Figure 4

Expression analysis of ClpB genes in various stages of rice plant based on the microarrays performed for rice. A. Expression of OsClpB genes in different parts of rice plants. B. Expression of OsClpB genes during developmental stages. In silico analysis shown in A and B was performed using the Genevestigator database. The analysis included data from a total of 166 array experiments, and included 28 different tissues, organs or growth stages. C. Expression analysis of rice class I Clp ATPase transcripts in response to cold, heat and oxidative stresses. Hierarchical cluster display of expression profiles for OsClpB genes showing differential expression in rice (color bar represents the log2 expression values; green color representing low level expression, yellow shows medium level expression and red signifies the high level expression). D. Q-PCR analysis of the transcript expression of ClpB-cyt, ClpB-c and ClpB-m genes. The bars represent transcript level fold change values with respect to unstressed 10-d-old seedlings. Standard errors of the biological replicates are shown as error bars. E. Semi-quantitative RT-PCR based transcript expression analysis of rice ClpB genes under different stress conditions. The following stress conditions were used; HS: heat shock treatment (45°C; 10', 20', 30' and 1 h). LT: low temperature stress (8 ± 2°C; 24 h and 48 h). SS: NaCl stress (150 mM; 3 h and 6 h). DS: PEG treatment (12% PEG8000; 3 h and 6 h). PCR was performed for 25 cycles and products were resolved on 1% agarose gel. F. Semi-quantitative RT-PCR based transcript expression analysis of rice ClpB genes upon application of 100 μM ABA. PCR was performed for 25 cycles and products were resolved on 1% agarose gel.