Changes in expression of Wif1 , Sfrp1 , Casp14 , and Krt71 in Hr- transfected PAM212 cells. (A) Expression of Wif1, Sfrp1, Casp14, and Krt71 in normal PAM212 cells using RT-PCR. M: size marker. (B) Western blot analysis showing the HR protein expressed in Hr-transfected PAM212 cells. β-tubulin indicates equal amount of protein loading. (C) Regulation of Wif1, Sfrp1, Casp14, and Krt71 gene expression by HR in Hr-transfected PAM212 cells by real-time PCR. The Y-axis indicates the fold difference in relative expression levels of each gene in HR-overexpressing cells compared with controls. *p value < 0.05. Results are the average of three independent experiments conducted in duplicate.