- Research article
- Open Access
Proteome-wide survey of phosphorylation patterns affected by nuclear DNA polymorphisms in Arabidopsis thaliana
- Diego Mauricio Riaño-Pachón†1, 2,
- Sabrina Kleessen†1,
- Jost Neigenfind1,
- Pawel Durek1, 4,
- Elke Weber1, 5,
- Wolfgang R Engelsberger1,
- Dirk Walther1,
- Joachim Selbig2,
- Waltraud X Schulze1 and
- Birgit Kersten1, 3Email author
© Riaño-Pachón et al; licensee BioMed Central Ltd. 2010
- Received: 9 April 2010
- Accepted: 1 July 2010
- Published: 1 July 2010
Protein phosphorylation is an important post-translational modification influencing many aspects of dynamic cellular behavior. Site-specific phosphorylation of amino acid residues serine, threonine, and tyrosine can have profound effects on protein structure, activity, stability, and interaction with other biomolecules. Phosphorylation sites can be affected in diverse ways in members of any species, one such way is through single nucleotide polymorphisms (SNPs). The availability of large numbers of experimentally identified phosphorylation sites, and of natural variation datasets in Arabidopsis thaliana prompted us to analyze the effect of non-synonymous SNPs (nsSNPs) onto phosphorylation sites.
From the analyses of 7,178 experimentally identified phosphorylation sites we found that: (i) Proteins with multiple phosphorylation sites occur more often than expected by chance. (ii) Phosphorylation hotspots show a preference to be located outside conserved domains. (iii) nsSNPs affected experimental phosphorylation sites as much as the corresponding non-phosphorylated amino acid residues. (iv) Losses of experimental phosphorylation sites by nsSNPs were identified in 86 A. thaliana proteins, among them receptor proteins were overrepresented.
These results were confirmed by similar analyses of predicted phosphorylation sites in A. thaliana. In addition, predicted threonine phosphorylation sites showed a significant enrichment of nsSNPs towards asparagines and a significant depletion of the synonymous substitution. Proteins in which predicted phosphorylation sites were affected by nsSNPs (loss and gain), were determined to be mainly receptor proteins, stress response proteins and proteins involved in nucleotide and protein binding. Proteins involved in metabolism, catalytic activity and biosynthesis were less affected.
We analyzed more than 7,100 experimentally identified phosphorylation sites in almost 4,300 protein-coding loci in silico, thus constituting the largest phosphoproteomics dataset for A. thaliana available to date. Our findings suggest a relatively high variability in the presence or absence of phosphorylation sites between different natural accessions in receptor and other proteins involved in signal transduction. Elucidating the effect of phosphorylation sites affected by nsSNPs on adaptive responses represents an exciting research goal for the future.
- Gene Ontology
- Phosphorylation Site
- Phosphorylated Amino Acid
- Slim Term
- Single Nucleotide Polymorphism Mapping
Protein phosphorylation is one of the most important and best characterized post-translational modification (PTM) among the hundreds so far described [1–3]. Reversible site-specific phosphorylation of serine (S), threonine (T), or tyrosine (Y) by concerted actions of kinases and phosphatases plays a central role in virtually all cellular processes , especially in cell signaling of prokaryotes and eukaryotes, including plants [5, 6]. Phosphorylation of a protein can occur at multiple sites and can be catalyzed by different kinases, often in crosstalk with other types of PTM [1, 7, 8]. Phosphorylation/dephosphorylation of specific amino acids in proteins can have profound effects on protein structure, activity, stability, subcellular localization and interaction with other biomolecules , and it can create binding sites for specific modular domains . Interestingly, in the flowering plant Arabidopsis thaliana, the percentage of genes predicted to encode protein kinases (3% of the predicted proteome) is about twice as high as in mammals [10, 11]. Protein phosphorylation events have been found to be connected with the plant's response to diverse intrinsic and extrinsic factors, such as light, invasion of pathogens, hormones, temperature stress, and nutrient starvation [6, 12, 13].
Recent progress in mass spectrometry (MS)-based technologies and phosphopeptide enrichment methods have allowed to map in vivo phosphorylation sites for a wide variety of organisms in a high throughput manner [12–14]. This progress has prompted the creation of dedicated web-resources in the plant field, such as PhosPhAt  and P3DB . The availability of experimentally verified A. thaliana phosphorylation sites now enables in silico analyses of different phosphorylation site patterns on a proteome-wide scale. Recently, the conservation of protein phosphorylation sites within selected gene families could be shown in different plant species [7, 17]. In non-plant species, where the databases of phosphorylation sites are much more comprehensive than in A. thaliana, the in silico analysis of phosphoproteomic data has already produced interesting insights into evolutionary features of protein phosphorylation [18–21].
A loss of a single phosphorylation site by a non-synonymous (ns) single nucleotide polymorphism (SNP) that mutates the amino acids S, T, or Y at a phosphorylation site into any other amino acid can have profound effects on the molecular properties of the corresponding protein. In particular, the disruption of phosphorylation sites by such non-synonymous mutations can be associated with human diseases such as cancer. For example, the phosphorylation of T286 in wildtype cyclin D1 by the kinase GSK3B initiates its nuclear export and subsequent degradation in the cytoplasm . The authors suggested that the loss of this phosphorylation site by a somatic mutation is involved in causing nuclear accumulation of cyclin D1 in esophageal cancer and a generally increased oncogenic potential.
In A. thaliana, genomic DNA polymorphisms have been studied extensively during the last few years, initially using gene expression microarrays in order to identify single-feature polymorphisms (SFPs) . Especially SNPs, in several inbreed accessions can be applied to study the effects of these polymorphisms on other genome-wide features, such as phosphorylation sites.
Given the importance of protein phosphorylation, we were interested to study how phosphorylation sites and their patterns are affected by natural variation, namely nsSNPs in A. thaliana. In this study we analyzed the distribution of all phosphorylation sites taken from the recent version of PhosPhAt (version 3.0)  in the proteome of A. thaliana and we related their position to nsSNPs thus identifying losses of phosphorylation sites. For that purpose, we made use of A. thaliana SNPs identified recently by applying re-sequencing arrays [24, 25] and by re-sequencing with ultra-deep sequencing technologies such as Illumina/Solexa . Our aim was to analyze how the A. thaliana phosphorylation sites and their patterns are influenced by these nsSNPs.
Because the current dataset of experimental phosphorylation sites in A. thaliana is far away from covering the entire proteome, the results obtained from the experimental phosphorylation site dataset were contrasted with results produced by similar analyses of predicted phosphorylation sites in A. thaliana to attain more global hypotheses on protein phosphorylation patterns and the influence of nsSNPs on them.
Description of the phosphorylation site sets used in this study
Experimental phosphorylation sites from different phosphoproteomic studies in A. thaliana[27–39] were taken from PhosPhAt (version 3.0)  comprising a total of 7,178 unambiguously identified phosphopeptides mapping to 4,252 protein-coding loci. The proportional frequency of phosphorylated S, T, Y residues (pS, pT, pY) in this experimental set was similar to distributions reported previously, with 70.7% of pS, 20.7% pT, and 8.6% pY .
We were able to confirm that the majority of phosphorylation sites (71%) occur outside conserved protein domains, as noted previously . Specifically, pS and pT occurred within domains in only 22.4% and 36.8% of the cases. However, phosphorylated tyrosines were located inside protein domains in 49.8% of the cases. Similar behavior was observed for the set of predicted phosphosites (data not shown).
The set of predicted phosphorylation sites used in this study was also taken from PhosPhAt (version 3.0) . It comprised 75,296 high-confidence phosphopeptides (score ≥1; see Methods), identified in 21,711 protein-coding loci. The relative frequency of phosphorylated S, T, Y residues in this set of predicted phosphorylation sites was 37.0% pS, 42.5% pT and 20.5% pY.
Based on our sets of experimentally identified as well as high-confidence predicted phosphorylation sites (score ≥1), we looked for under- and overrepresented plant GO Slim terms among different sets of phosphoproteins: i) all proteins containing at least one pS; ii) all proteins containing at least one pT; iii) all proteins containing at least one pY; iv) all proteins containing at least one pS or at least one pT (p[ST]); v) all proteins containing at least one phosphorylated residue (p[STY]) (Additional file 1 for experimental phosphorylation sites; Additional file 2 for predicted phosphorylation sites). A comparison of the GO annotation of the p[STY]-protein sets based on experimental and predicted sites is shown in the Figure included in Additional file 3. In both, experimental and predicted datasets, the terms "catalytic activity", "kinase activity", "transferase activity", and "protein modification process" were overrepresented among proteins containing p[STY] sites. Also, several stress-related terms were found overrepresented in both. In experimental as well as predicted p[STY]-protein sets, proteins with function in "translation", "RNA binding", "biosynthetic process" were determined as underrepresented. However, the plant GO Slim terms "transcription factor activity" and "transcription regulator activity" were found overrepresented among the predicted p[STY]-protein set, while underrepresented in the experimental set.
For both, experimental and predicted phosphorylation sites, we then computed the agreement of GO Slim terms between protein sets representing different sites or combination of sites. We evaluated the significance (corrected p-value, FDR ≤5E-2) of the correlation between the following pairs of datasets: pS-pT, pS-pY, pS-p[ST], pS-p[STY], pT-pY, pT-p[ST], pT-p[STY], pY-p[ST], pY-p[STY]. For each dataset in the pair we registered whether a given GO Slim term was present or absent, and then compared the obtained binary series using the Pearson's correlation coefficient. None of the compared profiles were correlated in the experimental dataset, but in the dataset of predicted phosphoproteins we found a moderate correlation of overrepresented plant GO Slim term profiles between pS-p[ST] and pY-pT, with correlation coefficients of 0.39 and 0.38, respectively.
Distribution of phosphorylation sites across proteins
Proteins with 9 or more experimentally identified phosphorylation sites in A. thaliana. Proteins with phosphorylation hotspots appear in italics. a Proteins annotated with the GO term "nucleobase, nucleoside, nucleotide and nucleic acid metabolic process" (GO: 6139).
Number of phosphorylation sites
Number of p(S);p(T);p(Y)
ATRSP41 (Arabidopsis thaliana arginine/serine-rich splicing factor 41); RNA binding
Splicing factor PWI domain-containing protein
RSZ33 (Arginine/serine-rich Zinc knuckle-containing protein 33); nucleic acid binding/nucleotide binding/zinc ion binding
ATSC35 ("Arabidopsis thaliana arginine/serine-rich splicing factor 35, 35 kDa protein"); RNA binding
IQD14; calmodulin binding
SCL30 (SC35-like splicing factor 30); RNA binding
CINV1 (CYTOSOLIC INVERTASE 1); beta-fructofuranosidase
AFH1 (FORMIN HOMOLOGY 1); actin binding
Peptidyl-prolyl cis-trans isomerase cyclophilin-type family protein
RNA recognition motif (RRM)-containing protein
AHA1 (PLASMA MEMBRANE PROTON ATPASE); ATPase
RSZ32; nucleic acid binding
1-phosphatidylinositol-4-phosphate 5-kinase/zinc ion binding
ESP4 (ENHANCED SILENCING PHENOTYPE 4); binding
Similar to splicing factor PWI domain-containing protein [Arabidopsis thaliana] (TAIR:AT2G29210.1)
Peptidyl-prolyl cis-trans isomerase cyclophilin-type family protein
Kinesin motor protein-related
TOC159 (translocon outer membrane complex 159)
Transducin family protein/WD-40 repeat family protein
Zinc finger (DHHC type) family protein
RBOHD (RESPIRATORY BURST OXIDASE PROTEIN D)
VIP4 (VERNALIZATION INDEPENDENCE 4)
SCL30a (SC35-like splicing factor 30a); RNA binding
ATRSP31 (ARGININE/SERINE-RICH SPLICING FACTOR 31); RNA binding
IQD32 (IQ-domain 32); calmodulin binding
A. thaliana proteins with potential phosphorylation hotspots consisting of experimental phosphorylation sites in a window of 10 amino acids.
Number of significant windows
Number of phospho-sites in window of 10 amino acids (window start)
5(6), 6(4), 4(7), 5(1)
4(1538), 4(1545), 4(1557), 5(1543)
1-phosphatidylinositol-4-phosphate 5-kinase/zinc ion binding
4(442), 4(439), 5(441)
4(22), 5(28), 4(29)
Reticulon family protein (RTNLB5)
4(4), 5(3), 4(1)
CINV1 (CYTOSOLIC INVERTASE 1); beta-fructofuranosidase
ZIGA4 (ARF GAP-LIKE ZINC FINGER-CONTAINING PROTEIN ZIGA4); DNA binding
Transcriptional regulator family protein
SR33 (SC35-like splicing factor 33); RNA binding
Lectin protein kinase, putative
Leucine-rich repeat transmembrane protein kinase, putative
Camphor resistance CrcB family protein
Zinc finger (C3HC4-type RING finger) family protein
Kinesin light chain-related
RIK (RS2-INTERACTING KH PROTEIN)
CBS domain-containing protein
SCL30 (SC35-like splicing factor 30); RNA binding
F-box family protein
Mitochondrial transcription termination factor-related/mTERF-related
SRZ-22 (serine/arginine-rich 22)
Protein kinase family protein
NHL repeat-containing protein
ATRSP41 (Arabidopsis thaliana arginine/serine-rich splicing factor 41); RNA binding
ATSC35 ("Arabidopsis thaliana arginine/serine-rich splicing factor 35, 35 kDa protein"); RNA binding
Phosphorylation hotspots within proteins
For proteins with many experimentally identified phosphorylation sites, we analyzed the location of those sites within the protein, to identify potential hotspots of phosphorylation.
Based on the experimental sites, we determined several hotspot-containing proteins using window sizes of 5, 10, 15, and 20 amino acids (Additional file 5). We then applied the phosphorylation hotspot analysis to the predicted phosphorylation site dataset to get a more global view on potential phosphorylation hotspots in the A. thaliana proteome. For predicted sites as well, we were able to determine several phosphorylation hotspots in proteins for window sizes of 10, 20, 30 and 40 amino acid residues (Additional file 6 and 7).
In general, phosphorylation hotspots were preferentially located outside conserved protein domains (p-value: 6.88E-4 for experimental phosphoprotein sets, for window size 10; p-value < 1.0E-7 for predicted phosphoprotein sets for window sizes 20, 30, and 40).
To identify over- or underrepresented biological functions among the proteins containing at least one phosphorylation hotspot that consists of experimental phosphorylation sites, these proteins were tested against a reference set which contained all proteins with at least one experimental phosphorylation site. GO enrichment analyses revealed that the GO Slim term "nucleoplasm" (GO: 5654, from the cellular component ontology) was significantly overrepresented (p-value: 1.52E-3 for window size 10). This was confirmed by the proteins with hotspots consisting of predicted phosphorylation sites (p-value: 4.60E-4 for window size 20). Interestingly, among the proteins with predicted phosphorylation hotspots, "catalytic activity" (GO: 3824) was significantly underrepresented (p-value: 2.40E-3 for window size 40).
Effect of SNPs on proteins
The mapping of SNPs onto coding sequences allowed us to evaluate their effects on the coded amino acids, and thus the expressed proteins. There were 12,285,899 amino acid residues in the non-redundant set of the protein models represented in the reference genome. In total, 314,705 amino acids and 594 stop codons were affected by SNPs (2.56% of all amino acid residues), either in a synonymous (160,634; 1.31%) or non-synonymous (155,311; 1.26%) way.
Interestingly, S and T, two phosphorylatable amino acids, are more likely to be affected by nsSNPs than expected. By contrast, Y residues were 3 to 5 times less frequently affected by SNPs than S or T residues (data not shown).
Effects of SNPs on phosphorylation sites
In case of the predicted phosphorylation sites, we found for threonine a significant enrichment of non-synonymous substitutions in phosphorylated compared to non-phosphorylated threonine residues (data not shown). Analyzing the effect of each individual substitution in the predicted dataset, we observed several amino acid substitutions with a substitution cost of two that occurred in phosphorylation sites (S to D, S to K, T to E, T to Q, T to V, Y to E, Y to L and Y to T; Additional file 9 and 10). Regarding the enrichment and depletion of individual substitutions between phosphorylated and non-phosphorylated sites, we found that all substitutions were as likely to occur in predicted phosphorylation sites as in non-phosphorylation sites, except for the substitution T to N, which was more likely to occur in predicted phosphorylation sites than in non-phosphorylation sites, and the synonymous substitution T to T, which was more likely in non-phosphorylation sites. In that respect, it is important to note that asparagine (N) has been shown to mimic a phosphorylated serine, in a mutant of the A. thaliana K+ channel AKT2 (AT4G22200.1). Asparagine is an uncharged amino acid, but it is larger than both S and T. Mimicking the phosphorylation site could arise due to steric effects as suggested by Michard et al. . Thus, a substitution T to N might generate a constitutive phosphorylation site.
Losses of experimental phosphorylation sites
Among the experimental phosphorylation sites, we identified 86 sites (in 86 proteins) that were lost in at least one of the A. thaliana accessions by an exchange of a phosphorylation target amino acid S, T, or Y to any other amino acid (Additional file 11). For four proteins (AT1G01550.1, AT1G44800.1, AT1G62330.1, AT5G38310.1) the phosphorylated S was substituted to more than one other amino acid in the accessions analyzed. For example a pS (position 341) of the nodulin MtN21 family protein (AT1G44800.1) was exchanged to T in most of the accessions with the only exception of Bur-0, where an N was introduced by the nsSNP.
Interestingly, we found that among these 86 proteins, the GO Slim categories "receptor activity" was significantly overrepresented, but there were no underrepresented categories. Using a reference set comprising all A. thaliana proteins with at least one phosphorylation site, no over- or underrepresented categories could be identified.
Remarkably, some losses of experimentally verified phosphorylation sites are located in phosphorylation hotspots. This concerns the following A. thaliana proteins: two proteins of unknown function (AT2G35880.1; pS at position 118 and AT3G18180.1; pS at position 67); a calmodulin-binding protein-related (AT3G29310.1; pS at position 327), and the protein ESP4 (ENHANCED SILENCING PHENOTYPE 4); binding (AT5G01400.1; pS at position 1364), based on TAIR7 annotation.
Gains and losses of predicted phosphorylation sites
Using the dataset of predicted high-confidence phosphorylation sites for a more global analysis, we found 1,114 proteins in which predicted phosphorylation sites could potentially be lost in at least one of the A. thaliana accessions studied (Additional file 12). In 1,103 cases, the SNP caused an amino acid substitution to an amino acid that cannot be phosphorylated, while in the remaining 11 proteins, the score of the phosphorylation prediction changed from a high-confidence positive score (score ≥1) to a value smaller than or equal to -1, regardless of the type of putative phosphorylated amino acid at the given position in the neighborhood of the affected phosphorylation site. A prediction value of score ≤ -1 is taken as high-confidence prediction of the amino acid not being phosphorylated.
In contrast, we observed that 1,148 proteins gained a predicted phosphorylation site (Additional file 12). The majority of gained phosphorylation sites (1,136) emerged by the change of a non-phosphorylatable amino acid to an S, T, or Y residue and a resulting prediction score ≥1 of the newly generated putative phosphorylation site. In the remaining 12 cases, the gain of the phosphorylation site was based on the score alone, the prediction score increased from a negative score (score ≤ -1) to a score higher than or equal to 1 due to nsSNPs that resulted in amino acid exchanges in the neighborhood to S, T and Y residues thus creating a new phosphorylation site target motif.
We found that the GO categories "receptor activity", "binding" and "signal transducer activity" were significantly overrepresented in all three datasets regardless of the reference set used (see Methods; Additional file 13). Additionally, in proteins which gained a phosphorylation site, and in all proteins with a gain or a loss, the category "response to stress" was overrepresented, regardless of the reference set used (Additional file 13). In the dataset including gain and loss of phosphorylation sites, the categories "transporter activity", "catalytic activity", "metabolic process", "biosynthetic process" and "cell" were underrepresented using the set of predicted phosphoproteins as reference set (Additional file 12). The figure in Additional file 14 presents all over- and underrepresented GO Slim categories in the protein set with losses and gains of predicted phosphorylation sites.
With regard to the isolation of phosphorylated proteins from complex samples as well as their mass spectrometric and computational analysis the progress that has been made in A. thaliana is quite impressive. However, the connection of protein phosphorylation with genetic variation in natural occurring ecotypes at a proteome-wide level in plant species has not been addressed so far. In this study we made use of experimental and predicted phosphorylation sites available in PhosPhAt  and mapped the data onto the A. thaliana genome annotation. This is currently the largest phosphorylation site dataset in A. thaliana comprising 7,178 experimentally verified unique phosphorylation sites assigned to 4,252 protein-coding loci. The combined data set represents phosphorylation sites identified from different tissues/cell types representing varied developmental stages and responses.
Since the assigned proteins cover only one-sixth of the predicted A. thaliana protein-coding loci, we extended our study by also including predicted phosphorylation sites to achieve better proteome coverage in our analyses, attaining a coverage of 80% of all protein-coding loci in A. thaliana.
The relative frequency of 70.7% pS, 20.7% pT, and 8.6% pY in our experimental set was similar to distributions reported previously in A. thaliana (; 85.0% pS, 10.7% pT, and 4.3% pY) and in humans [41, 42]. In the predicted dataset, the fraction of tyrosine residues was much higher (25.0%) compared to the experimental set (8.6%). This discrepancy may result from a bias in the training set of experimental phosphopeptides that has been used for the phosphorylation site predictor or from a lack of accuracy of the predictor. In addition, we cannot exclude that the experimentally identified phosphorylation sites may in general be biased by experimental restrictions such as specific phosphopeptide enrichment methods  or by a focus on specific stress conditions or subcellular compartments. Thus, this distribution may still shift by including new phosphorylation sites from future studies. However, since in general experimental and predicted data show similar distribution among and within the proteins, the inclusion of predicted data in the scope of this study is justified.
Multisite phosphorylation in proteins has been discussed as points of integration of different signal transduction pathways [1, 44]. However, a major difficulty in studying multisite phosphorylation from a system's perspective has been the uncertainty whether simultaneous or successive phosphorylation occurred at the different sites on the same protein and whether the multiple sites were phosphorylated by the same or different protein kinases. Dependencies among phosphorylation sites in a single protein can be intricate , and the position and the number of phosphorylated residues can affect the biological outcome .
In the experimental dataset, we observed that the identified number of phosphorylation sites per protein was largely in agreement with the expected values, except for large values of phosphorylation sites and a single phosphorylation site. This observation was more noticeable for the predicted dataset. This suggests that there are at least three discrete regions in the distribution: i) a region of overrepresentation of single phosphorylation sites, ii) a region where the number of phosphorylation sites is proportional to the total number of S, T and Y; i.e., the more sites can be phosphorylated, the more will and proportionally, and, iii) a region where phosphorylation sites appear more often than expected given the total number of S, T, Y. Similar behavior was previously suggested to result from a rich-gets-richer process for the accumulation of phosphorylation sites , however the low coverage of the experimental dataset in this study does not allow us to arrive at such a conclusion. Nevertheless, the data in Figure 1, strongly suggests that once a single phosphorylation event happened in a protein, further phosphorylations will accumulate depending only on the abundance of phosphorylatable residues, until reaching a threshold whereafter further phosphorylations will happen even more rapidly than dictated by the abundance of phosphorylatable residues (longer tail).
Our finding that the phosphorylation hotspots occur preferentially outside conserved domains suggests that, indeed, they may serve as sites of signal integration as they are outside of regions such as catalytic domains or protein-protein interaction domains. Multisite phosphorylation occurring outside structured regions was shown for single nuclear proteins in several studies. This is the case for the human protein Ets-1. Multiple Ca2+-dependent phosphorylation sites in an unstructured flexible region of this transcription factor act additively to produce graded DNA binding affinity . Our result that nucleus-related GO terms are overrepresented in hotspot-containing proteins is in line with studies that indicate a central involvement of many nuclear proteins as integration hubs for phosphorylation-dependent signaling .
In an MS-based phosphoproteomics study in A. thaliana, it was suggested that the mRNA splicing machinery is a major target of protein phosphorylation . Our results support this hypothesis. One third (10/31) of the proteins with nine or more experimentally determined phosphorylation sites are involved in metabolic processes associated with nucleic acids, especially with "RNA splicing" (Table 1).
Disease resistance genes, S-locus proteins and receptors had previously been shown to display a high variability between different wild varieties of A. thaliana. In combination with our findings this indicates that receptors belong to the more variable proteins in A. thaliana accessions, and gains or losses of phosphorylation sites in rapidly evolving and variable regions of receptors could facilitate the evolution of kinase-signaling circuits . The importance of specific phosphorylation sites in A. thaliana receptor proteins for receptor dimer formation and activation of signaling events was concluded from experiments on the BRI1/BAK1 receptors . Similarly, in different human receptor proteins the importance of their site-specific phosphorylations could be demonstrated, for example in receptor tyrosine kinases of the ErbB family .
The potential contribution of the identified losses and gains of the phosphorylation sites by nsSNPs to adaptive responses of the various natural accessions will be an interesting field to be analyzed in future association studies.
By mapping nsSNPs onto phosphorylation sites, we identified losses and gains of phosphorylation sites, which can be important in adaptive responses of the natural accessions in their different environments. Especially receptor proteins were affected by losses of experimental phosphorylation sites. Based on the observed gains and losses of predicted phosphorylation sites it can be expected that beyond receptor proteins also other proteins involved in signaling and stress response are affected by changes, whereas proteins involved in metabolism, catalytic activity and biosynthesis are less affected. These findings suggest a relatively high variability of signal transduction-related proteins and receptors and more conserved regulation in metabolism. Since receptors and signaling processes are primarily involved in recognition and response to environmental cues, the overrepresentation of phosphorylation sites (gain or loss) in these functional classes indeed supports the view of nsSNPs as evolutionary means of adaptation.
Genome annotation and identification of protein domains
The A. thaliana Columbia-0 genome was sequenced in the year 2000 . In this study, we used the genome annotation provided by The Arabidopsis Information Resource, release 7.0 (TAIR7) . Protein domains were identified using the Pfam v23 library of Hidden Markov Models . 2,902 Pfam HMMs have significant hits in 19,904 protein-coding loci (23,760 protein models) in TAIR7.
Experimental phosphorylation sites
Experimental phosphorylation sites of different phosphoproteomic studies in A. thaliana[27–36] were taken from PhosPhAt (version 3.0) . The PhosPhAt database aims to collect manually curated phosphorylation motifs from A. thaliana. While PhosPhAt provides both, phosphorylation sites with uncertain and exactly defined positions within the respective protein sequence, only the latter were included in this study. The dataset comprised 7,178 unambiguously identified phosphopeptides identified in 4,252 protein-coding loci annotated in TAIR7 (with the following counts per residue type: 5,078 S; 1,482 T; 618 Y).
Predicted phosphorylation sites
The predictions of phosphorylation of S, T, and Y were extracted from PhosPhAt. PhosPhAt uses a Support Vector Machine (SVM) in order to reliably predict phosphorylation sites in A. thaliana. The SVM has been trained on experimentally verified phosphorylation sites from A. thaliana with the goal of specifically capturing the properties of plant phosphorylation sites and was shown to predict plant phosphorylation sites with a considerably better performance than other available predictors usually trained on non-plant species .
Overview of the number of SNPs per dataset.
Number of non-redundant SNPs in this study
Number of non-redundant SNPs mapping onto cDNAs
Number of non-redundant SNPs mapping onto CDS
Number of non-redundant SNPs causing at least one non-synonymous substitution
Number of non-redundant SNPs always causing synonymous substitutions
Mapping SNP positions onto the TAIR7 genome release and annotation
The datasets Nordborg2005 and Clark2007 were originally mapped onto previous versions of the A. thaliana genome sequence, thus the first step in the present study was to bring all three datasets to refer to the same reference coordinate system, i.e., TAIR7. We took the 30 basepairs (bp) of right and left flanking sequences of each SNP position, together with the SNP base, from the reported A. thaliana genome versions. Using MEGABLAST (W = 39, D = 3) [58, 59] we mapped them onto the TAIR7 genome sequence, allowing only 100% identical matches. None of the polymorphic positions were changed between the different releases of the A. thaliana genome. Subsequently, using the genome annotation provided by TAIR7, we determined which of these polymorphic positions occurred inside processed mRNAs and protein coding sequences. The latter is necessary to determine if the SNPs caused a synonymous or non-synonymous substitution, and allowed us to determine, which phosphorylation site positions were affected by SNPs.
Evaluating enrichment of gene ontology terms in sets of proteins
The gene ontology (GO) provides a set of controlled and structured vocabularies, i.e., ontologies, in three domains of molecular biology: cellular component, biological process and molecular function . A large proportion of A. thaliana genes in TAIR7 have been annotated with at least one GO term. In this study, we used a subset of these GO terms, known as the plant GO Slim, which provides a high level view of the above mentioned ontologies. Over- and underrepresentation analysis of plant GO Slim terms was carried out using the plugin BiNGO v2.3  for the software package Cytoscape . Statistically significant categories, either over- or underrepresented, were selected according to their corrected p-value (False Discovery Rate (FDR) ≤ 5E-2), using a hypergeometric test.
Effect of SNPs on phosphorylation sites
Differences between the effects of SNPs on phosphorylation sites and non-phosphorylation sites
We evaluated the differences between SNPs affecting phosphorylation- and non-phosphorylation sites by comparison of their distributions. For each natural accession from A. thaliana, we mapped all SNPs separately on the respective protein sequences.
Subsequently experimental sites were mapped onto the protein sequences, in which at least one SNP was found. In a third step, the distribution of SNPs mapping to phosphorylation sites and non-phosphorylation sites was evaluated for protein sequences with at least one SNP mapping onto an experimental phosphorylation site. For each of the potentially phosphorylated amino acid S, T and Y (phosphorylation sites and non-phosphorylation sites), we counted the number of synonymous and non-synonymous substitutions to all 20 amino acids and to stop-codons. We also counted the number of S, T, and Y phosphorylation sites and the total number of S, T, and Y in all proteins with at least one SNP mapping onto an experimental phosphorylation site. Based on this information, we created 2 × 2 contingency tables for each substitution pair and evaluated the significance (corrected p-value, FDR ≤ 5E-2) with a Fisher's exact test for each contingency table. Finally, we represented the enrichment and depletion of substitutions between phosphorylation sites and non-phosphorylation sites as the odds ratio between their probabilities.
The same procedure was applied to the sets of predicted phosphorylation sites.
Losses of experimental phosphorylation sites
The number of losses of experimental phosphorylation sites was computed by determining SNPs mapping to experimental phosphorylation sites and resulting in an exchange to a non-phosphorylatable amino acid.
Gains and losses of predicted phosphorylation sites
SNPs of predicted phosphorylation sites resulting in exchange of S, T or Y to a non-phosphorylatable amino acid were defined as "loss", and SNPs leading to an exchange of a non-phosphorylatable amino acid with a S, T, Y predicted to be phosphorylated with high confidence (decision value ≥1) were defined as "gain". Additionally, we also determined gains and losses of phosphorylation sites by SNPs considering the sequential context between -6 and +6 amino acids around the central residue (S, T, or Y). This sequential context can be affected by SNPs thus changing the probability of phosphorylation of the central S, T or Y. We used only positions with highly confident decision values ≥1 for phosphorylation sites and ≤ -1 for non-phosphorylation sites in the TAIR7 protein sequence considering the accession specific sequence. Thus, there are two types of gains/losses of predicted phosphorylation sites, (i) based on score, where the score of a phosphorylatable amino acid changes from ≥1 to ≤ -1 (loss) or vice versa (gain) due to an amino acid substitution within the phosphorylation site recognition motif, (ii) based on a change from one amino acid residue with high-confidence phosphorylation prediction score into a non-phosphorylatable amino acid (loss) or the creation of a peptide with a confidence prediction score greater than 1 (gain).
Three different datasets were used for an analysis of over- and underrepresentation of plant GO Slim terms among the proteins affected by gain or loss of phosphorylation sites: the first dataset contained only proteins that lost a predicted site; the second dataset consisted of proteins, which gained a predicted site; and the third dataset included proteins with both, gain and loss of predicted phosphorylation sites. These three datasets were compared against two reference sets, (i) a reference set that comprised all proteins containing a predicted phosphorylation site (score ≥ 1) and (ii) a reference set that comprised all A. thaliana proteins.
Identification of phosphorylation hotspots
Hotspots were computed based on two datasets: (i) experimentally verified phosphorylation sites, (ii) predicted phosphorylation sites. A hotspot was defined as a window of a given length, which (i) contains a significantly increased number of phosphorylation sites (for experimental sites) or (ii) has a significantly increased windows score (for predicted sites) compared to an empiric background distribution. Analyses were run using the following window sizes: (i) windows of 5, 10, 15, 20 amino acids length for experimental sites and (ii) windows of 10, 20, 30, 40 for predicted sites.
To generate background proteomes, proteins were sampled according to the length distribution and amino acid composition of all proteins in the A. thaliana proteome. Resulting proteins were randomly phosphorylated at S, T, and Y residues with probabilities as identified after mapping all experimental phosphorylation sites onto A. thaliana protein sequences in case of experimental sites. For predicted sites, a score was randomly assigned to the S, T, and Y residues by sampling a score from the corresponding distribution of prediction scores in the A. thaliana proteome. In this way, 10,000 background proteomes of size of the experimental set were generated for the experimental sites, 1,000 background proteomes of size of the A. thaliana proteome were generated for the predicted sites.
To generate empiric background distributions, each background proteome was analyzed by scanning each protein with a window of fixed size. Window scores were computed by using (i) the number of contained phosphorylated amino acid residues for experimental sites, or (ii) the sum of all scores in a window for predicted sites. For a given window size the empiric background distribution is formed by the distribution of window scores. Windows containing the same S, T, and Y residues of a protein were counted only once.
The proteome of A. thaliana with mapped experimental or predicted sites was scanned and window scores were determined accordingly. For predicted sites, only windows containing at least the expected number of S, T, and, Y sites based on the determined amino acid frequency in the A. thaliana proteome were taken into account for further analysis (in contrast to the background distribution).
The null-hypothesis of statistical testing of hotspots states: the score of a candidate window is derived from the described background distribution. The alternative hypothesis states: If the p-value of the score from a candidate window is less than the significance level (5E-2), then it is assumed that the window results from an unknown hotspot distribution. The right tail of background distribution is considered for testing. P-values were determined and Bonferroni corrected for multiple testing. Windows which had scores with corrected p-value less than 5E-2, were saved for further analysis.
Overlap of hotspots with protein domains
We tested the hypothesis whether hotspots are uniformly distributed across proteins, independently of protein domains. For the statistical test, (i) the number of hotspots overlapping with a protein domain was computed for the real data, (ii) the same number of hotspots of a given size was sampled onto the proteins, (iii) the hotspots overlapping with domains were counted and saved. This procedure was repeated for 10E7 times and the number of overlaps of the real data was compared to the empirically generated background distribution. The null-hypothesis was tested analogous as described in the previous section.
We thank the German Ministry for Education and Research (BMBF) for funding this work in the scope of GABI-FUTURE (grant 0315046) and the Max Planck Society. Liam Childs and Ronan Sulpice are acknowledged for helpful discussions and Flavia Vischi Winck for critical reading of the manuscript.
- Hunter T: The age of crosstalk: phosphorylation, ubiquitination, and beyond. Mol Cell. 2007, 28 (5): 730-738. 10.1016/j.molcel.2007.11.019.PubMedView ArticleGoogle Scholar
- Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006, 127 (3): 635-648. 10.1016/j.cell.2006.09.026.PubMedView ArticleGoogle Scholar
- Controlled vocabulary of posttranslational modifications. [http://www.expasy.org/cgi-bin/ptmlist.pl]
- Ptacek J, Snyder M: Charging it up: global analysis of protein phosphorylation. Trends Genet. 2006, 22 (10): 545-554. 10.1016/j.tig.2006.08.005.PubMedView ArticleGoogle Scholar
- de la Fuente van Bentem S, Hirt H: Using phosphoproteomics to reveal signalling dynamics in plants. Trends Plant Sci. 2007, 12 (9): 404-411. 10.1016/j.tplants.2007.08.007.PubMedView ArticleGoogle Scholar
- Colcombet J, Hirt H: Arabidopsis MAPKs: a complex signalling network involved in multiple biological processes. Biochem J. 2008, 413 (2): 217-226. 10.1042/BJ20080625.PubMedView ArticleGoogle Scholar
- Maathuis FJ: Conservation of protein phosphorylation sites within gene families and across species. Plant signaling & behavior. 2008, 3 (11): 1011-1013.View ArticleGoogle Scholar
- Sims RJ, Reinberg D: Is there a code embedded in proteins that is based on post-translational modifications?. Nat Rev Mol Cell Biol. 2008, 9 (10): 815-820. 10.1038/nrm2502.PubMedView ArticleGoogle Scholar
- Seet BT, Dikic I, Zhou MM, Pawson T: Reading protein modifications with interaction domains. Nat Rev Mol Cell Biol. 2006, 7 (7): 473-483. 10.1038/nrm1960.PubMedView ArticleGoogle Scholar
- Wang D, Harper JF, Gribskov M: Systematic trans-genomic comparison of protein kinases between Arabidopsis and Saccharomyces cerevisiae. Plant Physiol. 2003, 132 (4): 2152-2165. 10.1104/pp.103.021485.PubMed CentralPubMedView ArticleGoogle Scholar
- Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S: The protein kinase complement of the human genome. Science. 2002, 298 (5600): 1912-1934. 10.1126/science.1075762.PubMedView ArticleGoogle Scholar
- Kersten B, Agrawal GK, Durek P, Neigenfind J, Schulze W, Walther D, Rakwal R: Plant phosphoproteomics: an update. Proteomics. 2009, 9 (4): 964-988. 10.1002/pmic.200800548.PubMedView ArticleGoogle Scholar
- Peck SC: Phosphoproteomics in Arabidopsis: moving from empirical to predictive science. J Exp Bot. 2006, 57 (7): 1523-1527. 10.1093/jxb/erj126.PubMedView ArticleGoogle Scholar
- Kersten B, Agrawal GK, Iwahashi H, Rakwal R: Plant phosphoproteomics: a long road ahead. Proteomics. 2006, 6 (20): 5517-5528. 10.1002/pmic.200600232.PubMedView ArticleGoogle Scholar
- Durek P, Schmidt R, Heazlewood J, Jones A, MacLean D, Nagel A, Kersten B, Schulze W: PhosPhAt: The Arabidopsis thaliana phosphorylation site database. An update. Nucleic Acids Res. 2010, D828-834. 10.1093/nar/gkp810. 38 DatabaseGoogle Scholar
- Gao J, Agrawal GK, Thelen JJ, Xu D: P3DB: a plant protein phosphorylation database. Nucleic Acids Res. 2009, D960-962. 10.1093/nar/gkn733. 37 DatabaseGoogle Scholar
- Grimsrud PA, den Os D, Wenger CD, Swaney DL, Schwartz D, Sussman MR, Ane JM, Coon JJ: Large-scale phosphoprotein analysis in Medicago truncatula roots provides insight into in vivo kinase activity in legumes. Plant Physiol. 152 (1): 19-28. 10.1104/pp.109.149625.Google Scholar
- Yachie N, Saito R, Sugahara J, Tomita M, Ishihama Y: In silico analysis of phosphoproteome data suggests a rich-get-richer process of phosphosite accumulation over evolution. Mol Cell Proteomics. 2009, 8 (5): 1061-1071. 10.1074/mcp.M800466-MCP200.PubMed CentralPubMedView ArticleGoogle Scholar
- Malik R, Nigg EA, Korner R: Comparative conservation analysis of the human mitotic phosphoproteome. Bioinformatics. 2008, 24 (12): 1426-1432. 10.1093/bioinformatics/btn197.PubMedView ArticleGoogle Scholar
- Boekhorst J, van Breukelen B, Heck AJ, Snel B: Comparative phosphoproteomics reveals evolutionary and functional conservation of phosphorylation across eukaryotes. Genome Biol. 2008, 9 (10): R144-10.1186/gb-2008-9-10-r144.PubMed CentralPubMedView ArticleGoogle Scholar
- Thomson M, Gunawardena J: Unlimited multistability in multisite phosphorylation systems. Nature. 2009, 460 (7252): 274-277. 10.1038/nature08102.PubMed CentralPubMedView ArticleGoogle Scholar
- Benzeno S, Lu F, Guo M, Barbash O, Zhang F, Herman JG, Klein PS, Rustgi A, Diehl JA: Identification of mutations that disrupt phosphorylation-dependent nuclear export of cyclin D1. Oncogene. 2006, 25 (47): 6291-6303. 10.1038/sj.onc.1209644.PubMedView ArticleGoogle Scholar
- Borevitz JO, Hazen SP, Michael TP, Morris GP, Baxter IR, Hu TT, Chen H, Werner JD, Nordborg M, Salt DE, Kay SA, Chory J, Weigel D, Jones JD, Ecker JR: Genome-wide patterns of single-feature polymorphism in Arabidopsis thaliana. Proc Natl Acad Sci USA. 2007, 104 (29): 12057-12062. 10.1073/pnas.0705323104.PubMed CentralPubMedView ArticleGoogle Scholar
- Clark RM, Schweikert G, Toomajian C, Ossowski S, Zeller G, Shinn P, Warthmann N, Hu TT, Fu G, Hinds DA, Chen H, Frazer KA, Huson DH, Scholkopf B, Nordborg M, Ratsch G, Ecker JR, Weigel D: Common sequence polymorphisms shaping genetic diversity in Arabidopsis thaliana. Science. 2007, 317 (5836): 338-342. 10.1126/science.1138632.PubMedView ArticleGoogle Scholar
- Nordborg M, Hu TT, Ishino Y, Jhaveri J, Toomajian C, Zheng H, Bakker E, Calabrese P, Gladstone J, Goyal R, Jakobsson M, Kim S, Morozov Y, Padhukasahasram B, Plagnol V, Rosenberg NA, Shah C, Wall JD, Wang J, Zhao K, Kalbfleisch T, Schulz V, Kreitman M, Bergelson J: The pattern of polymorphism in Arabidopsis thaliana. PLoS biology. 2005, 3 (7): e196-10.1371/journal.pbio.0030196.PubMed CentralPubMedView ArticleGoogle Scholar
- Ossowski S, Schneeberger K, Clark RM, Lanz C, Warthmann N, Weigel D: Sequencing of natural strains of Arabidopsis thaliana with short reads. Genome Res. 2008, 18 (12): 2024-2033. 10.1101/gr.080200.108.PubMed CentralPubMedView ArticleGoogle Scholar
- Nuhse TS, Stensballe A, Jensen ON, Peck SC: Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database. Plant Cell. 2004, 16 (9): 2394-2405. 10.1105/tpc.104.023150.PubMed CentralPubMedView ArticleGoogle Scholar
- Wolschin F, Weckwerth W: Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites. Plant Methods. 2005, 1 (1): 9-10.1186/1746-4811-1-9.PubMed CentralPubMedView ArticleGoogle Scholar
- de la Fuente van Bentem S, Anrather D, Roitinger E, Djamei A, Hufnagl T, Barta A, Csaszar E, Dohnal I, Lecourieux D, Hirt H: Phosphoproteomics reveals extensive in vivo phosphorylation of Arabidopsis proteins involved in RNA metabolism. Nucleic Acids Res. 2006, 34 (11): 3267-3278. 10.1093/nar/gkl429.PubMedView ArticleGoogle Scholar
- Niittyla T, Fuglsang AT, Palmgren MG, Frommer WB, Schulze WX: Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis. Mol Cell Proteomics. 2007, 6 (10): 1711-1726. 10.1074/mcp.M700164-MCP200.PubMedView ArticleGoogle Scholar
- Benschop JJ, Mohammed S, O'Flaherty M, Heck AJ, Slijper M, Menke FL: Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis. Mol Cell Proteomics. 2007, 6 (7): 1198-1214. 10.1074/mcp.M600429-MCP200.PubMedView ArticleGoogle Scholar
- Carroll AJ, Heazlewood JL, Ito J, Millar AH: Analysis of the Arabidopsis cytosolic ribosome proteome provides detailed insights into its components and their post-translational modification. Mol Cell Proteomics. 2008, 7 (2): 347-369.PubMedView ArticleGoogle Scholar
- Nuhse TS, Bottrill AR, Jones AM, Peck SC: Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses. Plant J. 2007, 51 (5): 931-940. 10.1111/j.1365-313X.2007.03192.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Sugiyama N, Nakagami H, Mochida K, Daudi A, Tomita M, Shirasu K, Ishihama Y: Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis. Mol Syst Biol. 2008, 4: 193-10.1038/msb.2008.32.PubMed CentralPubMedView ArticleGoogle Scholar
- de la Fuente van Bentem S, Anrather D, Dohnal I, Roitinger E, Csaszar E, Joore J, Buijnink J, Carreri A, Forzani C, Lorkovic ZJ, Barta A, Lecourieux D, Verhounig A, Jonak C, Hirt H: Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis. J Proteome Res. 2008, 7 (6): 2458-2470. 10.1021/pr8000173.PubMedView ArticleGoogle Scholar
- Whiteman SA, Serazetdinova L, Jones AM, Sanders D, Rathjen J, Peck SC, Maathuis FJ: Identification of novel proteins and phosphorylation sites in a tonoplast enriched membrane fraction of Arabidopsis thaliana. Proteomics. 2008, 8 (17): 3536-3547. 10.1002/pmic.200701104.PubMedView ArticleGoogle Scholar
- Jones AM, MacLean D, Studholme DJ, Serna-Sanz A, Andreasson E, Rathjen JP, Peck SC: Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana. J Proteomics. 2009, 72 (3): 439-451. 10.1016/j.jprot.2009.02.004.PubMedView ArticleGoogle Scholar
- Li H, Wong WS, Zhu L, Guo HW, Ecker J, Li N: Phosphoproteomic analysis of ethylene-regulated protein phosphorylation in etiolated seedlings of Arabidopsis mutant ein2 using two-dimensional separations coupled with a hybrid quadrupole time-of-flight mass spectrometer. Proteomics. 2009, 9 (6): 1646-1661. 10.1002/pmic.200800420.PubMedView ArticleGoogle Scholar
- Reiland S, Messerli G, Baerenfaller K, Gerrits B, Endler A, Grossmann J, Gruissem W, Baginsky S: Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks. Plant Physiol. 2009, 150 (2): 889-903. 10.1104/pp.109.138677.PubMed CentralPubMedView ArticleGoogle Scholar
- Michard E, Dreyer I, Lacombe B, Sentenac H, Thibaud JB: Inward rectification of the AKT2 channel abolished by voltage-dependent phosphorylation. Plant J. 2005, 44 (5): 783-797. 10.1111/j.1365-313X.2005.02566.x.PubMedView ArticleGoogle Scholar
- Sugiyama N, Masuda T, Shinoda K, Nakamura A, Tomita M, Ishihama Y: Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications. Mol Cell Proteomics. 2007, 6 (6): 1103-1109. 10.1074/mcp.T600060-MCP200.PubMedView ArticleGoogle Scholar
- Molina H, Horn DM, Tang N, Mathivanan S, Pandey A: Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry. Proc Natl Acad Sci USA. 2007, 104 (7): 2199-2204. 10.1073/pnas.0611217104.PubMed CentralPubMedView ArticleGoogle Scholar
- Bodenmiller B, Mueller LN, Mueller M, Domon B, Aebersold R: Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Nat Methods. 2007, 4 (3): 231-237. 10.1038/nmeth1005.PubMedView ArticleGoogle Scholar
- Cohen P: The regulation of protein function by multisite phosphorylation--a 25 year update. Trends in biochemical sciences. 2000, 25 (12): 596-601. 10.1016/S0968-0004(00)01712-6.PubMedView ArticleGoogle Scholar
- Ferrarese A, Marin O, Bustos VH, Venerando A, Antonelli M, Allende JE, Pinna LA: Chemical dissection of the APC Repeat 3 multistep phosphorylation by the concerted action of protein kinases CK1 and GSK3. Biochemistry. 2007, 46 (42): 11902-11910. 10.1021/bi701674z.PubMedView ArticleGoogle Scholar
- Park KS, Mohapatra DP, Misonou H, Trimmer JS: Graded regulation of the Kv2.1 potassium channel by variable phosphorylation. Science. 2006, 313 (5789): 976-979. 10.1126/science.1124254.PubMedView ArticleGoogle Scholar
- Pufall MA, Lee GM, Nelson ML, Kang HS, Velyvis A, Kay LE, McIntosh LP, Graves BJ: Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region. Science. 2005, 309 (5731): 142-145. 10.1126/science.1111915.PubMedView ArticleGoogle Scholar
- Kodiha M, Tran D, Morogan A, Qian C, Stochaj U: Dissecting the signaling events that impact classical nuclear import and target nuclear transport factors. PLoS ONE. 2009, 4 (12): e8420-10.1371/journal.pone.0008420.PubMed CentralPubMedView ArticleGoogle Scholar
- Holt LJ, Tuch BB, Villen J, Johnson AD, Gygi SP, Morgan DO: Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution. Science. 2009, 325 (5948): 1682-1686. 10.1126/science.1172867.PubMed CentralPubMedView ArticleGoogle Scholar
- Yun HS, Bae YH, Lee YJ, Chang SC, Kim SK, Li J, Nam KH: Analysis of phosphorylation of the BRI1/BAK1 complex in arabidopsis reveals amino acid residues critical for receptor formation and activation of BR signaling. Molecules and cells. 2009, 27 (2): 183-190. 10.1007/s10059-009-0023-1.PubMedView ArticleGoogle Scholar
- Riese DJ, Gallo RM, Settleman J: Mutational activation of ErbB family receptor tyrosine kinases: insights into mechanisms of signal transduction and tumorigenesis. Bioessays. 2007, 29 (6): 558-565. 10.1002/bies.20582.PubMed CentralPubMedView ArticleGoogle Scholar
- The Arabidopsis Genome Initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature. 2000, 408 (6814): 796-815. 10.1038/35048692.View ArticleGoogle Scholar
- Swarbreck D, Wilks C, Lamesch P, Berardini TZ, Garcia-Hernandez M, Foerster H, Li D, Meyer T, Muller R, Ploetz L, Radenbaugh A, Singh S, Swing V, Tissier C, Zhang P, Huala E: The Arabidopsis Information Resource (TAIR): gene structure and function annotation. Nucleic Acids Res. 2008, D1009-1014. 36 DatabaseGoogle Scholar
- Finn RD, Mistry J, Tate J, Coggill P, Heger A, Pollington JE, Gavin OL, Gunasekaran P, Ceric G, Forslund K, Holm L, Sonnhammer EL, Eddy SR, Bateman A: The Pfam protein families database. Nucleic Acids Res. 2010, D211-222. 10.1093/nar/gkp985. 38 DatabaseGoogle Scholar
- Download site Clark et al. 2007 dataset. [ftp://ftp.arabidopsis.org/home/tair/Sequences/Perlegen_Array_Resequencing_Data_2007]
- Download site Ossowski et al. 2008 dataset. [http://1001genomes.org/downloads/]
- Riaño-Pachón DM, Nagel A, Neigenfind J, Wagner R, Basekow R, Weber E, Mueller-Roeber B, Diehl S, Kersten B: GabiPD: the GABI primary database--a plant integrative 'omics' database. Nucleic Acids Res. 2009, D954-959. 10.1093/nar/gkn611. 37 DatabaseGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215 (3): 403-410.PubMedView ArticleGoogle Scholar
- Zhang Z, Schwartz S, Wagner L, Miller W: A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000, 7 (1-2): 203-214. 10.1089/10665270050081478.PubMedView ArticleGoogle Scholar
- Ashburner M, Ball C, Blake J, Botstein D, Butler H, Cherry J, Davis A, Dolinski K, Dwight S, Eppig J, Harris M, Hill D, Issel-Tarver L, Kasarskis A, Lewis S, Matese J, Richardson J, Ringwald M, Rubin G, Sherlock G: Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000, 25 (1): 25-29. 10.1038/75556.PubMed CentralPubMedView ArticleGoogle Scholar
- Maere S, Heymans K, Kuiper M: BiNGO: a Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks. Bioinformatics. 2005, 21 (16): 3448-3449. 10.1093/bioinformatics/bti551.PubMedView ArticleGoogle Scholar
- Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T: Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 2003, 13 (11): 2498-2504. 10.1101/gr.1239303.PubMed CentralPubMedView ArticleGoogle Scholar
- Benjamini YaYH: Controlling the False Discovery Rate - a Practical and Powerful Approach to Multiple Testing. J R Stat Soc Series B Stat Methodol. 1995, 57 (1): 289-300.Google Scholar
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