Figure 4

Minimum spanning tree of 148 B. pertussis strains. A. Each circle (node) represents a different GC-type, indicated by the number in the middle of the circle. The size of the circle corresponds to the number of strains within the particular GC-type. The number along the edge reflects the phylogenic distance between each neighboring node. A thicker edge corresponds to a shorter phylogenic distance. Color is based on country of isolation: Green = Netherlands, Pink = Sweden, Yellow = Japan, Purple = Australia, Brown = Dutch vaccine strains 509 and 134, White = Tohama I strain, Wellcome 28 strain. B. Minimum spanning tree as in Figure 4A, color based on ptxP type. Green = ptxP1, Blue = ptxP2, Red = ptxP3, Turquoise = ptxP4, Yellow = ptxP6, Purple = ptxP8 and Darkblue = ptxP13, White = Tohama I strain. C. Minimum spanning tree based on MLST-types, MLST designation was based on the allele number in the order ptxP, fim3 and prn region 1 (e.g. MLST 113 represents strains with ptxP1, fim3-1 and prn3). Each circle represents a different MLST-type, indicated by the number in the middle of the circle. The number along the edge reflects the phylogenic distance between each neighboring node. A thicker edge corresponds to a shorter phylogenic distance. Color code is as described in Figure 4B.