involved for E. coli in attachment to specific intestinal epithelial cells. Whether F4 and class 5 fimbriae have a cumulative effect by adhering to receptors of the same cells or aim at different cells and have specific functions is difficult to determine on the molecular level. Since both, pathogenic and non-pathogenic, Erwinia species possess these genes, their role in pathogenicity is unclear.
Furthermore, genes for the global regulator of fimbrial expression, the heat-stable nucleoid-structuring protein (hns), are present in the erwinias . In E. amylovora, E. pyrifoliae, E. billingiae and E. tasmaniensis, copies of an hns gene are located on the chromosome and also on a plasmid. E. tasmaniensis carries two chromosomal hns genes. The global regulator also affects EPS synthesis and the protein binds to the lsc promoter region of E. amylovora .
Strain Eb661 encodes several matrix components necessary for biofilm formation. Genes for cellulose synthesis (bsc- and bcs-operon) were identified as well as a CDS for the surface protein BapA. This instrumentation is similar to biofilm producing Salmonella species . Several potential adhesion factors such as hemaglutinin-like proteins may support surface colonization .
The three Erwinia species share a common set of genes for proteases and siderophore production, which are important virulence factors in soft rot erwinias . Two proteases (PtrA, PtrB) and siderophores (encoded by foxR, dfoA, alcA) may help in colonization and acquisition of nutrients [15, 66]. Interestingly, the protease operon for the secreted enzyme PrtA, connected with virulence of E. amylovora, is not represented in the genomes of E. pyrifoliae, E. tasmaniensis or E. billingiae .
Furthermore, virulence factors such as the cytotoxic necrotizing factors Cnf1 and Cnf2 were identified. These proteins were connected with pathogenic E. coli strains to alter signal transduction resulting in modified cell morphology and cell cycles as well as decreased phagocytosis of the bacteria [68, 69]. Cnf1 was shown to inhibit apoptosis, which may help bacteria to survive after cell invasion and grow in an adapted environment . In the case of the Erwinia species which have been investigated, cnf1- and cnf2-genes could be identified in all species except E. billingiae, a possible role of Cnf may be to weaken defence response therefore helping the bacteria to spread. Cnf is thought to influence transcription factors via effects on signal transduction .
The presence of the eppT gene in strain Ep1/96 may be a result of an integration event as it is indicated by the phage integrase located upstream. Similar proteins could be identified in other plant pathogens such as P. syringae pv. syringae, which has been involved in synthesis of the phytotoxin syringomycin . This list can be extended to several plant pathogens such as Ralstonia solanacearum and Xanthomonas axonopodis pv. citri, but also to the non-pathogenic species Pseudomonas fluorescens. The NRPSs vary in size (e.g. 3351 aa for Et1/99 compared to 7028 aa for Ep1/96) . The NRPS of E. pyrifoliae and the one of P. atrosepticum show a similar length (7028 aa/7523 aa) and nearly 6000 aa could be aligned with an identity of at least 41%.
The comparative analysis of E. billingiae strain Eb661, E. tasmaniensis strain Et1/99 and E. pyrifoliae strain Ep1/96 highlights the different genome organization within this genus driven by recombination events. The genomes of these epiphytic and pathogenic bacteria show high accordance of single genes and gene clusters, which are potential virulence factors in pathogenic species. The difference in lifestyle may depend on protein secretion and invasion into plants. E. billingiae lacks any T3SS in contrast to E. tasmaniensis and E. pyrifoliae. Differences between both pathogenic E. amylovora and E. pyrifoliae and the non-pathogenic E. tasmaniensis include a lack of the HAE-region in the hrp/hrc-T3SS, of the SopA and PagC proteins and the presence of a VirK protein and putatively of Type I fimbriae. E. billingiae shows much larger variations, e.g. apparently more possibilities for biofilm formation and adhesion, no synthesis or utilization of levan, the absence of a non-ribosomal peptide synthetase similar to EppT and a T3SS with the HAE-region, the SopA protein and possesses a VirK protein. Therefore, those components probably represent factors to describe pathogenic and non-pathogenic Erwinia species. Factors such as synthesis of EPS and levan, utilization of sugar and sugar alcohols as well as expression of proteases and siderophores may be related to nutrient acquisition and to modulate plant defence.
The role of the NRPS in the pathogenic strain Ep1/96 remains unclear. A phytotoxin could be an advantage in weakening the plant during the colonization and can explain differences in the habitat of Erwinia species.
Virulence for E. pyrifoliae may depend on the factors summarized in this section. Accumulation in the genome of E. pyrifoliae could be interpreted as the result of an evolutionary adaptation process resulting in the specialization as a plant pathogen. The genetic distance between the pathogenic Erwinia species and E. tasmaniensis is small, and larger to E. billingiae, which has a tendency to invade necrotic tissue of plants. Several differences in genes and their expression apparently restrict E. tasmaniensis to exist as an epiphyte on plant surfaces. This enables the species to survive especially on flowers and supports its potential to compete with pathogens such as E. amylovora and possibly E. pyrifoliae.
E. pyrifoliae strain Ep1/96 (DSM 12162) and E. billingiae strain Eb661 (DSM 17872) were cultured as described previously [11, 18]. DNA was isolated with the Genomic DNA kit (Qiagen, Hildesheim, Germany) according to the manufacturer's instructions. The genomic sequences were determined by whole genome shotgun sequencing using Sanger based sequencing technology and pyrosequencing.
The genomes of E. pyrifoliae strain Ep1/96 and E. billingiae strain Eb661 were covered by short insert shotgun libraries with 1.5 and 2.5 kb inserts and fosmid libraries with 37 kb inserts (CopyControl™ Fosmid Library Production Kit, Epicentre, Madison, U.S.A.) . End-sequencing was performed on recombinant plasmids using BigDye 3.1 chemistry and 3730XL capillary sequencers (ABI, Darmstadt, Germany) resulting in a 9-fold sequencing coverage for strain Ep1/96 and a 11-fold sequencing coverage for strain Eb661. The high number of fosmid reads (24,252 and 7,096) resulted in one uncovered chromosomal region for strain Ep1/96 and a complete physical coverage by fosmid clones of the chromosome of strain Eb661. To reduce finishing experiments pyrosequencing was performed using the GS20 sequencer for strain Ep1/96 and the GS FLX platform for strain Eb661 (both 454 life science/Roche) resulting in an additional 25-fold (1,043,447 reads) and 8-fold (194,142 reads) sequencing coverage, respectively.
Data assembly was performed for strain Ep1/96 within two steps. GS20 data were initially assembled by Newbler (454 life science/Roche) and the resulting contigs were fragmented using PERL scripts resulting in overlapping sequence fragments, which were assigned as forward and reverse reads in fasta format and the corresponding fasta quality files. The thus obtained faked reads were assembled together with the Sanger derived processed reads using PhredPhrap http://www.phrap.org. GS FLX data and Sanger derived reads for strain Eb661 were assembled using the Celera assembler . Assembled data for both projects were imported into Consed, edited and verified. Finishing experiments were performed by primer-walking on bridging clones and PCR products to improve sequence quality and gap closure .
Genome annotation and comparison
Protein coding sequences were predicted by Glimmer3 and manually curated in Artemis [79, 80]. Artemis was also used to calculate cumulative GC-skews [(G-C)/(G+C)] of the chromosomes. The genome sequences were annotated with HTGA . Structural rRNAs and tRNAs were determined using RNAmmer and tRNAscan-SE [82, 83]. Integrated bacteriophages were predicted by PhageFinder . Furthermore, sequence analysis was improved using the RAST analysis platform . NRPSs content was subsequently analysed by Pfam http://pfam.sanger.ac.uk/ and specificity prediction (NRPSredictor, http://www-ab.informatik.uni-tuebingen.de/toolbox/) .
Genome comparisons were performed for E. billingiae strain Eb661 (this study), E. tasmaniensis strain Et1/99, E. pyrifoliae strain Ep1/96 (this study) and partial sequences of E. amylovora strain Ea273 . The complete genome sequence of E. amylovora strain Ea273 (ATCC 49946) was published recently: FN666575, FN666576, and FN666577. Conserved synteny was examined using ACT with the big_blast.pl script (5000 bases sequence size; BLASTN algorithm) .
Core and pan genome for the deduced proteins were analysed by BLASTCLUST http://www.ncbi.nlm.nih.gov/ and parsed through a script to obtain intersections. Numbers were calculated using an identity of 60%, an alignment length of 70% and an e-value of 1e-6 at least as cutoffs for these analyses. The utilized scripts were implemented in a new public web-service at http://www.reziclust.molgen.mpg.de, which allows recalculation of results as well as performing new analyses. Results were parsed in addition through scripts for visualization of the present or absent potential orthologs within each genome.
Potential paralogs were estimated using BLAST . Deduced protein sets of the chromosomes were compared against themselves and filtered with MSPcrunch using a minimal identity of 90% for this purpose .
Data and strain access
The annotated sequences of E. pyrifoliae strain Ep1/96 have been deposited in Genbank/EMBL/DDBJ under accession numbers FP236842 (chromosome), FP928999 (pEp2.6), FP236827 (pEP03), FP236828 (pEP05), FP236829 (pEP36), and of E. billingiae strain Eb661 under accession numbers FP236843 (chromosome), FP236826 (pEB102) and FP236830 (pEB170).
The strains used for genomic sequencing are deposited in various international strain collections: Ep1/96 as DSM 12162, CFBP 4171; Eb661 (type strain) as DSM 17872, CFBP 6830, NCPPB 661, LMG 2613 and the previously sequenced E. tasmaniensis strain Et1/99 (type strain) as DSM 17950, CFBP 7177, NCPPB 4357, LMG 25318.
We acknowledge Julia Cekanov, Janina Thiel, Bernd Timmermann and Ines Müller (Berlin) for technical assistance, and Steffen Scheer, Sven Klages and Alfred Beck (Berlin) for computational support.
- Rhim SL, Volksch B, Gardan L, Paulin JP, Langlotz C, Kim WS, Geider K: Erwinia pyrifoliae, an Erwinia species different from Erwinia amylovora, causes a necrotic disease of Asian pear trees. Plant Pathology. 1999, 48 (4): 514-520. 10.1046/j.1365-3059.1999.00376.x.View ArticleGoogle Scholar
- Shrestha R, Koo J, Park D, Hwang I, Hur J, Lim C: Erwinia pyrifoliae, a causal endemic pathogen of shoot blight of Asian pear tree in Korea. The Plant Pathology Journal. 2003, 19 (6): 294-300.View ArticleGoogle Scholar
- Vanneste J: Fire blight: The disease and its causative agent, Erwinia amylovora. 2000, Wallingford, Oxon, UK; New York, NY, USA: CABI Pub, 370-View ArticleGoogle Scholar
- Jakovljevic V, Jock S, Du Z, Geider K: Hypersensitive response and acylhomoserine lactone production of the fire blight antagonists Erwinia tasmaniensis and Erwinia billingiae. Microbial Biotechnology. 2008, 416-424. 10.1111/j.1751-7915.2008.00043.x.Google Scholar
- Loper JE, Henkels MD, Roberts RG, Grove GG, Willett MJ, Smith TJ: Evaluation of Streptomycin, Oxytetracycline, and Copper Resistance of Erwinia amylovora isolated from Pear Orchards in Washington-State. Plant Disease. 1991, 75 (3): 287-290. 10.1094/PD-75-0287.View ArticleGoogle Scholar
- McManus PS, Jones AL: Epidemiology and Genetic-Analysis of Streptomycin-Resistant Erwinia amylovora from Michigan and Evaluation of Oxytetracycline for Control. Phytopathology. 1994, 84 (6): 627-633. 10.1094/Phyto-84-627.View ArticleGoogle Scholar
- McManus PS, Stockwell VO, Sundin GW, Jones AL: Antibiotic use in plant agriculture. Annu Rev Phytopathol. 2002, 40: 443-465. 10.1146/annurev.phyto.40.120301.093927.PubMedView ArticleGoogle Scholar
- Moller WJ, Schroth MN, Thomson SV: The Scenario of Fire Blight and Streptomycin Resistance. Plant Disease. 1981, 65 (7): 563-568. 10.1094/PD-65-563.View ArticleGoogle Scholar
- Russo NL, Burr TJ, Breth DI, Aldwinckle HS: Isolation of streptomycin-resistant isolates of Erwinia amylovora in New York. Plant Disease. 2008, 92 (5): 714-718. 10.1094/PDIS-92-5-0714.View ArticleGoogle Scholar
- Norelli JL, Jones AL, Aldwinckle HS: Fire blight management in the twenty-first century - Using new technologies that enhance host resistance in apple. Plant Disease. 2003, 87 (7): 756-765. 10.1094/PDIS.2003.87.7.756.View ArticleGoogle Scholar
- Kim WS, Gardan L, Rhim SL, Geider K: Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). Int J Syst Bacteriol. 1999, 49 (2): 899-906. 10.1099/00207713-49-2-899.PubMedView ArticleGoogle Scholar
- Geider K, Auling G, Jakovljevic V, Volksch B: A polyphasic approach assigns the pathogenic Erwinia strains from diseased pear trees in Japan to Erwinia pyrifoliae. Lett Appl Microbiol. 2009, 48 (3): 324-330. 10.1111/j.1472-765X.2008.02535.x.PubMedView ArticleGoogle Scholar
- Aldridge P, Metzger M, Geider K: Genetics of sorbitol metabolism in Erwinia amylovora and its influence on bacterial virulence. Mol Gen Genet. 1997, 256 (6): 611-619. 10.1007/s004380050609.PubMedView ArticleGoogle Scholar
- Bogs J, Geider K: Molecular analysis of sucrose metabolism of Erwinia amylovora and influence on bacterial virulence. J Bacteriol. 2000, 182 (19): 5351-5358. 10.1128/JB.182.19.5351-5358.2000.PubMed CentralPubMedView ArticleGoogle Scholar
- Kube M, Migdoll AM, Müller I, Kuhl H, Beck A, Reinhardt R, Geider K: The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia. Environ Microbiol. 2008, 10 (9): 2211-2222. 10.1111/j.1462-2920.2008.01639.x.PubMedView ArticleGoogle Scholar
- Geider K, Auling G, Du Z, Jakovljevic V, Jock S, Volksch B: Erwinia tasmaniensis sp. nov., a non-phytopathogenic bacterium from apple and pear trees. Int J Syst Evol Microbiol. 2006, 56 (12): 2937-2943. 10.1099/ijs.0.64032-0.PubMedView ArticleGoogle Scholar
- Billing E, Baker LAE: Characteristics of Erwinia-Like Organisms Found in Plant Material. Journal of Applied Bacteriology. 1963, 26 (1): 58-View ArticleGoogle Scholar
- Mergaert J, Hauben L, Cnockaert MC, Swings J: Reclassification of non-pigmented Erwinia herbicola strains from trees as Erwinia billingiae sp. nov. Int J Syst Bacteriol. 1999, 49 (2): 377-383. 10.1099/00207713-49-2-377.PubMedView ArticleGoogle Scholar
- Steinberger E, Beer S: Creation and complementation of pathogenicity mutants of Erwinia amylovora. Molecular plant-microbe interactions. 1988, 1 (3): 135-144.View ArticleGoogle Scholar
- Shrestha R, Park DH, Cho JM, Cho S, Wilson C, Hwang I, Hur JH, Lim CK: Genetic organization of the hrp genes cluster in Erwinia pyrifoliae and characterization of HR active domains in HrpNEp protein by mutational analysis. Mol Cells. 2008, 25 (1): 30-42.PubMedGoogle Scholar
- Bugert P, Geider K: Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Mol Microbiol. 1995, 15 (5): 917-933. 10.1111/j.1365-2958.1995.tb02361.x.PubMedView ArticleGoogle Scholar
- Kim WS, Schollmeyer M, Nimtz M, Wray V, Geider K: Genetics of biosynthesis and structure of the capsular exopolysaccharide from the Asian pear pathogen Erwinia pyrifoliae. Microbiology. 2002, 148 (12): 4015-4024.PubMedView ArticleGoogle Scholar
- Dellagi A, Brisset MN, Paulin JP, Expert D: Dual role of desferrioxamine in Erwinia amylovora pathogenicity. Mol Plant Microbe Interact. 1998, 11 (8): 734-742. 10.1094/MPMI.19220.127.116.114.PubMedView ArticleGoogle Scholar
- Sauer S, Freiwald A, Maier T, Kube M, Reinhardt R, Kostrzewa M, Geider K: Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS One. 2008, 3 (7): e2843-10.1371/journal.pone.0002843.PubMed CentralPubMedView ArticleGoogle Scholar
- Liu SL, Sanderson KE: Homologous recombination between rrn operons rearranges the chromosome in host-specialized species of Salmonella. FEMS Microbiol Lett. 1998, 164 (2): 275-281. 10.1111/j.1574-6968.1998.tb13098.x.PubMedView ArticleGoogle Scholar
- Falkenstein H, Zeller W, Geider K: The 29 Kb Plasmid, Common in Strains of Erwinia amylovora, Modulates Development of Fireblight Symptoms. Journal of General Microbiology. 1989, 135: 2643-2650.Google Scholar
- Mohammadi M, Moltmann E, Zeller W, Geider K: Characterisation of naturally occurring Erwinia amylovora strains lacking the common plasmid pEA29 and their detection with real-time PCR. European Journal of Plant Pathology. 2009, 124 (2): 293-302. 10.1007/s10658-008-9417-8.View ArticleGoogle Scholar
- Lamark T, Rokenes TP, McDougall J, Strom AR: The complex bet promoters of Escherichia coli: regulation by oxygen (ArcA), choline (BetI), and osmotic stress. J Bacteriol. 1996, 178 (6): 1655-1662.PubMed CentralPubMedGoogle Scholar
- Hayes F: A family of stability determinants in pathogenic bacteria. J Bacteriol. 1998, 180 (23): 6415-6418.PubMed CentralPubMedGoogle Scholar
- Hueck CJ: Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev. 1998, 62 (2): 379-433.PubMed CentralPubMedGoogle Scholar
- Coburn B, Sekirov I, Finlay BB: Type III secretion systems and disease. Clin Microbiol Rev. 2007, 20 (4): 535-549. 10.1128/CMR.00013-07.PubMed CentralPubMedView ArticleGoogle Scholar
- McCann HC, Guttman DS: Evolution of the type III secretion system and its effectors in plant-microbe interactions. New Phytol. 2008, 177 (1): 33-47. 10.1111/j.1469-8137.2007.02293.x.PubMedView ArticleGoogle Scholar
- Oh CS, Kim JF, Beer SV: The Hrp pathogenicity island of Erwinia amylovora and identification of three novel genes required for systemic infection. Molecular Plant Pathology. 2005, 6 (2): 125-138. 10.1111/j.1364-3703.2005.00269.x.PubMedView ArticleGoogle Scholar
- Dale C, Jones T, Pontes M: Degenerative evolution and functional diversification of type-III secretion systems in the insect endosymbiont Sodalis glossinidius. Mol Biol Evol. 2005, 22 (3): 758-766. 10.1093/molbev/msi061.PubMedView ArticleGoogle Scholar
- Osiecki JC, Barker J, Picking WL, Serfis AB, Berring E, Shah S, Harrington A, Picking WD: IpaC from Shigella and SipC from Salmonella possess similar biochemical properties but are functionally distinct. Mol Microbiol. 2001, 42 (2): 469-481. 10.1046/j.1365-2958.2001.02654.x.PubMedView ArticleGoogle Scholar
- Collazo CM, Zierler MK, Galan JE: Functional analysis of the Salmonella typhimurium invasion genes invl and invJ and identification of a target of the protein secretion apparatus encoded in the inv locus. Mol Microbiol. 1995, 15 (1): 25-38. 10.1111/j.1365-2958.1995.tb02218.x.PubMedView ArticleGoogle Scholar
- Zhao Y, Sundin GW, Wang D: Construction and analysis of pathogenicity island deletion mutants of Erwinia amylovora. Can J Microbiol. 2009, 55 (4): 457-464. 10.1139/W08-147.PubMedView ArticleGoogle Scholar
- Penheiter KL, Mathur N, Giles D, Fahlen T, Jones BD: Non-invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches. Mol Microbiol. 1997, 24 (4): 697-709. 10.1046/j.1365-2958.1997.3741745.x.PubMedView ArticleGoogle Scholar
- Bell KS, Sebaihia M, Pritchard L, Holden MT, Hyman LJ, Holeva MC, Thomson NR, Bentley SD, Churcher LJ, Mungall K: Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors. Proc Natl Acad Sci USA. 2004, 101 (30): 11105-11110. 10.1073/pnas.0402424101.PubMed CentralPubMedView ArticleGoogle Scholar
- Diao J, Zhang Y, Huibregtse JM, Zhou D, Chen J: Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Nat Struct Mol Biol. 2008, 15 (1): 65-70. 10.1038/nsmb1346.PubMedView ArticleGoogle Scholar
- Wood MW, Jones MA, Watson PR, Siber AM, McCormick BA, Hedges S, Rosqvist R, Wallis TS, Galyov EE: The secreted effector protein of Salmonella dublin, SopA, is translocated into eukaryotic cells and influences the induction of enteritis. Cell Microbiol. 2000, 2 (4): 293-303. 10.1046/j.1462-5822.2000.00054.x.PubMedView ArticleGoogle Scholar
- Garcia-Calderon CB, Casadesus J, Ramos-Morales F: Rcs and PhoPQ regulatory overlap in the control of Salmonella enterica virulence. J Bacteriol. 2007, 189 (18): 6635-6644. 10.1128/JB.00640-07.PubMed CentralPubMedView ArticleGoogle Scholar
- Detweiler CS, Monack DM, Brodsky IE, Mathew H, Falkow S: virK, somA and rcsC are important for systemic Salmonella enterica serovar Typhimurium infection and cationic peptide resistance. Mol Microbiol. 2003, 48 (2): 385-400. 10.1046/j.1365-2958.2003.03455.x.PubMedView ArticleGoogle Scholar
- Henderson IR, Navarro-Garcia F, Desvaux M, Fernandez RC, Ala'Aldeen D: Type V protein secretion pathway: the autotransporter story. Microbiol Mol Biol Rev. 2004, 68 (4): 692-744. 10.1128/MMBR.68.4.692-744.2004.PubMed CentralPubMedView ArticleGoogle Scholar
- Pukatzki S, McAuley SB, Miyata ST: The type VI secretion system: translocation of effectors and effector-domains. Curr Opin Microbiol. 2009, 12 (1): 11-17. 10.1016/j.mib.2008.11.010.PubMedView ArticleGoogle Scholar
- Pukatzki S, Ma AT, Sturtevant D, Krastins B, Sarracino D, Nelson WC, Heidelberg JF, Mekalanos JJ: Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci USA. 2006, 103 (5): 1528-1533. 10.1073/pnas.0510322103.PubMed CentralPubMedView ArticleGoogle Scholar
- Filloux A, Hachani A, Bleves S: The bacterial type VI secretion machine: yet another player for protein transport across membranes. Microbiology. 2008, 154 (6): 1570-1583. 10.1099/mic.0.2008/016840-0.PubMedView ArticleGoogle Scholar
- Shrivastava S, Mande SS: Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes. PLoS One. 2008, 3 (8): e2955-10.1371/journal.pone.0002955.PubMed CentralPubMedView ArticleGoogle Scholar
- Geider K: Structure, biosynthesis and regulation of capsular exopolysaccharide of Erwinia amylovora and other species and role in pathogenicity. Bacterial Polysaccharides, Current Innovations and future trends. 2009, 223-238.Google Scholar
- Bellemann P, Geider K: Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization. J Gen Microbiol. 1992, 138 (5): 931-940.PubMedView ArticleGoogle Scholar
- Geier G, Geider K: Characterization and Influence on Virulence of the Levansucrase Gene from the Fireblight Pathogen Erwinia amylovora. Physiological and Molecular Plant Pathology. 1993, 42 (6): 387-404. 10.1006/pmpp.1993.1029.View ArticleGoogle Scholar
- Geider K: Exopolysaccharides of Erwinia amylovora: structure, biosynthesis, regulation, role in pathogenicity of amylovoran and levan. Fire Blight: The Disease and its Causative Agent, Erwinia amylovora. 2000, Wallingford, Oxon/UK.- New York, NY, USA: CABI Pub., 117-140. full_text.View ArticleGoogle Scholar
- Wanker E, Huber A, Schwab H: Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli. Appl Environ Microbiol. 1995, 61 (5): 1953-1958.PubMed CentralPubMedGoogle Scholar
- Suleman P, Steiner P: Relationship between sorbitol and solute potential in apple shoots relative to fire blight symptom development after infection by Erwinia amylovora. Phytopathology (USA). 1994, 1244-1250. 10.1094/Phyto-84-1244. 84Google Scholar
- Darling AC, Mau B, Blattner FR, Perna NT: Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res. 2004, 14 (7): 1394-1403. 10.1101/gr.2289704.PubMed CentralPubMedView ArticleGoogle Scholar
- Zabner J, Seiler MP, Launspach JL, Karp PH, Kearney WR, Look DC, Smith JJ, Welsh MJ: The osmolyte xylitol reduces the salt concentration of airway surface liquid and may enhance bacterial killing. Proc Natl Acad Sci USA. 2000, 97 (21): 11614-11619. 10.1073/pnas.97.21.11614.PubMed CentralPubMedView ArticleGoogle Scholar
- Blumer C, Kleefeld A, Lehnen D, Heintz M, Dobrindt U, Nagy G, Michaelis K, Emody L, Polen T, Rachel R: Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology. 2005, 151 (10): 3287-3298. 10.1099/mic.0.28098-0.PubMedView ArticleGoogle Scholar
- Ashkar AA, Mossman KL, Coombes BK, Gyles CL, Mackenzie R: FimH adhesin of type 1 fimbriae is a potent inducer of innate antimicrobial responses which requires TLR4 and type 1 interferon signalling. PLoS Pathog. 2008, 4 (12): e1000233-10.1371/journal.ppat.1000233.PubMed CentralPubMedView ArticleGoogle Scholar
- Edwards RA, Puente JL: Fimbrial expression in enteric bacteria: a critical step in intestinal pathogenesis. Trends Microbiol. 1998, 6 (7): 282-287. 10.1016/S0966-842X(98)01288-8.PubMedView ArticleGoogle Scholar
- Bakker D, Willemsen PT, Willems RH, Huisman TT, Mooi FR, Oudega B, Stegehuis F, de Graaf FK: Identification of minor fimbrial subunits involved in biosynthesis of K88 fimbriae. J Bacteriol. 1992, 174 (20): 6350-6358.PubMed CentralPubMedGoogle Scholar
- Anantha RP, McVeigh AL, Lee LH, Agnew MK, Cassels FJ, Scott DA, Whittam TS, Savarino SJ: Evolutionary and functional relationships of colonization factor antigen i and other class 5 adhesive fimbriae of enterotoxigenic Escherichia coli. Infect Immun. 2004, 72 (12): 7190-7201. 10.1128/IAI.72.12.7190-7201.2004.PubMed CentralPubMedView ArticleGoogle Scholar
- Hildebrand M, Aldridge P, Geider K: Characterization of hns genes from Erwinia amylovora. Mol Genet Genomics. 2006, 275 (3): 310-319. 10.1007/s00438-005-0085-5.PubMedView ArticleGoogle Scholar
- Jonas K, Tomenius H, Kader A, Normark S, Romling U, Belova LM, Melefors O: Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy. BMC Microbiol. 2007, 7: 70-10.1186/1471-2180-7-70.PubMed CentralPubMedView ArticleGoogle Scholar
- Gottig N, Garavaglia BS, Garofalo CG, Orellano EG, Ottado J: A filamentous hemagglutinin-like protein of Xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker, is involved in bacterial virulence. PLoS One. 2009, 4 (2): e4358-10.1371/journal.pone.0004358.PubMed CentralPubMedView ArticleGoogle Scholar
- Toth IK, Pritchard L, Birch PR: Comparative genomics reveals what makes an enterobacterial plant pathogen. Annu Rev Phytopathol. 2006, 44: 305-336. 10.1146/annurev.phyto.44.070505.143444.PubMedView ArticleGoogle Scholar
- Oh CS, Beer SV: Molecular genetics of Erwinia amylovora involved in the development of fire blight. FEMS Microbiol Lett. 2005, 253 (2): 185-192. 10.1016/j.femsle.2005.09.051.PubMedView ArticleGoogle Scholar
- Zhang Y, Bak DD, Heid H, Geider K: Molecular characterization of a protease secreted by Erwinia amylovora. J Mol Biol. 1999, 289 (5): 1239-1251. 10.1006/jmbi.1999.2846.PubMedView ArticleGoogle Scholar
- Hofman P, Le Negrate G, Mograbi B, Hofman V, Brest P, Alliana-Schmid A, Flatau G, Boquet P, Rossi B: Escherichia coli cytotoxic necrotizing factor-1 (CNF-1) increases the adherence to epithelia and the oxidative burst of human polymorphonuclear leukocytes but decreases bacteria phagocytosis. J Leukoc Biol. 2000, 68 (4): 522-528.PubMedGoogle Scholar
- Rippere-Lampe KE, O'Brien AD, Conran R, Lockman HA: Mutation of the gene encoding cytotoxic necrotizing factor type 1 (cnf(1)) attenuates the virulence of uropathogenic Escherichia coli. Infect Immun. 2001, 69 (6): 3954-3964. 10.1128/IAI.69.6.3954-3964.2001.PubMed CentralPubMedView ArticleGoogle Scholar
- Fiorentini C, Gauthier M, Donelli G, Boquet P: Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation. Cell Death Differ. 1998, 5 (9): 720-728. 10.1038/sj.cdd.4400412.PubMedView ArticleGoogle Scholar
- Fiorentini C, Falzano L, Travaglione S, Fabbri A: Hijacking Rho GTPases by protein toxins and apoptosis: molecular strategies of pathogenic bacteria. Cell Death Differ. 2003, 10 (2): 147-152. 10.1038/sj.cdd.4401151.PubMedView ArticleGoogle Scholar
- Duchaud E, Rusniok C, Frangeul L, Buchrieser C, Givaudan A, Taourit S, Bocs S, Boursaux-Eude C, Chandler M, Charles JF: The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens. Nat Biotechnol. 2003, 21 (11): 1307-1313. 10.1038/nbt886.PubMedView ArticleGoogle Scholar
- Rausch C, Weber T, Kohlbacher O, Wohlleben W, Huson DH: Specificity prediction of adenylation domains in nonribosomal peptide synthetases (NRPS) using transductive support vector machines (TSVMs). Nucleic Acids Res. 2005, 33 (18): 5799-5808. 10.1093/nar/gki885.PubMed CentralPubMedView ArticleGoogle Scholar
- Bender CL, Alarcon-Chaidez F, Gross DC: Pseudomonas syringae phytotoxins: mode of action, regulation, and biosynthesis by peptide and polyketide synthetases. Microbiol Mol Biol Rev. 1999, 63 (2): 266-292.PubMed CentralPubMedGoogle Scholar
- Smith DJ, Earl AJ, Turner G: The multifunctional peptide synthetase performing the first step of penicillin biosynthesis in Penicillium chrysogenum is a 421,073 dalton protein similar to Bacillus brevis peptide antibiotic synthetases. EMBO J. 1990, 9 (9): 2743-2750.PubMed CentralPubMedGoogle Scholar
- Kube M, Beck A, Zinder SH, Kuhl H, Reinhardt R, Adrian L: Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1. Nat Biotechnol. 2005, 23 (10): 1269-1273. 10.1038/nbt1131.PubMedView ArticleGoogle Scholar
- Myers EW, Sutton GG, Delcher AL, Dew IM, Fasulo DP, Flanigan MJ, Kravitz SA, Mobarry CM, Reinert KH, Remington KA: A whole-genome assembly of Drosophila. Science. 2000, 287 (5461): 2196-2204. 10.1126/science.287.5461.2196.PubMedView ArticleGoogle Scholar
- Gordon D: Viewing and editing assembled sequences using Consed. Curr Protoc Bioinformatics. 2003, Chapter 11 (Unit 11 12):Google Scholar
- Delcher AL, Harmon D, Kasif S, White O, Salzberg SL: Improved microbial gene identification with GLIMMER. Nucleic Acids Res. 1999, 27 (23): 4636-4641. 10.1093/nar/27.23.4636.PubMed CentralPubMedView ArticleGoogle Scholar
- Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, Barrell B: Artemis: sequence visualization and annotation. Bioinformatics. 2000, 16 (10): 944-945. 10.1093/bioinformatics/16.10.944.PubMedView ArticleGoogle Scholar
- Rabus R, Kube M, Beck A, Widdel F, Reinhardt R: Genes involved in the anaerobic degradation of ethylbenzene in a denitrifying bacterium, strain EbN1. Arch Microbiol. 2002, 178 (6): 506-516. 10.1007/s00203-002-0487-2.PubMedView ArticleGoogle Scholar
- Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW: RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 2007, 35 (9): 3100-3108. 10.1093/nar/gkm160.PubMed CentralPubMedView ArticleGoogle Scholar
- Lowe TM, Eddy SR: tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997, 25 (5): 955-964. 10.1093/nar/25.5.955.PubMed CentralPubMedView ArticleGoogle Scholar
- Bose M, Barber RD: Prophage Finder: a prophage loci prediction tool for prokaryotic genome sequences. In Silico Biol. 2006, 6 (3): 223-227.PubMedGoogle Scholar
- Overbeek R, Begley T, Butler RM, Choudhuri JV, Chuang HY, Cohoon M, de Crecy-Lagard V, Diaz N, Disz T, Edwards R: The subsystems approach to genome annotation and its use in the project to annotate 1000 genomes. Nucleic Acids Res. 2005, 33 (17): 5691-5702. 10.1093/nar/gki866.PubMed CentralPubMedView ArticleGoogle Scholar
- Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG, Parkhill J: ACT: the Artemis Comparison Tool. Bioinformatics. 2005, 21 (16): 3422-3423. 10.1093/bioinformatics/bti553.PubMedView ArticleGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: (17):3389-3402. 10.1093/nar/25.17.3389.PubMed CentralPubMedView ArticleGoogle Scholar
- Sonnhammer EL, Durbin R: A workbench for large-scale sequence homology analysis. Comput Appl Biosci. 1994, 10 (3): 301-307.PubMedGoogle Scholar
- Maxson-Stein K, McGhee GC, Smith JJ, Jones AL, Sundin GW: Genetic Analysis of a Pathogenic Erwinia sp. Isolated from Pear in Japan. Phytopathology. 2003, 93 (11): 1393-1399. 10.1094/PHYTO.2003.93.11.1393.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.